BglII from New England Biolabs

Product Specs
ItemBglII
CompanyNew England Biolabs
Price $65.00 Inquire
Catalog NumberR0144S
Quantity2000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

A/GATCT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapat#R0189S) were employed; Δ UP (upstream region) was achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S); Δ TATA promoter using SbfI-HF (NEB, #R3642S) and BglII. For insertion of RMND5A and ANAPC1 sequences

(2) Human respiratory syncytial virus regulates the expression of interferon-stimulated genes through modulation of fibrillarinFront Cell Infect MicrobiolApril 15, 2026José Manuel Ulloa-Aguilar, Victor Javier Cruz-Holguin, Yazmín Rocío Benítez-Zeferino, Edgar Rodrigo Guzmán-Bautista, Julio García-Cordero, Luis Adrián De Jesús-González, Julio Angel Vázquez-Martínez, Edgar Ricardo Vázquez-Martínez, Luis Herrera-Moro Huitron, Alfredo Mosqueda-Gracida, et al.and the PCR-amplified insert were digested with the restriction enzymes BglII (New England Biolabs, #R0144S) and HindIII (New England Biolabs, #R0104S). Following digestion, the plasmid vector was dephosphorylated

(3) Generation of Nano-Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular VesiclesJ Extracell BiolApril 7, 2026Alexis Dogliani, Laura Fradale, Céline Valeille, Christian Cambillau, Emmanuelle Jacquin-Joly, Alain Roussel, Josiane Kassis-Sahyounvector, which carries ampicillin resistance, was digested with the restriction enzymes BglII (NEB #R0144S) and EcoRI (NEB #R0101S) for 1 h at 37°C. The linearized vector was then separated from the mCherry

(4) Chromatin dynamics of the Klf4 locus in mouse pluripotent cellsScientific ReportsMarch 27, 2026Jente van Staalduinen, Hélène Kabbech, Selçuk Yavuz, Ridvan Cetin, Agnese Loda, Wiggert van Cappellen, Adriaan Houtsmuller, Kerstin Wendt, Ihor Smal, Frank Grosveldvectors of all genomic regions were sequentially digested with BglII (New England Biolabs, Catalog no: R0144S) and I-SceI (New England Biolabs, Catalog no: R0694L). The final ANCH3 homology donor constructs were

(5) A bicistronic viral genome uses a compact type IV IRES near its 3′ end to express a transmembrane proteinCell ReportsFebruary 24, 2026Madeline E. Sherlock, Katherine E. Segar, Jeffrey S. Kieftproteins","Chemicals, peptides, and recombinant proteins" BglII restriction enzyme,New England Biolabs,R0144S PspXI restriction enzyme,New England Biolabs,R0656S T4 DNA ligase enzyme,New England Biolabs,M0202S Q5 site-directed mutagenesis kit

(6) Complexoform-restricted covalent TRMT112 ligands that allosterically agonize METTL5Nature Chemical BiologyJanuary 8, 2026F. Wieland Goetzke, Steffen M. Bernard, Cheng-Wei Ju, Jonathan Pollock, Kristen E. DeMeester, Jacob Gross, Gabriel M. Simon, Chuan He, Bruno Melillo, Benjamin F. CravattIDT. The ORF of TRMT112 was cloned into a pACYCDuet-1 vector using NdeI (NEB, R0111S) and BglII (NEB, R0144S) restriction and Gibson assembly (NEB, E2621S). Mutagenesis was carried out using a Q5 site-directed mutagenesis kit

(7) A droplet microfluidics-based platform for generating target-specific, natively-paired immune libraries and identifying potent and developable antibodiesScientific ReportsJanuary 3, 2026Anusuya Ramasubramanian, Brendan T. Deveney, Sarah A. Clark, Paula Boutin, Kayla Keepseagle, Saleh Riahi, Taotao Lao, Megan Salemi, Sagar Kathuria, Pep Amengual-Rigo, et al.double digested with the restriction enzymes BstAPI (NEB catalog no. R0654S) and BglII (NEB catalog no. R0144S) and subsequently PCR-purified (Macherey–Nagel, catalog no. 740609). Following double digestion and

