BbsI from New England Biolabs

Product Specs
ItemBbsI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0539S
Quantity300 units
ApplicationsDNA Manipulation
Concentration5 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAAGAC(2/6)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activatorNucleic Acids ResApril 25, 2026María Mariner-Faulí, Víctor Sánchez-Gaya, Sarah Malika Robert, Patricia Respuela, Sara de la Cruz-Molina, Sara Lobato-Moreno, Matteo Trovato, Karin D Prummel, Kyung-Min Noh, Judith B Zaugg, et al.on-target efficiency and minimal off-target potential. Complementary oligonucleotides containing BbsI (NEB, R0539L) overhangs were synthesized and annealed (see Supplementary Data 4) by heating to 95°C for 5 min, followed

(2) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.95°C, 50 min → ramp down to 25°C at 5°C/min. XbaI or Bbs1 digestion (New England Biolabs Cat# R0145S or R0539S), DNA gel extraction (QIAGEN Cat# 28704), and ligation (New England Biolabs Cat# M0202S) were conducted

(3) Protocol for Using CRISPR-Cas9 to Generate a Monocyte Cell Line Harboring a Single-Nucleotide PolymorphismBIO-PROTOCOLApril 20, 2026Amar Chandra Mahatha, Octavio Ramos-Espinosa, Dhemerson Souza De Lima, Ethan Y. Liu, Sarah Vaidyan, Jianguo Liuligase master mix (Invitrogen, catalog number: IVGN2102) 2. BbsI (New England BioLabs, catalog number: R0539S) 3. T4 polynucleotide kinase (PNK) (Thermoscientific, catalog number: EK0031) 4. rCutSmart buffer, 10×

(4) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik NiopekIIs enzymes used included Esp3I (New England Biolabs, cat. no. R0734S), BsaI-HF®v2 (R3733S), BbsI (R0539S), SapI (R0569S), and PaqCI® (R0745S). Plasmids were assembled via Golden Gate cloning. Plasmids were

(5) COG5 deficiency disrupts cellular copper homeostasis and underlies the impaired mitochondrial OXPHOS functionPLoS GenetMarch 13, 2026Yuwei Zhou, Keyi Li, Ruowei Zhu, Xue Ma, Xinfei Ye, Mengqing Mao, Ding Li, Xiaofei Zeng, Zhehui Chen, Jing Wu, et al.pX330 (RRID: Addgene_42230) and digested by restriction enzyme Bbsl (New England Biolabs, USA, Cat#R0539) to generate the COG5-targeted KO vectors. KO cells (KO1 and KO2) and control cells were obtained by

(6) TDG orchestrates ATF4-dependent gene transcription during retinoic acid-induced cell fate acquisitionNucleic Acids ResMarch 3, 2026Marion Turpin, Thierry Madigou, Maud Bizot, Rachael Acker, Stephane Avner, Gérard Benoît, Martin Braud, Cynthia Fourgeux, Gaëlle Palierne, Jeremie Poschmann, et al.(http://crispor.tefor.net/) and introduced either into pX458 or pX459 (one gRNA, Addgene) at the BbsI (NEB, R0539) site. Wt ECCs were seeded into P100 Petri dishes and transfected for 24 h with vectors containing the

(7) HDAC5 -encoded Microprotein NISM Mediates Nucleolar Formation and Ribosomal RNA SynthesisbioRxivFebruary 25, 2026Kevin Cao, Dat Ha, Jesse Hulahan, Lilianna Houston, Gregory Tong, Jiayi Weng, Natasha Huey, Hannah E. DeMerit, Pedro Ortega, Rémi Buisson, et al.pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene, 62988) using the BbsI restriction enzyme (New England Biolabs, R0539) and T4 DNA ligase (New England Biolabs, M0202). Ligated constructs were transformed into high efficiency

