Lambda Protein Phosphatase (Lambda PP) P0753S from New England Biolabs

Product Specs
ItemLambda Protein Phosphatase (Lambda PP)
CompanyNew England Biolabs
Price $170.00 Inquire
Catalog NumberP0753S
Size20000 units
Molecule NameLambda protein phosphatase
ApplicationsProteomics & Glycomics
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAFV600E-Associated Mitochondrial Division in CancerAntibodies (Basel)April 20, 2026Shanon T. Nizard, Yiyang Chen, Madhavika N. Serasinghe, Ruben Fernandez-Rodriguez, Kamrin D. Shultz, Jesminara Khatun, Anthony Mendoza, Jesse D. Gelles, Juan F. Henao-Martinez, Ioana Abraham-Enachescu, et al.MA, USA, Cat. No. B1761). Lambda protein phosphatase (New England Biolabs, Ipswich, MA, USA, Cat. No. P0753S) was added (2 μL) for a total of 800 U/reaction. For untreated controls, 2 μL of molecular biology-grade

(2) Interaction of NDRG1 and MRE11 Modulates DNA Replication and RepairCancers (Basel)April 20, 2026Hanna M. Doh, Nina Kozlova, Zhipeng A. Wang, Hwan Bae, Philip A. Cole, Taru MuranenThymidine (T1895), and HT-DNA (D6898) were from Sigma. Restriction enzymes and Lambda Phosphatase (P0753S) were from New England Biolabs (NEB), Ipswich, MA, USA. Mirin (#HY-19959, Medchem Express, Monmouth

(3) Pyrazolylpyrimidinamines Decorated via Petasis Reaction as Small-Molecule Activators of the RNA-Degrading Ribonuclease IRE1αACS Bio & Med Chem AuApril 15, 2026Amrutha K. Avathan Veettil, Yang Liu, Leon Wagner, Oguz Hastürk, Nguyen Song Thu Huynh, Giorgia Mancino, Maria Beerbaum, Peng Wucolumn. The proteins were collected and incubated with Lambda protein phosphatase (New England BioLabs, P0753S) to remove the phosphate groups overnight at 4 °C. Then the dephosphorylated protein was further purified

(4) Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotus japonicusPlant CommunicationsApril 13, 2026Yanan Wang, Hanbin Bao, Yutao Lei, Zhongmin Zou, Lifa Yuan, Haoxing Li, Hui Zhu, Dawei Xin, Christian Staehelin, Yangrong Cao0.1% Nonidet P-40) and incubated in the absence or presence of 0.2 U/mL λ-PPase (New England Biolabs, P0753S) at 37°C for 10 min. Lysates were analyzed by immunoblotting using anti-Myc (BioLegend, 626808) and

(5) A tandem recruitment site in the pseudokinase scaffold PEAK3 is subject to phosphorylation-dependent regulation and cancer-associated mutationsJournal of Biological ChemistryMarch 10, 2026Tianyue Zhao, Jianmei Hou, Changyuan Hu, Thomas R. Cotton, Roger J. Dalyfollowing reagents/inhibitors were used in this study: lambda protein phosphatase (New England Biolabs, P0753S), animal-free recombinant human EGF (PeproTech, AF-100–15), insulin from bovine pancreas (Merck, I-1882),

(6) Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal openingNature CommunicationsMarch 6, 2026Shota Yamauchi, Saashia Fuji, Hiroki Ikuta, Naoyuki Sugiyama, Yutaka Kodama, Luca Distefano, Haruki Fujii, Kota Yamashita, Hinano Takase, Mika Nomoto, et al.phenylmethylsulfonyl fluoride (PMSF), 10 µM leupeptin, and 400 units of λ-PPase (New England Biolabs, P0753), and incubated at 30 °C for 60 min. After the reaction, SDS sample buffer was added to the λ-PPase-treated

(7) RNA localization to nuclear speckles follows splicing logicNucleic Acids ResMarch 6, 2026Li Wen, Mauricio A Arias, Xinqi Fan, Joyce Xie, Sneha Paul, Susan E Liao, Marek Sobczyk, Oded Regev, Jingyi Fei0.5 μl), and supernatant was collected and dephosphorylated using Lambda Protein Phosphatase (NEB, P0753S) according to manufacturer’s instructions. Samples were incubated at 30°C for 30 min and retained for

