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Recombinant human EGF protein (Animal Free)
Recombinant human EGF protein (Animal Free) from Abcam
Be the first to write a review!
Citations:
(12)
Supplier Page
Supplier Page from
Abcam for
Recombinant human EGF protein (Animal Free)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Recombinant human EGF protein (Animal Free)
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab9697
Size
500 µg
Activity
Active
Applications
CellAct, FuncS, HPLC, SDS-PAGE, WB
NCBI Gene Aliases
HOMG4, URG
Gene Name
EGF
Source/Expression System
Escherichia coli
Species
Human
Research Area
Oncology
NCBI Full Gene Name
epidermal growth factor
Type
Proteins
Molecule Name
EGF
Format
Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg)
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Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (12)
(1)
Steric disruption of EGFR oligomerization overcomes therapy resistance in non-small cell lung cancer
Sci Adv
December 5, 2025
Daesun Song, Jaebong Jang, Michael Z. Lin, Yongwon Jung
hours post-
transfection
. For EGF-stimulated conditions, cells were treated with EGF (10 ng/ml) (
Abcam
,
ab9697
), and oligomerization dynamics were monitored for 0 to 120 min.
Live-cell imaging
was conducted in a
(2)
A conserved NR5A1-responsive enhancer regulates SRY in testis-determination
Nat Commun
March 30, 2024
Denis Houzelstein, Caroline Eozenou, Carlos F. Lagos, Maëva Elzaiat, Joelle Bignon-Topalovic, Inma Gonzalez, Vincent Laville, Laurène Schlick, Somboon Wankanit, Prochi Madon,
et al.
Transferrin, Selenium (ITS, 100X #12097549, Thermo Fisher Scientific), and EGF (20 ng/mL;
human
recombinant #
ab9697
,
Abcam
). Quantitative reverse transcription
polymerase chain reaction
(
qRT-PCR
) for undifferentiated
(3)
Targeting colorectal cancer with small-molecule inhibitors of ALDH1B1
Nature chemical biology
October 1, 2022
Feng Z, Hom ME, Bearrood TE, Rosenthal ZC, Fernández D, Ondrus AE, Gu Y, McCormick AK, Tomaske MG, Marshall CR,
et al
.
spheroid culture
medium (RPMI-1640 containing 20 ng/mL recombinant
human
epidermal growth factor (
Abcam
,
ab9697
), 20 ng/mL recombinant
human
fibroblast
growth factor (BioVision, 4037), 5 µg/mL heparin (Sigma), 1x
(4)
Posaconazole inhibits the stemness of cancer stem-like cells by inducing autophagy and suppressing the Wnt/β-catenin/survivin signaling pathway in glioblastoma
Frontiers in Pharmacology
August 11, 2022
Hua Wang, Yinfeng Tan, Hao Jia, Danqi Liu, Rangru Liu
KGA512), BrdU Cell Proliferation Assay Kit (Abcam, ab287841),
recombinant human EGF protein
(
Abcam
,
ab9697
), recombinant
human
FGF protein (
Abcam
,
ab9596
), B-27 (Gibco, 17504–044), N-2 (Gibco, 17502–048),
CD133
(5)
Engineering micro oxygen factories to slow tumour progression via hyperoxic microenvironments
Nature communications
August 2, 2022
Wang W, Zheng H, Jiang J, Li Z, Jiang D, Shi X, Wang H, Jiang J, Xie Q, Gao M,
et al
.
purchased from
Thermo Fisher Scientific
(Grand Island, NY, USA). TNF-α recombinant protein (
ab9642
), EGF (
ab9697
), anti-phospho-AKT (
ab38449
), anti-
mTOR
(
ab134903
), anti-phospho-mTOR (
ab109268
), anti-ERK (
ab32537
)
(6)
Alt-RPL36 downregulates the PI3K-AKT-mTOR signaling pathway by interacting with TMEM24
Nature communications
January 21, 2021
Cao X, Khitun A, Luo Y, Na Z, Phoodokmai T, Sappakhaw K, Olatunji E, Uttamapinant C, Slavoff SA.
