Fig 1: MMP-2 activity is reduced during the sustained depressive-like state. (a) In-gel zymography assays (Zy) were performed at 72 h and 8 weeks after social defeat. (b) No difference in MMP-2 or MMP-9 activity was present shortly after social defeat; n = 6 animals per group. (c) At week 8 post-defeat, decreased MMP-2 activity was found in the SDPS group, without MMP-9 regulation; n = 6 animals (control); n = 4 (SDPS). *P < 0.05. Data are expressed as mean ± SEM. (d) Examples with equal loading showing the mature form of MMP-2 and MMP-9 (white bands), and the position of the 75 kDa marker band for each time point. Full gels are available in Supplementary Fig. 6 online.