Fig 2: A large increase in LMP1 caused by IL4 suppressed CHOP-induced cell death(A) Farage cells were treated with 1 μg/mL CHOP alone or in combination with IL4 (50 ng/mL) or an anti-SLAMF1 antibody (500 ng/mL) for 2 days. The drug-treated cells were lysed, which was followed by SDS-PAGE and western blotting. The following antibodies were used: anti-SLAMF1, phospho-STAT3 (Tyr705), phospho-STAT6 (Tyr641), phospho-NF-κB p65 (Ser536), PARP, BCL-XL, MCL1, BCL6, Bcl-2, LMP1, TP53, and GAPDH. (B) Farage cells were treated with 0.1 or 1 μg/mL CHOP alone or in combination with IL4 (50 ng/mL). The drug-treated Farage cells were washed and incubated with an Fc blocker. To stain IRF4 and BCL6, the cells were fixed and permeabilized. Flow cytometry was then performed after incubating the cells with PE-conjugated anti-IRF4 or APC-conjugated anti-BCL6.