Description
Custom preparations, technical support, bulk quantities and aliquoting available.
Procedure: Ultracentrifugation performed to isolate Human Very Low Density lipoprotein (VLDL). Very Low Density Lipoprotein levels have been correlated with accelerated rates of atherosclerosis, and are elevated in a number of diseases and metabolic states. Human VLDL Inhibits DNA synthesis in lymphocytes activated by the nonspecific mitogen concanavalin A (Con A). Human VLDL are the next step down from chylomicrons in terms of size and lipid content. It transports endogenous triglycerides, phospholipids, cholesterol and cholesteryl esters. This functions as the body's internal transport mechanism for lipids.
VLDL is defined as the lipoprotein fraction with the density range of 0.93 to 1.006 g/mL. It is spherical particle and in normal individuals ranges 30 to 80 nm in diameter. The molecular weight is 10 to 8 107. Compare with LDL particles, VLDL particles are heterogeneous in size and composition. VLDL particles can be separated in Sf 20-60 and Sf60-400. VLDL consists of protein 10%, phospholipid 19%, free cholesterol 7%, cholesterol ester 10% and triglycerides 56%. Apolipoprotein contribution% apoB 37 apoC 50 apoE 13 Apo B-48 is the sole constituent apolipoprotein on VLDL. It is presumed that the cholesteryl esters and triglycerides form the hydrophobic core, which is surrounded by a surface coat of apolipoprotein B, free cholesterol and polar phospholipid components oriented toward the aqueous medium