BglII from New England Biolabs

Product Specs
ItemBglII
CompanyNew England Biolabs
Price $267.00 Inquire
Catalog NumberR0144L
Concentration50 u/µL
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

A/GATCT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) 1-Deoxysphingolipids Require Very-Long-Chain Ceramide Synthesis to Induce ER Stress and NeurotoxicitybioRxivDecember 8, 2025Colleen Byrnes, Benjamin A. Clarke, Hongling Zhu, Saurav Majumder, Mari Kono, Richard L. ProiaTECR and CERS2 to allow for screening of indels. The restriction enzyme BglII (New England Biolabs, R0144L) was used to determine the absence or presence of indels in CERS2. The following primers were used for

(2) Multiscale characterization of Caenorhabditis elegans mutants to probe functional mechanisms of human actin pathological variantsiScienceNovember 21, 2025Théo Hecquet, Nadine Arbogast, Delphine Suhner, Anaïs Goetz, Grégory Amann, Selin Yürekli, Fiona Marangoni, Sophie Quintin, Johannes N. Greve, Nataliya Di Donato, et al.,New England Biolabs,R0161L Restriction enzyme: HaeIII,New England Biolabs,R018L Restriction enzyme: BglII,New England Biolabs,R0144L Restriction enzyme: AlwI,New England Biolabs,R0513S Restriction enzyme: MspA1I,New England Biolabs,R0577S Cas9,homemade,– F-127,Sigma-Aldrich,P2443;

(3) Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence proteinJournal of Advanced ResearchJune 24, 2025Dong Gil Lee, Hae-Jun Yang, Unbin Chae, Hong Jun Lee, Dong-Seok Lee, Kyung-Sook Chung, Taeho Kwon, Hakjin Kim, In-Sung Song, Young-Ho Park, et al.mutant EGFP genes (#1 ∼#4) and EGFP were cloned into the pCAGGS vector linearized with BglⅡ (Cat. #R0144L, NEB, Ipswich, MA, USA) using the In-Fusion HD Cloning Kit (#102518, Takara Bio, Shiga, Japan). To integrate

(4) Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in riceThe EMBO JournalMay 19, 2025Lijie Liu, Jianqin Hao, Ke Huang, Penggen Duan, Baolan Zhang, Zhihai Chi, Xiaohong Yao, Yunhai Li,New England Biolabs,M0202S Aar I,Thermo Fisher Scientific,ER1581 Kpn I,New England Biolabs,R3142L Bgl II,New England Biolabs,R0144L EcoR I,New England Biolabs,R3101S Sac I,New England Biolabs,R3156L Pst I,New England Biolabs,R0140S Nco

(5) Clinically translatable mitochondrial gene therapy in muscle using tandem mtZFN architectureEMBO Molecular MedicineApril 9, 2025Pavel A Nash, Keira M Turner, Christopher A Powell, Lindsey Van Haute, Pedro Silva-Pinheiro, Felix Bubeck, Ellen Wiedtke, Eloïse Marques, Dylan G Ryan, Dirk Grimm, et al.,New England Biolabs,R3101L BamHI,New England Biolabs,R3136L EagI,New England Biolabs,R3505L BglII,New England Biolabs,R0144L T4 DNA Ligase,New England Biolabs,M0202T High glucose Dulbecco’s modified Eagle media (DMEM),Thermo Fisher Scientific

(6) Case Report: Functional investigation of the γENaC G532S mutation presenting as mild PHA-1B3Frontiers in medicineJanuary 1, 2025Centonze E, Girish M, van Bemmelen MX, Staub O, Subramaniam G, Kellenberger S.with human ENaC. α-ENaC was transcribed from the pSDEasy vector, which was linearized with BglII (Cat #R0144L, New England Biolabs). β-ENaC was transcribed from the pBSK(+) Xglob construct, and γENaC from the pSD5

(7) Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Increases Cell Proliferation and Drug ResistanceMethods in Molecular BiologyJuly 11, 2024Xiongjie Jin, Demetrius Moskophidis, Nahid F. Mivechi(New England Biolabs, cat no. R3156L) BglII Restriction Endonuclease (New England Biolabs, cat no. R0144L) NotI Restriction Endonuclease (New England Biolabs, cat no. R0189L) Plasmids: BAC clone PR23-266H9

(8) Novel Enhanced Mammalian Cell Transient Expression Vector via Promoter CombinationInternational Journal of Molecular SciencesFebruary 16, 2024SunKyung Yoon, SeJin Park, JuneWoo Lee, Byoungguk Kim, WonSeok Gwaktreated with the restriction enzymes EcoR V (R3195L, New England Biolabs, Ipswich, MA, USA)/Bgl II (R0144L, New England Biolabs, Ipswich, MA, USA), and WPRE was subcloned to produce Group #3 recombinant plasmids

