BfuAI from New England Biolabs

Product Specs
ItemBfuAI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0701S
Quantity250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Evaluation of Cas13d as a tool for genetic interaction mappingNat CommunFebruary 14, 2025Ghanem El Kassem, Jasmine Hillmer, Michael BoettcherNucleoSpin columns (Macherey-Nagel, 740609.250). The 783-Rx-hU6 plasmid was digested with BfuAI (NEB, R0701S) and purified from 1% agarose gel. The single gRNAs and arrays were cloned into the digested 783-Rx-hU6

(2) IRF4 requires ARID1A to establish plasma cell identity in multiple myelomaCancer CellJuly 8, 2024Arnold Bolomsky, Michele Ceribelli, Sebastian Scheich, Kristina Rinaldi, Da Wei Huang, Papiya Chakraborty, Lisette Pham, George W. Wright, Tony Hsiao, Vivian Morris, et al.Cocktail",SigmaAldrich,Cat.#11836170001 Ficoll-Paque PLUS density gradient media,Cytiva,Cat.#17144002 BfuA1,New England Biolabs,Cat.#R0701L SnaB1,New England Biolabs,Cat.#R0130L Puromycin Dihydrochloride,Thermo Fisher Scientific,Cat.#A1113802 Geneticin™

(3) Genetically defined nucleus incertus neurons differ in connectivity and functionElifeMay 31, 2024Emma D Spikol, Ji Cheng, Michelle Macurak, Abhignya Subedi, Marnie E Halpernannealed to each other. The pPRISM-QF2-he1.1:YFP donor vector was first digested with BfuAI and BspQI, (R0701S and R0712S, New England Biolabs) and then combined with the homology arms in a ligation reaction (M0202S,

(4) Response to Bruton’s tyrosine kinase inhibitors in aggressive lymphomas linked to chronic selective autophagyCancer CellFebruary 1, 2024James D. Phelan, Sebastian Scheich, Jaewoo Choi, George W. Wright, Björn Häupl, Ryan M. Young, Sara A. Rieke, Martine Pape, Yanlong Ji, Henning Urlaub, et al.Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent,Thermo Fisher,Cat.#22663 4x Laemmli sample buffer,BioRad,Cat #1610747 BfuA1,New England Biolabs,Cat.#R0701L SnaB1,New England Biolabs,Cat.#R0130L Puromycin Dihydrochloride,Thermo Fisher Scientific,Cat.#A1113802 Geneticin™

(5) X-CHIME enables combinatorial, inducible, lineage-specific, and sequential knockout of genes in the immune systemNature ImmunologyJanuary 1, 2024Martin W. LaFleur, Ashlyn M. Lemmen, Ivy S. L. Streeter, Thao H. Nguyen, Lauren E. Milling, Nicole M. Derosia, Zachary M. Hoffman, Jacob E. Gillis, Qin Tjokrosurjo, Samuel C. Markson, et al.knockout studies were cloned into our lentiviral vectors using BsmBI (NEB Cat# R0580L) and BfuAI (NEB Cat# R0701L) restriction digests. Plasmids were amplified in Stbl3 bacteria (Invitrogen Cat# C737303) and extracted

(6) A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem cellsSTAR protocolsJune 17, 2022Nair VP, Mayer J, Vincendeau M.recombinant proteins","Chemicals, peptides and recombinant proteins" ,, BfuA1,New England BioLabs,R0701S Tris-EDTA,"Sigma-Aldrich, Darmstadt, Germany",93283 T4 DNA ligase,New England BioLabs,EL0011 Ampicillin,"Sigma-Aldrich,

(7) Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3Cell stem cellSeptember 2, 2021Padmanabhan Nair V, Liu H, Ciceri G, Jungverdorben J, Frishman G, Tchieu J, Cederquist GY, Rothenaigner I, Schorpp K, Klepper L, et al.and cooled to room temperature o/n. 2μg of the vector was digested using 2 units of BfuA1 enzyme (#R0701S, NEB) at 50°C for 1.5 hours. The enzyme was inactivated at 65°C for 30 minutes. After ensuring complete

(8) Inhibition of mitochondrial translation suppresses glioblastoma stem cell growthCell reportsApril 27, 2021Sighel D, Notarangelo M, Aibara S, Re A, Ricci G, Guida M, Soldano A, Adami V, Ambrosini C, Broso F, et al.Louis Staudt, National Cancer Institute, USA). The resulting plasmid was digested with BfuAI (NEB cat. R0701S). Complementary sgRNA sequences flanked by ACCG on the 5′ end, and CTTT on the 3′ of the reverse strand

(9) Genome-wide Screens Identify Lineage- and Tumor-Specific Genes Modulating MHC-I- and MHC-II-Restricted Immunosurveillance of Human LymphomasImmunityJanuary 12, 2021Dersh D, Phelan JD, Gumina ME, Wang B, Arbuckle JH, Holly J, Kishton RJ, Markowitz TE, Seedhom MO, Fridlyand N, et al.927-70001 Dynabeads Protein G magnetic,Invitrogen,Cat# 10003D DMSO,ATCC,Cat# 4-X-5 BfuAI,New England Biolabs,Cat# R0701L Antarctic Phosphatase,New England Biolabs,Cat# M0289S T4 PNK,New England Biolabs,Cat# M0201S T4 DNA ligase

(10) A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome EditingMolecular cellFebruary 21, 2019Edraki A, Mir A, Ibraheim R, Gainetdinov I, Yoon Y, Song CQ, Cao Y, Gallant J, Xue W, Rivera-Pérez JA, et al.inhibitor",Sigma-Aldrich,Cat#11836170001 T4 DNA ligase,NEB,Cat#M0202 T4 PNK,NEB,Cat#M0201 BfuAI,NEB,Cat#R0701 Critical Commercial Assays,, ,, ,, ,, ,, ,, Deposited Data,, ,, NCBI BioSample database,,SAMN09694825 Mendeley

(11) Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune FunctionCellDecember 13, 2018Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A.#52963) with flanking BfuAI cut sites. This stuffer was excised using the BfuAI restriction enzyme (NEB, #R0701) and the linear backbone was gel purified (Zymo, #D4007). We designed a targeted library to include

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BfuAI

BfuAI from New England Biolabs

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New England Biolabs

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BfuAI from New England Biolabs

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