Fig 1: Effect of recombinant human MMP-3 on paracellular permeability in HTM and SCEC cell monolayers. SCEC and HTM cells were treated with 10 ng/ml recombinant MMP-3 for 24 h, using PBS and inactivated MMP-3 (incubation with TIMP-1, MMP(-)) as vehicle and negative controls respectively. (A) SCEC and (B) HTM both show reductions in TEER values after treatment of 4.6 [2.9, 6.2] and 5 [2.2, 7.8] Ohms.cm2 respectively. Permeability to a 70 kDa dextran was increased in treated cells (MMP (+)) in both (C) SCEC and (D) HTM. (E) An average viability of 85% was expected for SCEC with MMP-3 concentrations up to 36 ng/ml. (F) 85% viability is retained on average in HTM cells at concentrations up to 151 ng/ml MMP-3. A, C and E in blue represent SCEC data, whereas B, D and F in red represent HTM data.
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