(
Click Chemistry
tools, 1066-1000), Biotin-alkyne (
Click Chemistry
tools, 1266-5). Human EGF protein (
Abcam
,
ab9697
).
Cloning
and genetic constructs A construct comprising the full 5′UTR of
human
RPL36 transcript variant
(7)
Liposomal Delivery of Mitoxantrone and a Cholesteryl Indoximod Prodrug Provides Effective Chemo-immunotherapy in Multiple Solid Tumors
ACS nano
October 27, 2020
Mei KC, Liao YP, Jiang J, Chiang M, Khazaieli M, Liu X, Wang X, Liu Q, Chang CH, Zhang X,
et al
.
(#
ab18256
,
1: 200
), anti-perforin (#
ab16074
,
1:100
), anti-
granzyme B
(#
ab4059
,
1: 100
), and anti-IFN-γ (#
ab9697
,
1: 200
) were purchased from
Abcam
; (iii)
anti-IDO
(#
122402
,
1: 100
) was from
BioLegend
; (iv) anti-LC3B
(8)
Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis
Journal of Cancer
January 1, 2020
Deng QF, Fang QY, Ji XX, Zhou SW.
μmol/L gefitinib under standard culture conditions. PI3K assessments Cells were treated with EGF (
ab9697
;
Abcam
) or (
ab119139
;
Abcam
).
COX-2
was silenced using
shRNA
approaches (Gene Pharma Co). FLAG-
COX2
,
(9)
Hexokinase 2 Promotes Cell Growth and Tumor Formation Through the Raf/MEK/ERK Signaling Pathway in Cervical Cancer
Frontiers in oncology
January 1, 2020
Cui N, Li L, Feng Q, Ma HM, Lei D, Zheng PS.
p<0.01) were observed in HeLa-
HK2
and HeLa-
HK2
-FR cells. Additionally, the
recombinant human EGF protein
(
ab9697
,
Abcam
, USA) was used as an activator to active p-
ERK
expression in
HK2
knockdown
cells. As shown in
(10)
Epidermal Growth Factor and Adenosine Triphosphate Induce Natrium Iodide Symporter Expression in Breast Cancer Cell Lines
Open Access Macedonian Journal of Medical Sciences
August 6, 2019
Aisyah Elliyanti, Andani Eka Putra, Yunia Sribudiani, Noormartany Noormartany, Johan S. Masjhur, Tri Hanggono Achmad, Dachriyanus Dachriyanus
cells were incubated in serum-free medium overnight and followed by treatment of EGF 50 ng/ml (
Abcam
#
ab9697
), ATP 100μM (Sigma-Aldrich # 1388), and a combination of EGF and ATP, for 6, 12 and 24 hours. Each type
(11)
Long noncoding RNA LINC01089 predicts clinical prognosis and inhibits cell proliferation and invasion through the Wnt/β-catenin signaling pathway in breast cancer
OncoTargets and therapy
January 1, 2019
Yuan H, Qin Y, Zeng B, Feng Y, Li Y, Xiang T, Ren G.
only. Dovepress Yuan et al lines were cultured in
RPMI-1640 medium
(
Gibco
-BRL, Karlsruhe, Germany). EGF (
Abcam
,
ab9697
) was added at a final concentration of 10 ng/ml.17 MCF-10A cells were cultured as previously described.20
(12)
Application of an E. coli signal sequence as a versatile inclusion body tag
Microbial cell factories
March 21, 2017
Jong WS, Vikström D, Houben D, van den Berg van Saparoea HB, de Gier JW, Luirink J.
chemicals were purchased from
Sigma-Aldrich
. The rabbit polyclonal antisera against recombinant
hEGF
(
ab9697
) and TrxA (
T0803
) were from
Abcam
and Sigma Aldrich, respectively. Rabbit polyclonal antisera against
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Recombinant human EGF protein (Animal Free) from Abcam
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