(9) Changes in searching behaviour of CSL transcription complexes in Notch active conditionsLife Sci AllianceDecember 14, 2023Sarah Baloul, Charalambos Roussos, Maria Gomez-Lamarca, Leila Muresan, Sarah Braydigested using AvrII (R0174S; New England Biolabs) and MluI (R0198S; NEB) for Su(H)::Halo, or BglII (R0144L; NEB) and XbaI (R0145S; NEB) for Hairless::Halo. The resulting AttB plasmids were injected into a strain

(10) Lack of evidence for ribosomal frameshifting in ATP7B mRNA decodingMolecular CellOctober 6, 2022Gary Loughran, Alla D. Fedorova, Yousuf A. Khan, John F. Atkins, Pavel V. Baranov2000,Invitrogen,11668-027 Optimem,Invitrogen,51985-026 L-glutamine,Sigma,G7513 Pen/Strep,Sigma,P4333 FBS,Sigma,F7524 DMEM,Sigma,D6429 Restriction enzyme XhoI,NEB,R0146L Restriction enzyme BglII,NEB,R0144L Restriction enzyme BamHI-HF,NEB,R3136L Phusion® High-Fidelity DNA Polymerase,NEB,M0530L T4 DNA ligase,NEB,M0202S Passive

(11) LncRNA HOTAIR sponges miR-301a-3p to promote glioblastoma proliferation and invasion through upregulating FOSL1Cellular signallingJune 1, 2022Guo S, King P, Liang E, Guo AA, Liu M.MluI [New England Biolabs Inc. (NEB), Ipswitch, MA, NEB, cat. no. R0198L] and BglII (NEB, cat. no. R0144L) sites. Primers for amplification are listed in Table 1. For mutated construct, mutant FOSL1 or mut-FOSL1,

(12) rigrag: high-resolution mapping of genic targeting preferences during HIV-1 integration in vitro and in vivoNucleic Acids ResearchJuly 21, 2021Gregory J Bedwell, Sooin Jang, Wen Li, Parmit K Singh, Alan N Engelmanendonucleases MseI (New England Biolabs, Ipswich, MA; catalogue number R0525L) and BglII (catalogue number R0144L) were used to fragment genomic DNA for integration site sequencing. Sequences of DNA oligonucleotides

(13) Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniaeGenes (Basel)May 22, 2019Lance E. Keller, Anne-StÃ©phanie Rueff, Jun Kurushima, Jan-Willem Veening

(14) Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA IntegrationCell Host & MicrobeSeptember 1, 2018Vasudevan Achuthan, Jill M. Perreira, Gregory A. Sowd, Maritza Puray-Chavez, William M. McDougall, Adriana Paulucci-Holthauzen, Xiaolin Wu, Hind J. Fadel, Eric M. Poeschla, Asha S. Multani, et al.#R0199L SpeI,New England Biolabs,Cat #R0133L MseI,New England Biolabs,Cat #R0525L BglII,New England Biolabs,Cat #R0144L Gibson assembly master mix,New England Biolabs,Cat #E2611L Critical Commercial Assays,Critical Commercial

(15) Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer CellsInternational journal of molecular sciencesFebruary 16, 2018Miano V, Ferrero G, Rosti V, Manitta E, Elhasnaoui J, Basile G, De Bortoli M.DNA. DNA digestion will be done by adding a total of 450 U of restriction enzyme (BglII and NsiI, NEB R0144L and R0127L respectively) overnight at 37 ◦ C while shaking at 300 rpm. A 4.5 µL aliquot was taken by

(16) Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clustersScience advancesJune 1, 2017Yuen KC, Slaughter BD, Gerton JL.and incubated at 37°C for 3 hours while shaking at 900 rpm. After quenching the SDS, 200 U of Bgl II (R0144L, NEB) was added and incubated at 37°C for 4 hours while shaking at 900 rpm. Additional 200 U of Bgl

(17) Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 GenerationPLoS ONESeptember 25, 2014Rolf B. Edvardsen, Sven Leininger, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius

(18) Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteinsCytotechnologyMarch 1, 2013Kotomi Nagahashi, Kazuo Umemura, Naohiro Kanayama, Takayuki Iwaki

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10/2/2015High Quality Restriction Enzyme for Cloning Prabhakaran KumarI used this BglII restriction enzyme along with EcoRV for the cloning of my gene of interest into a plasmid vector. Subsequently, I also used this enzyme for colony screening. This enzyme completely ... Read Review

BglII

BglII from New England Biolabs

Sequence

New England Biolabs

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BglII from New England Biolabs

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