(8) A genome-scale single-cell CRISPRi map of trans gene regulation across human pluripotent stem cell linesCell GenomicsFebruary 11, 2026Claudia Feng, Elin Madli Peets, Yan Zhou, Luca Crepaldi, Sunay Usluer, Alistair Dunham, Jana M. Braunger, Jing Su, Magdalena E. Strauss, Daniele Muraro, et al.proteins","Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, BbsI,NEB,R0539L NotI,NEB,R0189S EcoRV-HF,NEB,R3195S ,, Critical commercial assays,Critical commercial assays,Critical

(9) GPR39 activation inhibits AQP2 trafficking and alters cytoskeletal organizationAmerican Journal of Physiology-Cell PhysiologyFebruary 1, 2026Mackenzie K. Kui, Jessica J. Zhang, Ibrahim A. Ahmed, Shishir Kumar Patel, Tara Fallah Fallah Rastegar, Hamid Rabb, Jennifer L. PluznickLab, mpkCCD GPR39 KO,This paper, Cloning,, px458-GFP-mGPR39-gRNA2,This paper, BbsI restriction enzyme,NEB,R0539S

(10) Plasmodium berghei High-Throughput (PbHiT): a CRISPR-Cas9 System to Study Genes at ScaleBIO-PROTOCOLJanuary 20, 2026Thorey K. Jonsdottir, Martina S. Paoletta, Johan Henriksson, Ellen S.C. Bushell(New England Biolabs, catalog number: R0140L) 5. BbsI restriction enzyme (New England Biolabs, catalog number: R0539L) 6. AvrII restriction enzyme (New England Biolabs, catalog number: R0174L) 7. TAE buffer, 50× (VWR,

(11) Site-saturation functional screens identify PALB2 missense variants associated with increased breast cancer riskNature CommunicationsJanuary 19, 2026Rick A.C.M. Boonen, Sabine C. Knaup, Roberta Menafra, Merel E. Braspenning, Magdalena B. Rother, Petra Kleiblova, Marketa Janatova, Dina Ruano, Chunling Hu, Huaizhi Huang, et al.ligated into pSpCas9(BB)-2A-Puro (PX459 v2.0) (Addgene; 62988) following BbsI (New England Biolabs, R0539L) digestion to create Cas9-sgRNA co-expression construct for SGE. For the SGE, 600-1000 bp homologous

(12) Genetic evidence for PARP1 trapping as a driver of PARP inhibitor efficacy in BRCA mutant cancer cellsNucleic Acids ResDecember 11, 2025Jonathan Ribeiro, Kjetil Hansen, Lotte van Beek, Christopher Stubbs, Joan Frigola, Sara Talbot, Sabrina Bentouati, James Hall, Paul W G Wijnhoven, Josh Armenia, et al.annealed and then inserted into the pKLV2-U6gRNA(Bbsl)-PCKpuro2AZsG-W plasmid digested with the Bbs1 (#R0539S, New England Biolabs) restriction enzyme. Lentiviruses were generated by transfecting (Lipofectamine

(13) Sirtuin 6 is a histone delactylaseJournal of Biological ChemistryDecember 1, 2025Garrison A. Nickel, Nicholas J. Pederson, Faheem, Zhenyu Yang, Jack Bulf, Katharine L. DiehlQ5 High-Fidelity DNA Polymerase,New England Biolabs (NEB),M0491 Dpn1,New England Biolabs (NEB),R0176 Bbs1,New England Biolabs (NEB),R0539 T4 PNK,New England Biolabs (NEB),M0201 T4 DNA ligase,New England Biolabs (NEB),M0202 Micrococcal Nuclease

(14) Regulation of the Homeostasis of Early Embryo Development in Dairy Cows by Targeted Editing of the PRLR Gene-Mediated Activation of the Anti-Heat Stress PathwayCellsNovember 25, 2025Xin Cheng, Daqing Wang, Xingyu Zhang, Lu Li, Yiyi Liu, Guifang Cao, Yong Zhangusing T7 endonuclease I (T7EI, TransGen Biotech, Beijing, China) and Bbs I restriction endonuclease (R0539S, New England Biolabs, Ipswich, MA, USA). Reactive oxygen species (ROS) levels were assessed using the

