BciVI from New England Biolabs

Product Specs
ItemBciVI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0596S
Concentration10 u/µL
Quantity200 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-80 C

Sequence

GTATCC(6/5)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissueCellAugust 21, 2025Reuben A. Saunders, William E. Allen, Xingjie Pan, Jaspreet Sandhu, Jiaqi Lu, Thomas K. Lau, Karina Smolyar, Zuri A. Sullivan, Catherine Dulac, Jonathan S. Weissman, et al.Mix,NEB,Cat# N0447S Aminoallyl-dUTP,ThermoFisher,Cat# R1101 BccI,NEB,Cat# R0704S BciVI,NEB,Cat# R0596S NaCl,Ambion,Cat# AM9759 MgCl2,Ambion,Cat# AM9530G TE,Ambion,Cat# AM9849 Invitrogen™ SiteClick™ Antibody

(2) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsGenome BiolMay 25, 2025Joseph H. Lobel, Nicholas T. Ingoliaplasmids containing the reporters and promoters (pJL206 or pJL261) were linearized with BciVI (NEB, R0596S) for 1 h at 37 °C. The digestions were purified by spin column (Qiagen, 28104) according to the manufacturer’s

(3) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsbioRxivSeptember 10, 2024Joseph H. Lobel, Nicholas T. Ingoliaplasmids containing the reporters and promoters (pJL206 or pJL261) were linearized with BciVI (NEB, R0596S) for 1 h at 37 °C. The digestions were purified by spin column (Qiagen, 28104) according to the manufacturer’s

(4) Mutational scanning pinpoints distinct binding sites of key ATGL regulators in lipolysisNat CommunMarch 21, 2024Johanna M. Kohlmayr, Gernot F. Grabner, Anna Nusser, Anna Höll, Verina Manojlović, Bettina Halwachs, Sarah Masser, Evelyne Jany-Luig, Hanna Engelke, Robert Zimmermann, et al.via PCR and adapter sequences were removed using restriction enzymes BciVI (New England BioLabs GmbH, R0596S) and BspQI (New England BioLabs GmbH, R0712S). Purified via an 8% acrylamide 8 M urea gel. Bands of

(5) Shortcut barcoding and early pooling for scalable multiplex single-cell reduced-representation CpG methylation sequencing at single nucleotide resolutionNucleic Acids ResearchNovember 27, 2023Liyao Mai, Zebin Wen, Yulong Zhang, Yu Gao, Guanchuan Lin, Zhiwei Lian, Xiang Yang, Jingjing Zhou, Xianwei Lin, Chaochao Luo, et al.solution was incubated with 3 μl digestion mixture containing 1 μl BciVI (New England Biolabs, cat. no. R0596S) and 2 μl CutSmart buffer (New England Biolabs, cat. no. B7204S, 10×) at 37°C for 2h, followed by heating

(6) Reusable Single Cell for Iterative Epigenomic AnalysesJournal of Visualized ExperimentsFebruary 28, 2022Hidetaka Ohnuki, David J. Venzon, Alexei Lobanov, Giovanna Tosato[4H8],Abcam,ab5408,Anti-Pol II Anti-TRAP220/MED1 (phospho T1457) antibody,Abcam,ab60950,Anti-Med1 BciVI,New England BioLabs,R0596L,BciVI "Bovine Serum Albumin solution, 20 mg/mL in H2O, low bioburden, protease-free, for molecular

(7) The ubiquitin ligase RFWD3 is required for translesion DNA synthesisMolecular cellFebruary 4, 2021Gallina I, Hendriks IA, Hoffmann S, Larsen NB, Johansen J, Colding-Christensen CS, Schubert L, Sellés-Baiget S, Fábián Z, Kühbacher U, et al.restriction enzyme,New England BioLabs,Cat#R0103S SapI restriction enzyme,New England BioLabs,Cat#R0596S "Proteinase K, recombinant",Roche,Cat#3115879001 SYBR® Gold Nucleic Acid Gel Stain,Thermo Fisher,Cat#S11494 Phusion® High-Fidelity DNA Polymerase

(8) A standard for near-scarless plasmid construction using reusable DNA partsNature CommunicationsDecember 1, 2019Xiaoqiang Ma, Hong Liang, Xiaoyi Cui, Yurou Liu, Hongyuan Lu, Wenbo Ning, Nga Yu Poon, Benjamin Ho, Kang Zhouligation solution contained 1 µL of Type IIS RE (MboII [R0148S], HphI [R0158S], BmrI [R0600S] or BciVI [R0596S] NEB), 0.3 µL of 1.25 μM L(G/A)-Boligo, 0.3 µL of 1.25 μM R(G/A)-Boligo, 3 µL of fragment (the concentration

(9) Single-cell ChIP-seq reveals cell subpopulations defined by chromatin stateNature biotechnologyNovember 1, 2015Rotem A, Ram O, Shoresh N, Sperling RA, Goren A, Weitz DA, Bernstein BE.at 37C for 30 minutes. We then purify the DNA using 1.1× AMPure XP beads, add 1uL of BciVi enzyme (R0596L, NEB, USA) and 2.5ul of NEB Buffer 4 in a total volume of 25uL including the DNA and incubate at 37C

(10) Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeqNature ProtocolsSeptember 1, 2015James Ding, Martin S. Taylor, Andrew P. Jackson, Martin A. M. Reijns(New England Biolabs, cat. no. R0707) BciVI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0596) Shrimp Alkaline Phosphatase (SAP), supplied with 10x reaction buffer (Affymetrix USB®, cat. no. 70092Z)

(11) Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seqNature ProtocolsOctober 1, 2011Mazhar Adli, Bradley E. Bernsteinbuffer) Primer 2 (10 μM stock): 5'-GACATGTATCCGGATGT-3’ BciVI Restriction Enzyme (NEB, Cat. #: R0596L) Agarose (Fisher, Cat #: BP160-500) Protein A-sepharose beads (Sigma, Cat#: P9424) PCR purification,

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BciVI

BciVI from New England Biolabs

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BciVI from New England Biolabs

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