(8) Epromoters bind key stress-related transcription factors to regulate clusters of stress response genesThe EMBO JournalJanuary 3, 2026Juliette Malfait, Jing Wan, Himanshu Narayan Singh, Charbel Souaid, Gaëlle Farah, Junhua Su, Magali Torres, Iris Manosalva, Nathalie Sakakini, Cyril Esnault, et al.kit,Promega,E1980 BamHI-HF,New England Biolabs,R3136S SalI-HF,New England Biolabs,R3138S BglII,New England Biolabs,R0144S HindIII,New England Biolabs,R0104S Alt-R CRISPR-Cas9 System,Integrated DNA Technologies,075915 Alt-R HDR Enhancer V2

(9) Protocol for screening anti-amyloidogenic compounds in cultured mammalian cellsSTAR ProtocolsDecember 1, 2025Emma Lacroix, Sandra Keerthisinghe, Evgenia A. Momchilova, Timothy E. Audas(PEI) linear, M.W. 25,000",Thermo Fisher Scientific,Cat # 043896.01 BglII,New England Biolabs,Cat # R0144S rCutSmart buffer,New England Biolabs,Cat # B6004S Dulbecco’s modified Eagle’s medium (DMEM),Corning,Cat

(10) Defining a Simplified Process in Yeast for Production of Enveloped VLP Dengue VaccineBioengineering (Basel)September 5, 2025Salomé de Sá Magalhães, Stephen A. Morris, Shinta Kusumawardani, Acep Riza Wijayadikusumah, Neni Nurainy, Eli Keshavarz-MoorePlasmid Maxi Kit (Qiagen, Germantown, MD, USA). The pAO815_Den1Den2 plasmid was linearized with BglII (R0144S, NEB, Jakarta, Indonesia) and transformed into Komagataella phaffii GS115 (his4, MutS) by electroporation.

(11) Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cellsMolecular OncologySeptember 1, 2025Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, et al.The pEGFP‐C2 TGFβR1 vector was then digested with EcoRI (R3101S; NEB, Ipswich, MA, USA) and BglII (R0144S; NEB, Ipswich, MA, USA), and the insert was ligated into the pHIV‐Zsgreen vector (Addgene plasmid #18121;

(12) Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technologyNat CommunJuly 18, 2025De Xing, Tao Bai, Ozlem Neyisci, Seyedeh Zahra Paylakhi, Alexander J. Duval, Yasemin Tekin, Mazhar AdliAAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR (Addgene, 60228) using restriction enzymes BglII (New England Biolabs, R0144S,) and MfeI-HF. pSH-EFIRES-P-AtAFB2-mCherry-weak NLS plasmid was obtained from Addgene (129717). The

(13) Rice Peroxygenase-9 Negatively Regulates Production of Reactive Oxygen Species and Increases Cellular Resistance to Abiotic StressInt J Mol SciJuly 18, 2025Anh Duc Tran, Kyoungwon Cho, Manh An Vu, Jeong-Il Kim, Hanh Thi Thuy Nguyen, Oksoo HanpCAMBIA-Cas9 binary vector [65] were double-digested with HindIII (Cat. #R3104, NEB) and BglII (Cat. #R0144S, NEB) overnight at 37 °C, separated by gel electrophoresis, extracted, and then ligated with the ratio

(14) Single cell transcriptomes of zebrafish germline reveal progenitor types and feminization by Foxl2leLifeJune 11, 2025Chen-wei Hsu, Hao Hao, Ching-Hsin Yang, Yan-wei Wang, Ker-Chau Li, Bon-Chu ChungNo. M180A), 5 U BsmBI (New England Biolabs, Cat. No. R0580), 3 U BglII (New England Biolabs, Cat. No. R0144S), and 6 U SalI (New England Biolabs, Cat. No. R0138S) were incubated with the following condition: 20