(8) Hierarchical membrane-chromatin tethering buffers nuclear envelope assembly against alterations in lipid fluxbioRxivMarch 2, 2026Sarah R. Barger, Sofia Sepúlveda, Han Yang, Marc Goudge, Shoken Lee, Neale D. Ridgway, Shirin Bahmanyarlambda protein phosphatase experiments were conducted according to the manufacturer’s instructions (NEB P0753S). Generation of whole worm lysate Prior to lysate generation, L4 worms were selected 24 hours prior.

(9) McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylationEMBO ReportsFebruary 5, 2026Marina Arbi, Margarita Skamnelou, Lydia Koufoudaki, Vasiliki Bakali, Spyridoula Bournaka, Sihem Zitouni, Stavroula Tsaridou, Ozge Karayel, Catherine G Vasilopoulou, Aikaterini C Tsika, et al.β-D-1-thiogalactopyranoside (IPTG),UBPBio,P1010 "Lambda protein phosphatase, Biolabs",New England Biolabs,Cat #P0753 Recombinant Xenopus PLK4 (PLX4),Dr. Monica Bettencourt-Dias Lab,N/A "SteadyRed Adenosine 5’-[γ-P32]

(10) Kinetochore clustering is mediated by Mps1 phosphorylation of conserved MELT motifs in Stu1bioRxivJanuary 29, 2026Darren R. Mallett, Mengqiu Jiang, Gianna M. Minnuto, Sue Bigginstreated with Buffer H 0.15 supplemented with 1 mM MnCl2 and 120 units of Lambda Protein Phosphatase (P0753, New England Biolabs, Ipswich, MA, USA). The second sample was treated the same, except phosphatase

(11) Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregationDevelopmental CellJanuary 14, 2026Catherine A. Tower, Gabriel Manske, Emily L. Ferrell, Dilara N. Anbarci, Kelsey Jorgensen, Binbin Ma, Mansour Aboelenain, Rajesh Ranjan, Saikat Chakraborty, Lindsay Moritz, et al.dried for 10–20 minutes at RT, then immediately incubated with lambda phosphatase (New England Biolabs, P0753S) for 2 hours at 30°C. Slides were then blocked for 30 minutes at RT in PBS + 1% FBS+ 3% BSA + 0.05%

(12) Identification and Regulation of a Hepatic Lipogenic MetabolonbioRxivJanuary 6, 2026Xiaotong Zhu, William B. McKean, Tam Tran, Xiaorong Fu, Jay D. Horton, Chai-Wan KimJinhai Yu, UT Southwestern Medical Center",N/A Lambda Protein Phosphatase,New England Biolabs,Cat# P0753L DL-Dithiothreitol,Sigma-Aldrich,Cat# 43819-5G Adenosine 5’-triphosphate magnesium salt,Sigma-Aldrich,Cat#

(13) Pervasive phenotypic effects of FBXO42 are promoted by regulation of PP4 phosphataseThe EMBO JournalJanuary 3, 2026Hongbin Yang, Paul Smith, Yingying Ma, Emily Southworth, Varun Gopala Krishna, Beatrice Salerno, Joseph Rowland, Alexander E P Loftus, Domenico Grieco, Iolanda Vendrell, et al.,Thermo Scientific,17904 HEPES,Sigma-Aldrich,H3375-500G Hygromycin B,Thermo Scientific,10687010 KCl,Sigma-Aldrich,P9541-1KG Lambda phosphatase,New England Biolabs,P0753S Lipofectamine 2000,Invitrogen,11668019 Methanol,Fisher Scientific,10164663 N-Ethylmaleimide (NEM),Sigma-Aldrich,E3876-5G NaF,Fluka

(14) NAT10 promotes cancer metastasis by modulating p300/CBP activity through chromatin-associated tRNAMol CellDecember 18, 2025Ruhul Amin, Ngoc-Han Ha, Tinghu Qiu, Ronald Holewinski, Khiem C. Lam, Amélie Daugherty-Lopès, Huaitian Liu, Andy D. Tran, Maxwell P. Lee, Thorkell Andresson, et al.Counting Beads,Thermo Fisher Scientific,Cat# C36950 Lambda protein phosphatase,New England Biolabs,Cat# P0753S 25% Gluteraldehyde solution,Millipore-Sigma,Cat# G5882 Proteinase K,Thermo Fisher Scientific,Cat#

