PCR Nucleotide Mix C1141 from Promega

Product Specs
ItemPCR Nucleotide Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC1141
FormatConcentration: 10mM each
Conjugate/TagUnconjugated
Molecule NameNucleotide Mix
Size200microl
Concentration10mM each

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) KLF5 controls subtype-independent highly interactive enhancers in pancreatic cancer to regulate cell survivalSci AdvMarch 20, 2026Thomas L. Ekstrom, Zhangshuai Dai, Julia Thiel, Akshay Kanakan, Bishakha Joyeeta Saha, Meghana Manjunath, Nadine Schacherer, Pavlos Bousounis, Emily L Siegler, Amro M. Abdelrahman, et al.manufacturer’s protocol with GoTaq DNA Polymerase (Promega, M3001) and PCR nucleotide mix (Promega, C1141). Lentiviral production and infection HEK293T cells were plated 24 hours before transfection to be

(2) Ear tissue as a diagnostic sample for pestivirus detection in semi-domesticated Eurasian tundra reindeer (Rangifer tarandus tarandus) in NorwayFrontiers in MicrobiologyNovember 20, 2025Ester Malmström, Morten Tryland, Thomas Passler, Alice Becker, Stine Bull-Aurbakken, Scott Silvis, Rachel Phillips, Shollie Falkenbergof 1X Green GoTaq Flexi buffer (Promega M891A), 4.5 mM MgCl2 (Promega A351H), 0.4 mM dNTPs (Promega C1141), 100 U M-MLV RT (Promega M170B), 1 U GoTaq DNA Polymerase (Promega M829B), 20 U RNasin Ribonuclease Inhibitor

(3) Increased Myocardial MARK4 Expression in Patients with Heart Failure and Sleep-Disordered BreathingInt J Mol SciApril 11, 2025Bettina Seydel, Philipp Hegner, Anna-Maria Lauerer, Sönke Schildt, Fatma Bayram, Maria Tafelmeier, Dominik Wermers, Leopold Rupprecht, Christof Schmid, Stefan Wagner, et al.(Promega Corporation, Madison, WI, USA catalog number C1181), PCR nucleotide mix (Promega, catalog number C1145), RNasin® ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog

(4) Blood and tissue HIV-1 reservoirs display plasticity and lack of compartmentalization in virally suppressed peopleNat CommunMarch 4, 2025Marion Pardons, Laurens Lambrechts, Ytse Noppe, Liesbet Termote, Sofie De Braekeleer, Jerel Vega, Ellen Van Gulck, Sarah Gerlo, Linos Vandekerckhove#M0323L), 0.5 μM of each primer (Second PCR F UMI, Second PCR R UMI), 1.5 μl of 10 mM dNTPs (Promega, #C1141), 10 μl of 5× LongAmp Taq Reaction Buffer in 50 μl. The following cycling conditions were used: 94 °C

(5) Microhomology-mediated end-joining Knock-In approaches to delete the allergenic domain of trout parvalbumin beta-1. Preliminary results in F0 animals and feedbackOpen Research EuropeFebruary 25, 2025Veronique Lebret, Cecile Duret, Amaury Herpin, Pierre-yves Rescanusing the jumpstart Taq DNA polymerase (Sigma (cat. D9307)), the PCR nucleotide mix from Promega (cat. C1145) and Parvb1 gene specific primers (Eurofins genomics) at 10μM each. For the exon skipping experiment,

(6) Myocardial DYRK1B Expression Is Increased in Patients with Impaired Cardiac Contractility and Sleep-Disordered BreathingAntioxidants (Basel)January 29, 2025Fatma Bayram, Philipp Hegner, Anna-Maria Lauerer, Sönke Schildt, Dominik Wermers, Maria Johanna Baier, Julian Mustroph, Maria Tafelmeier, Zdenek Provaznik, Christof Schmid, et al.5x reaction buffer (Promega, catalog number M531A), and PCR nucleotide mix (Promega, catalog number C1145) were incubated for 1 h at 37 °C. Gene expression was quantified using a TaqMan™ Fast Advanced Master

