Fig 1: Genes with imputed locations on the surface of the genome-reduced bacteria and increased binding of a monoclonal antibody against a recombinant antigen expressed on the surfaces of genome-reduced bacteria transformed with pRAIDA2. (A) Genes removed with imputed locations on the surface of E. coli strains ME5000, ME5110, ME5119, and ME5125 as a function of percent genome deleted. (B) Binding of a commercial anti-HA monoclonal antibody to the surfaces of the genome-reduced bacteria as a function of percent genome deleted. (C) Immunoblots of extracts from the wild-type and genome-reduced bacteria, transformed with pRAIDA2, and with expression of the HA immunotag with or without rhamnose induction (indicated at the top). Also indicated is whether the bacteria were pretreated with trypsin prior to protein extraction. Rhamnose induces expression of the HA immunotag expressed via the AT expression cassette. Trypsin treatment removes the HA immunotag from the bacterial surface. DNAK was probed as a loading control. The normalized amount of AIDA-HA differed by <16% from strain to strain, with the ME5125 29.7% deleted strain expressing the least normalized AIDA-HA.