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BglI
BglI
Supplier Images
BglI
BglI from New England Biolabs
Be the first to write a review!
Citations:
(17)
Supplier Page
Supplier Page from
New England Biolabs for
BglI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BglI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0143L
Concentration
10 u/µL
Quantity
10000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
GCCN4/NGGC
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (17)
(1)
RdJ detection tests to identify a unique MRSA clone of ST105-SCCmecII lineage and its variants disseminated in the metropolitan region of Rio de Janeiro
Frontiers in Microbiology
November 20, 2023
Matheus Assis Côrtes Esteves, Alice Slotfeldt Viana, Gabriela Nogueira Viçosa, Ana Maria Nunes Botelho, Ahmed M. Moustafa, Felipe Raposo Passos Mansoldo, Adriana Lucia Pires Ferreira, Alane Beatriz Vermelho, Bernadete Teixeira Ferreira-Carvalho, Paul Joseph Planet,
et al.
product was treated with 10 U of
BglI
and the reaction performed as recommended by the manufacturer (Cat#
R0143L
;
New England Biolabs
, Ipswich, MA, United States). After
incubation
at 37°C/1 h, 1 μL of 6 X Gel Loading
(2)
Several cell-intrinsic effectors drive type I interferon-mediated restriction of HIV-1 in primary CD4 + T cells
bioRxiv
February 12, 2023
Hannah L. Itell, Daryl Humes, Julie Overbaugh
PBS, and subjected to genomic DNA isolation (QIAGEN #
51104
). Genomic DNA was digested with
BglI
(
NEB
#
R0143L
) and diluted
1:3
for droplet
digital PCR
(ddPCR)
amplification
to
measure
vector copies per cell. Per
(3)
The rapid and highly parallel identification of antibodies with defined biological activities by SLISY
Nature communications
January 3, 2023
Lu S, Mattox AK, Aitana Azurmendi P, Christodoulou I, Wright KM, Popoli M, Chen Z, Sur S, Li Y, Bonifant CL,
et al
.
µM in TE Buffer. The pADL-10b phagemid vector was digested using
BglI
enzyme (
New England Biolabs
,
R0143S
) with
Antarctic Phosphatase
(
New England Biolabs
,
M0289L
) in manufacturer recommended Buffer 3.1 for
(4)
Protocol for high-throughput reservoir quantification across global HIV subtypes using a cross-subtype intact proviral DNA assay
STAR Protocols
December 1, 2022
Carolyn S. Fish, Noah A.J. Cassidy, Claire N. Levy, Sean M. Hughes, Keith R. Jerome, Julie Overbaugh, Florian Hladik, Dara A. Lehman
,
New England Biolabs
,Cat#
R0117S
ddPCR Supermix for probes (no dUTP
),
Bio-Rad
,Cat#
1863024
BgII,
New England Biolabs
,Cat#
R0143S
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,, J-Lat
(5)
HIV reservoir quantification using cross-subtype multiplex ddPCR
iScience
January 21, 2022
Noah A.J. Cassidy, Carolyn S. Fish, Claire N. Levy, Pavitra Roychoudhury, Daniel B. Reeves, Sean M. Hughes, Joshua T. Schiffer, Sarah Benki-Nugent, Grace John-Stewart, Dalton Wamalwa,
et al.
R0117S
ddPCR supermix for probes (no dUTP
),
Bio-Rad
,"Cat# 1,863,024" BgII,
New England BioLabs
,Cat#
R0143S
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,, J-lat
(6)
HIV reservoir quantification by five-target multiplex droplet digital PCR
STAR Protocols
December 17, 2021
Claire N. Levy, Sean M. Hughes, Pavitra Roychoudhury, Chelsea Amstuz, Haiying Zhu, Meei-Li Huang, Dara A. Lehman, Keith R. Jerome, Florian Hladik
min hands-on, 19 h
incubation
In this step, the restriction enzyme
BglI
(
New England Biolabs
, Cat#
R0143S
) is used to digest the high molecular weight nucleic acid isolated in the previous step. This enzyme
(7)
Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle
Journal of Cell Biology
December 6, 2021
Harriet P. Lo et al.
Sanger sequencing
(Australian Genome Research Facility, Brisbane) or
restriction digest
with
BglI
(#
R0143S
;
NEB
). TILLING (targeting induced local lesions in genomes)
mutagenesis
screen for identification of
(8)
A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir
Cell Reports Medicine
April 20, 2021
Claire N. Levy et al.
,
New England BioLabs
,Cat#
R0560S
HindIII
,
New England BioLabs
,Cat#
R0104S
ScaI
,
New England BioLabs
,Cat#
R3122S
BglI
,
New England BioLabs
,Cat#
R0143S
"Proteinase K, 20mg/mL, > 318mAu/mL at 30°C",
QIAGEN
,Cat#
19131
AatII
,
New England BioLabs
,Cat#
R0117S
Pen
(9)
Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening
Nature communications
March 12, 2021
Baxley RM, Leung W, Schmit MM, Matson JP, Yin L, Oram MK, Wang L, Taylor J, Hedberg J, Rogers CB,
et al
.