(7) Fishy business in Seattle: Salmon mislabeling fraud in sushi restaurants vs grocery storesPLOS ONENovember 6, 2024Jewel L. Garcia, Yennifer A. Gaspar, Angelique Djekoundade, Mhicca Dalere, Asmaa A. Al-awadi, Marjolene Allossogbe, Thania C. P. Allossogbe, Itzel S. Aparicio, Hannah N. Buller, Hera Beatrice F. Cadelina, et al.the Promega “GoTaq Flexi DNA Polymerase PCR” assay kit (#M8295) and Promega “PCR nucleotide mix” (#C1141). Fish DNA barcoding was achieved by ordering previously published primers from Integrated DNA Technologies,

(8) HIV-PULSE: a long-read sequencing assay for high-throughput near full-length HIV-1 proviral genome characterizationNucleic Acids ResearchNovember 10, 2023Laurens Lambrechts, Noah Bonine, Rita Verstraeten, Marion Pardons, Ytse Noppe, Sofie Rutsaert, Filip Van Nieuwerburgh, Wim Van Criekinge, Basiel Cole, Linos VandekerckhoveM0323L), 0.5 μM of each primer (First PCR F, First PCR R), 1.5 μl of 10 mM dNTPs (Promega, catalog no. C1141), 10 μl of 5× LongAmp Taq Reaction Buffer in 50 μl. The following cycling conditions were used: 94°C

(9) The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regenerationeLifeDecember 14, 2022Zhao XF, Huffman LD, Hafner H, Athaiya M, Finneran MC, Kalinski AL, Kohen R, Flynn C, Passino R, Johnson CN, et al.40 mM Tris-HCI (pH 5.5). For PCR, 1–5 µl of gDNA was mixed with 0.5 µl of 10 mM dNTP mix (Promega, C1141, Madison, WI), 10 µl of 25 mM MgCl2, 5 µl of 5X Green GoTaq Buffer (Promega, M791A), 0.2 µl of GoTaq DNA polymerase

(10) Centaur antibodies: Engineered chimeric equine-human recombinant antibodiesFrontiers in ImmunologyAugust 19, 2022Ronit Rosenfeld et al.were performed using Advantage 2 DNA polymerase mix and buffer (Takara, 639202); 10 mM dNTPs (Promega,C1145) and 0.5 µM of each primer [Desalted oligonucleotides (Sigma)] in a total of 25 µl volume. Total RNA

(11) A mosaic of conserved and novel modes of gene expression and morphogenesis in mesoderm and muscle formation of a larval bivalveOrganisms Diversity & EvolutionJuly 7, 2022Stephan M. Schulreich, David A. Salamanca-DÃaz, Elisabeth Zieger, Andrew D. Calcino, Andreas WanningerPromega, Madison, WI, USA), 1 × Go Taq Flexi Buffer (#M890A, Promega), PCR nucleotide mix (0.8 mM, #C1145, Promega), 1.25 mM MgCl2 (#A351H, Promega), and nuclease-free water (#R0581, Thermo Fisher Scientific,

(12) 1,25-Dihydroxyvitamin D3 suppresses CD4(+) T-cell effector functionality by inhibition of glycolysisImmunologyJuly 1, 2022Bishop EL, Gudgeon NH, Mackie GM, Chauss D, Roberts J, Tennant DA, Maslowski KM, Afzali B, Hewison M, Dimeloe S.Ribonuclease Inhibitor (Cat# N2611), oligo(dT) 15 Primer (Cat# C1101), and PCR Nucleotide Mix (Cat# C1141). SYBR Green PCR Master Mix (Applied Biosystems, Cat# 4309155) was used for qPCR analysis, the primers

(13) Extensive characterization of HIV-1 reservoirs reveals links to plasma viremia before and during analytical treatment interruptionCell ReportsApril 26, 2022Basiel Cole, Laurens Lambrechts, Zoe Boyer, Ytse Noppe, Marie-Angélique De Scheerder, John-Sebastian Eden, Bram Vrancken, Timothy E. Schlub, Sherry McLaughlin, Lisa M. Frenkel, et al.,Sigma Aldrich,Cat#P9416 "PBS, pH 7.2",Thermo Fisher Scientific,Cat#20012027 dNTP PCR nucleotide mix 10 mM 1mL,Promega,Cat#C1145 UltraPure DNase/RNAse-free water,Thermo Fisher Scientific,Cat#10977023 RNAse inhibitor,Takara,Cat#2313B ThermaSTOP