(15) Comprehensive benchmarking of genome editing quantification methods for plant applicationsiScienceApril 29, 2025Zheng Gong, Yan Zhang, Di Xia, Sohye Yoon, Peter Alexander Crisp, José Ramón Botella,New England Biolabs,R3193S BcoDI,New England Biolabs,R0542S BsrDI,New England Biolabs,R0574S BglII,New England Biolabs,R0144S MfeI-HF,New England Biolabs,R3589S RsaI,New England Biolabs,R0167S BsrDI,New England Biolabs,R0574S HpaI,New England Biolabs

(16) Enhancing the stability of continuous fermentations for platform chemical productioniScienceMarch 21, 2025Victoria Outram, Andrew Yiakoumetti, Charlotte Green, Rebekah King, John M. Ward, Alex ConradieLtd,R3136S SbfI (SbfI-HF variant),New England Biolabs UK Ltd,R3642S BglII,New England Biolabs UK Ltd,R0144S SapI,New England Biolabs UK Ltd,R0569S T4 DNA Ligase,New England Biolabs UK Ltd,M0202S GenElute™

(17) A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactionsCell ReportsFebruary 25, 2025Madeline E. Sherlock, Conner J. Langeberg, Katherine E. Segar, Jeffrey S. Kieftproteins","Chemicals, peptides, and recombinant proteins" BglII restriction enzyme,New England Biolabs,R0144S PspXI restriction enzyme,New England Biolabs,R0656S T4 DNA ligase enzyme,New England Biolabs,M0202S Q5 site-directed mutagenesis kit

(18) THUMPD3 regulates alternative splicing of ECM transcripts in human lung cancer cells and promotes proliferation and migrationPLOS ONEDecember 10, 2024Marie Klimontova, Han Zhang, Francisco Campos-Laborie, Natalie Webster, Byron Andrews, Kimberley Chung Kim Chung, Ryan Hili, Tony Kouzarides, Andrew J. Bannisterthe pCMV6-Entry (pEV) and pCMV6-THUMPD3 (pD3.3) (S3 Table) were linearised using BglII enzyme (NEB, R0144S) according to the manufacturer’s instructions. Following electrophoresis of the resulting digestion

(19) Comparing neoantigen cancer vaccines and immune checkpoint therapy unveils an effective vaccine and anti-TREM2 macrophage-targeting dual therapyCell ReportsNovember 26, 2024Sunita Keshari, Alexander S. Shavkunov, Qi Miao, Akata Saha, Tomoyuki Minowa, Martina Molgora, Charmelle D. Williams, Mehdi Chaib, Anna M. Highsmith, Josué E. Pineda, et al.vac-pic Gibson Assembly® Cloning Kit,New England Biolabs,Cat# E5510S BglII,New England Biolabs,Cat# R0144S "Mutant Lama4 peptide, sequence VGFNFRTL","Gubin et al.,16 Peptide 2.0",N/A "Mutant Alg8 peptide,

(20) SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular functionEMBO reportsNovember 1, 2024Ranjan G, Sehgal P, Scaria V, Sivasubbu S.(Tricaine),Sigma-Aldrich,E10521 TB Green Premix Ex Taq II (Tli RNase H Plus),Takara,RR82LR BglII,NEB,R0144S XhoI,NEB,R0146S Kpn1,NEB,R0142S MseI,NEB,R0525S Paraformaldehyde,Sigma-Aldrich,158127 DIG RNA Labeling Mix

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(1)

4/24/2018Diagnostic Restriction Mapping Of Plasmid Construct With BglII Restriction Enzme Triveni MahendrappaI have constructed a plasmid called pCVD442_operon B of a size 18992 bp size. This plasmid was prepped from carrier E. coli strains and digested with BglII restriction enzyme from NEB. Restriction ... Read Review
(1)

10/2/2015High Quality Restriction Enzyme for Cloning Prabhakaran KumarI used this BglII restriction enzyme along with EcoRV for the cloning of my gene of interest into a plasmid vector. Subsequently, I also used this enzyme for colony screening. This enzyme completely ... Read Review

BglII

BglII from New England Biolabs

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New England Biolabs

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BglII from New England Biolabs

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