R0622);
CDC45
exon 3,
PflMI
(
NEB
R0509
),
XcmI
(
NEB
R0533
) or
AlwNI
(
NEB
R0514
);
MCM4
exon 2,
BglI
(
NEB
R0143
). To generate HCT116 MUS81-/- exon 2 mutant cell lines, each parental line was
transfected
with 1 µL
(10)
Arsenic Metabolism in Mice Carrying a
BORCS7/AS3MT
Locus Humanized by Syntenic Replacement
Environmental Health Perspectives
August 12, 2020
Beverly H. Koller, John N. Snouwaert, Christelle Douillet, Leigh A. Jania, Hisham El-Masri, David J. Thomas, Miroslav Stýblo
described above, and the Black-Red and Purple homology arm PCRs were digested with
BglI
(
NEB
; Cat. no.
R0143S
) and then column purified as described above. The loxP-flanked neomycin resistance gene was ligated
(11)
Delta glutamate receptor conductance drives excitation of mouse dorsal raphe neurons
eLife
April 1, 2020
Gantz SC, Moussawi K, Hake HS.
Addgene 131682",Lot# AAV746 "Chemical compound, drug",Bgl I restriction enzyme,
New England Biolabs
,Cat#
R0143S
,- "Chemical compound, drug",D-serine,
Millipore Sigma
,Cat#
S4250
,10 mM "Chemical compound, drug",GDPβS-Li3,
Millipore Sigma
(12)
High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier
eLife
July 31, 2019
Zhijie Chen, Ronen Gabizon, Aidan I Brown, Antony Lee, Aixin Song, César Díaz-Celis, Craig D Kaplan, Elena F Koslover, Tingting Yao, Carlos Bustamante
kit,
DraIII-HF
,
NEB
,Cat#
R3510S
, Commercial
assay
or kit,
BsaI-HF
,
NEB
,Cat#
R3535L
, Commercial
assay
or kit,
BglI
,
NEB
,Cat#
R0143S
, Commercial
assay
or kit,
EagI-HF
,
NEB
,Cat#
R3505S
, Commercial
assay
or kit,
SapI
,
NEB
,Cat#
R0569S
, Sequence-based
(13)
YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo
Developmental cell
March 25, 2019
Monroe TO, Hill MC, Morikawa Y, Leach JP, Heallen T, Cao S, Krijger PHL, de Laat W, Wehrens XHT, Rodney GG,
et al
.
A,Sigma-Aldrich,Cat#10103586001
Blebbistatin
,
Sigma-Aldrich
,Cat#
B0560
di-4-ANEPS,Invitrogen,Cat#D-1199
BglII
,
NEB
,Cat#
R0143S
EcoRI-HF
®,
NEB
,Cat#
R3101
BsrGI-HF
®,
NEB
,Cat#
R3575L
T4 DNA ligase
(3C),
NEB
,Cat#
R3575L
Proteinase K
,
Sigma-Aldrich
,Cat#
P2308
-25MG RNAse
(14)
mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels
EMBO molecular medicine
September 1, 2018
Pereira CV, Bacman SR, Arguello T, Zekonyte U, Williams SL, Edgell DR, Moraes CT.
R‐GTAGTATTTTAGTTTGGGGCATTTCACTGTAAAGCCGTGTTGG (Hashimoto et al, 2015). Therefore,
PCR
products were digested with
BglI
(
R0143S
,
New England Biolabs
) in
NEBuffer 3.1
(
B7203S
,
New England Biolabs
), for 4 h at 37°C (Hashimoto et al,
(15)
Short-Term Fasting Reveals Amino Acid Metabolism as a Major Sex-Discriminating Factor in the Liver
Cell Metabolism
August 7, 2018
Sara Della Torre, Nico Mitro, Clara Meda, Federica Lolli, Silvia Pedretti, Matteo Barcella, Luisa Ottobrini, Daniel Metzger, Donatella Caruso, Adriana Maggi
UNG,ThermoFisher/
Life Technologies
,
4324018
PshAI
restriction enzyme,
NEB
,
R0593
BglI
restriction enzyme,
NEB
,
R0143
G 418 disulfate salt
,
Sigma Aldrich
,
A1720
,, Critical Commercial
Assays
,Critical Commercial
Assays
,Critical
(16)
Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo
Developmental Biology
January 27, 2017
Weibin Zhang, Sudipto Roy
(17)
Analysis of DNA methylation acquisition at the imprinted Dlk1 locus reveals asymmetry at CpG dyads
Epigenetics & Chromatin
December 1, 2014
Alyssa Gagne, Abigail Hochman, Mahvish Qureshi, Celia Tong, Jessica Arbon, Kayla McDaniel, Tamara L Davis
prior to bisulfite
mutagenesis
as follows: 0.5 μg of genomic DNA was digested with 1 μL
BglI
(
NEB
, cat#
R0143S
) and ligated to 1 μg of a phosphorylated hairpin linker (5′-AGCGATGCGTTCGAGCATCGCTCCC-3′) [35]. A total
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BglI from New England Biolabs
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