(15) A Chemically Induced CRISPR/dCas13 FCPF Platform for Precise and Programmable RNA RegulationJournal of Medicinal ChemistryNovember 13, 2025Sebastian Hasselbeck, Jianhui Wang, Zhaodai Bai, Tobias Hüfner, Gerhard Hummer, Phillip Grote, Xinlai ChengFor Cas13 crRNA expression, plasmid construction from LwaCas13a, RanCas13b, and PspCas13b, BbsI (NEB, R0539L) was used for the digestion of pC0040, pC0041, and pC0043. For Cas13 gRNA expression, plasmid construction

(16) Mammalian and Bacterial Adaptors Function as Co-Disinhibitory Pairs to Activate the E3 ubiquitin ligase WWP2Journal of Biological ChemistryOctober 22, 2025Samkeliso V. Blundell, Mei Liu, Romina Tocci, Andrea Majstorovic, Solène Tsang, David W. Holdenreagents","Chemicals, enzymes, and other reagents" DpnI,NEB,R0176L BsmBI-V2,NEB,R0739S BbsI,NEB,R0539S HindIII-HF,NEB,R3104S NotI- HF,NEB,R3189S NcoI-HF,NEB,R3193S Foetal calf serum,"Gibco, Life Technologies",10082147 Dulbecco’s

(17) Polycomb Repressive Complex 1 and USP16 localize to the mitochondrion and influence its functionProceedings of the National Academy of SciencesOctober 21, 2025Lei Shi, Chunxu Chen, Xiaokun Jian, Qiushi Wang, Yuan Shen, Asmaa Alajmi, Hao Zhang, Lisa S. Shock, Louise T. Chow, Hengbin Wang(Addgene #127763), with the sgRNA sequence 5′-GTGCGTAATAAAGTTCCAT-3′ cloned into the BbsI site (NEB, R0539S). The repair template contained left and right homology arms amplified from HEK293T genome DNA template

(18) Generation of Insulin-Producing Alpha TC1-6 Cells Using EpiCRISPR System for Targeted DNA MethylationBio ProtocOctober 20, 2025Marija B. Đorđević, Stefan Marković Ž. Hadžić, Jovana J. Rajić, Ana D. Sarić, Tijana R. Marković, Svetlana S. Dinić, Aleksandra S. Uskoković, Nevena M. Grdović, Mirjana V. Mihailović, Jelena Arambašić D. Jovanović, et al.(Thermo Fisher Scientific, catalog number: B69) (-20 °C) 23. BbsI enzyme (New England Biolabs, U.S.A, catalog number: R0539S) (-20 °C) Solutions 1. DMEM high glucose full medium (see Recipes) 2. DMEM low glucose full medium (see

(19) An AI-driven workflow for the accelerated optimization of cell-free protein synthesisiScienceOctober 17, 2025Mostafa M. Khalil, Aisha Elsawah, An N. Hoang, Jean-Loup Faulon, Baptiste Panthu, Joan HérissonL-Phenylalanine,Sigma,Cat#78019 Amino acids - lsoleucine,Sigma,Cat#I7403 BsmBI-v2,NEB,Cat#R0739S BbsI,NEB,Cat#R0539S BsaI,NEB,Cat#R3733S DpnI,NEB,Cat#R0176L BamHI,Promega,Cat#R6021 EcoRV,Promega,Cat#R6351 T4 DNA ligase

(20) NAA60 facilitates LRRC8A- and LRRC8D-mediated platinum drug uptakeCommunications biologyOctober 6, 2025Widmer CA, Moyseos A, Klebic I, Dettwiler M, González-Fernández M, Gogola E, Siffert M, Buchs N, Braga-Lagache S, Uldry AC, et al.annealed at RT for 2 h and subsequently ligated with quick-ligase (NEB Cat#M2200S) into BbsI (NEB Cat#R0539) digested pX330 or BsmBI-digested (Fermantas Cat#FD0454) lentiCRISPRv2 backbone. Sanger sequencing verified

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BbsI

BbsI from New England Biolabs

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BbsI from New England Biolabs

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