(15) Morphological and Hyperphosphorylation Transitions of Nanoscale Tau Aggregates in Alzheimer's DiseaseAdvanced ScienceNovember 19, 2025Adriana N. Santiago‐Ruiz, Siewert Hugelier, Gabriela L. Correa, Charles R. Bond, Edward B. Lee, Melike LakadamyaliAssay To dephosphorylate tissue sections, New England Biolab's Lambda Phosphatase protocol (Catalog number P0753S) and previous literature were followed.[ 62 ] After sections were deparaffinized and the antigen retrieval

(16) A versatile cohesion manipulation system probes female reproductive age-related egg aneuploidyNature AgingNovember 3, 2025Jiyeon Leem, Tom Lemonnier, Ani Khutsaidze, Lei Tian, Xiaojun Xing, Suxia Bai, Timothy Nottoli, Binyam Mogessiecontaining 3% BSA (w/v) for 1 hour. Spreads were then incubated with λ-phosphatase (New England Biolabs, P0753S) at 30 °C for 2 hours. Immunostaining was performed by washing out the blocking solution with PBS, followed

(17) LRRK2 kinase-mediated accumulation of lysosome-associated phospho-Rabs in tauopathies and synucleinopathiesActa NeuropatholOctober 23, 2025Silas A. Buck, Tuyana Malankhanova, Samuel Strader, Eileen B. Ma, Sarah Yim, Harrison W. Pratt, John Ervin, Edward B. Lee, Shih-Hsiu J. Wang, Todd J. Cohen, et al.Brij 35, pH 7.5) with 1 mM MnCl2 provided by the Lambda Protein Phosphatase kit (New England Biolabs P0753S). Slices were then incubated in 1 × NEBuffer with 1 mM MnCl2, either with or without 10,000 units/mL

(18) The PP2A-B55α phosphatase is a master regulator of mitochondrial degradation and biogenesisSci AdvOctober 3, 2025Valentina Cianfanelli, Monica Nanni, Samantha Corrà, Sofia Mauri, David Sumpton, Sergio Lilla, Rossella De Cegli, Matteo Bordi, Giacomo Milletti, Caterina Ferraina, et al.described below, and diluted 1:3 in 1X NEBuffer for PMP, with or without λ-PP (New England Biolabs, P0753S). The reactions were incubated for 30 min at 30°C, and protein extracts were denatured and analyzed

(19) Abnormal O-glycan sialylation in the mPFC contributes to depressive-like behaviors in male miceSci AdvOctober 3, 2025Youngsuk Seo, Inwoong Song, Ki Jung Kim, Bomi Chang, Prajitha Pradeep, Woo Suk Roh, Woojin Won, Jinhyeong Joo, Myeongju Kim, Jae Cheol Jeong, et al.subsequent heat inactivation. To capture sialylated O-glycopeptides, PNGase F and Protein Phosphatase (NEB, P0753S) were used to remove N-glycans and phosphates. Samples were purified using C18-SPE, with peptides eluted

(20) Harnessing indole scaffolds to identify small-molecule IRE1α inhibitors modulating XBP1 mRNA splicingNature CommunicationsSeptember 26, 2025Yang Liu, Amrutha K. Avathan Veettil, Raphael Gasper, Mao Jiang, Leon Wagner, Oguz Hastürk, Peng Wuthe Q HP anion exchange column was incubated with Lambda protein phosphatase (New England BioLabs, P0753S) overnight at 4 °C, the dephosphorylation was confirmed by LC-MS. The unphosphorylated protein was further

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1/27/2020Lambda Protein Phosphatase Review SANTANU DEGood product with strong enzymatic action.

Lambda Protein Phosphatase (Lambda PP)

Lambda Protein Phosphatase (Lambda PP) from New England Biolabs

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New England Biolabs

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Lambda Protein Phosphatase (Lambda PP) from New England Biolabs

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