(14) A PGE₂-MEF2A axis enables context-dependent control of inflammatory gene expressionImmunityAugust 10, 2021Cilenti F, Barbiera G, Caronni N, Iodice D, Montaldo E, Barresi S, Lusito E, Cuzzola V, Vittoria FM, Mezzanzanica L, et al.,Thermo Fisher Scientific,10009D Random Primers,Thermo Fisher Scientific,48190011 PCR Nucleotide Mix,Promega,C1145 Digitonin,Promega,G9441; CAS: 11024-24-1 Proteinase K solution,Promega,MC5005; CAS: 39450-01-6 LIVE/DEAD

(15) A novel mouse model of obstructive sleep apnea by bulking agent-induced tongue enlargement results in left ventricular contractile dysfunctionPLOS ONEDecember 10, 2020Simon Lebek, Philipp Hegner, Christian Schach, Kathrin Reuthner, Maria Tafelmeier, Lars Siegfried Maier, Michael Arzt, Stefan WagnerRNA using random primers (Promega, catalog number C1181), PCR nucleotide mix (Promega, catalog number C1145), RNasin1 ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog

(16) Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridementeLifeDecember 2, 2020Kalinski AL, Yoon C, Huffman LD, Duncker PC, Kohen R, Passino R, Hafner H, Johnson C, Kawaguchi R, Carbajal KS, et al.,Thermo Fisher Scientific,65086614,1:500 Chemical Compound,Proteinase K,New England Biolabs,P8107S, Chemical Compound,10 mM dNTP mix,Promega,C1141, Chemical Compound,5X Green GoTaq Buffer,Promega,M791A, Chemical Compound,GoTaq DNA polymerase,Promega,M3005, Chemical

(17) HIV Rebound Is Predominantly Fueled by Genetically Identical Viral Expansions from Diverse ReservoirsCell Host & MicrobeSeptember 11, 2019Marie-Angélique De Scheerder, Bram Vrancken, Simon Dellicour, Timothy Schlub, Eunok Lee, Wei Shao, Sofie Rutsaert, Chris Verhofstede, Tessa Kerre, Thomas Malfait, et al.protocol,N/A Proteinase K 20mg/ml,Ambion /Lifetechnologies,Cat#AM2546 PCR nucleotide mix 10mM 1ml,Promega,Cat#C1145 6M guanidinium thiocyanate BioUltr,Sigma Aldrich,SKU 50980–50ml; CAS 593–84-0 Glycogen (from mussels)

(18) A Putative Role of Teneurin-2 and Its Related Proteins in AstrocytesFrontiers in NeuroscienceJune 27, 2019Gestter W. L. Tessarin et al.Reactions Control reactions were performed without RNA addition for RT-PCR or with RNA addition for PCR assays (#C1141, GoTaq Flexi DNA Polymerase, Promega, WI, USA), using at least 30–35 cycles in both assays. Data Analysis RT-PCR

(19) Prevalence and molecular characterization of Toxoplasma gondii DNA in retail fresh meats in CanadaFood and Waterborne ParasitologyDecember 1, 2018Asma Iqbal, Nicol Janecko, Frank Pollari, Brent DixonM890A, Wisconsin, USA), 200 μM of each of the four deoxynucleotide triphosphates (dNTP) (Promega, cat. # C1141, Wisconsin, USA), 50 pmol of each primer (Sigma, Oakville, ON), and 1.25 U of Go Taq Hot Start Polymerase

(20) Biochemical and molecular characterisation of exogenous cytokinin application on grain filling in riceBMC Plant BiologyDecember 1, 2018Binay Bhushan Panda, Sudhanshu Sekhar, Sushant Kumar Dash, Lamboder Behera, Birendra Prasad Shawengine (Bio-Rad) thermocycler was used for PCR. The reagents used were PCR nucleotide mix (Promega, C1141) and GoTaq® DNA Polymerase (Promega, M8291). The programme set for each PCR was 94 °C for 3 min, 30

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PCR Nucleotide Mix

PCR Nucleotide Mix from Promega

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PCR Nucleotide Mix from Promega

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