Fig 1: Mutual exclusivity between FABP7 and UCP1 expression in primary breast cancer tissues. a Association of FABP7, UCP1, and hypoxia median score in the METABRIC (n = 1904, upper panels) and TCGA (n = 960, lower panels) breast cancer cohorts. X and y axes show UCP1 and FABP7 expression, respectively. Hypoxia median score quartiles are indicated using different colors (orange, green, blue, and purple). b Correlation analyses of hypoxia score with FABP7 (upper panels) and UCP1 expression (lower panels)
Fig 2: UCP1 caused focal depolarization of mitochondria. a Representative confocal microscopic images of UCP1-expressing cells (upper panels) and UCP1-negative cells (lower panels) acquired from FABP7 knockdowns (FABP7-Kd). UCP1 expression, polarized mitochondria (Mito Tracker), and nuclei are indicated in green, magenta, and blue, respectively. Scale bars; 10 μm. b Representative scatterplots of JC-1 assay. X and y axes show green (JC-1 monomer) and red (J-aggregate) fluorescence, respectively. The gate named depolarized was used for calculating the proportion of depolarized cells. c Proportion of depolarized cells in Ctrl and FABP7-Kd calculated through JC-1 assay. Error bars, SD; **p < 0.01, n = 6
Fig 3: Association of UCP1 expression with clinicopathological features of breast cancer patients. a Representative immunohistochemical images of UCP1 expression in normal mammary ducts and paired invasive cancer. Images in the gray boxes are displayed in the lower row with higher magnification. The arrow heads indicate ductal cells and cancer cells. Scale bars; 200 μm (upper row) and 50 μm (lower row). b Immunohistochemistry of UCP1 expression patterns in normal mammary grands and paired invasive cancer cells (n = 34). c Immunohistochemistry of UCP1 expression in invasive cancer cells with histological grade, tumor size, estrogen receptor (ER) status, and tumor subtypes (n = 34). Digits in the bars represent number of cases. The vertical axis represents proportion of cases with a given characteristic to sum of all cases (%)
Fig 4: FABP7 knockdown (FABP7-Kd) and hypoxic exposure induced UCP1 expression in cancer cells. a FABP7, PRDM16, PGC-1α, and UCP1 expression in controls (Ctrl) and FABP7-Kd after 48 h normoxia or hypoxia (0.1% O2). b Representative western blots of FABP7, UCP1, PRDM16, PGC-1α, phosphorylated CREB (pCREB), and CREB after 48 h normoxia or hypoxia (0.1% O2). UCP1 bands appeared at 32 kD (monomer) and 64 kD (dimer). Four different PRDM16 isoforms were detected. c UCP1 expression (green) in cells cultured under normoxia or hypoxia (0.1% O2) for 48 h. Nuclei were stained with DAPI (blue). Scale bars; 20 μm. d Proportion of UCP1-expressing cells in Ctrl and FABP7-Kd after 48 h normoxia or hypoxia (0.1% O2). Error bars, SD; *p < 0.05, **p < 0.01, ***p < 0.001; n = 3
Supplier Page from Abcam for Human UCP1 peptide