Cultrex 3-D Culture Matrix RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex 3-D Culture Matrix RGF Basement Membrane Extract
CompanyR&D Systems, a Bio-Techne Brand
Price $290.00Supplier Page View Company Product Page
Catalog Number3445-005-01
Quantity5 mL
SpeciesMulti-Species
ApplicationsBioactivity
Format14 - 16 mg/mL
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 µg/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines

Specifications/Features

Available conjugates: Sizes Available: 5 mL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) DNA damaging agents boost the transcription of endothelin A receptor in high-grade serous ovarian cancerJournal of Experimental & Clinical Cancer ResearchDecember 6, 2025Celia Roman, Rosanna Sestito, Valentina Caprara, Andrea Sacconi, Giovanni Blandino, Anna Bagnato, Piera Toccithe Boyden Chamber (8 μm pore size; Greiner Bio-One) pre-coated with Cultrex Culture Matrix (cat. no. 3445-005−01; R&D Systems) and medium containing 10% FBS was added to the lower chamber. After 48 h of treatment

(2) Impact of conduit-filling interactions on the efficacy of fiber and hydrogel fillers in nerve conduitsiScienceAugust 15, 2025Flavia Millesi, Sascha Mero, Sebastian Rihl, Sophie Steinwenter, Sarah Stadlmayr, Anton Borger, Paul Supper, Maximilian Haertinger, Leon Ploszczanski, Gerhard Sinn, et al.Guide,Integra,PNG230 Reaxon®,Kerimedical,RD121 Cultrex™ Basement Membrane Extract 3D Cell Culture Matrix,R&D Systems,3445-005-01 μ-slide 8-well chamber slides,ibidi,80826 Cantilevers NSG03,NT-MDT,NSG03

(3) Calcium levels modulate platelet function, platelet-cancer cell interaction, and cancer cell invasionSci RepMarch 5, 2025Kenise Morris, Salah Masri, Brian Schnoor, Anne-Laure PapaScienCell Research Laboratories. Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) (cat# 3445-005-01) was purchased from Bio-techne. EDTA (10% w/v) (cat# 2670-4) and sodium citrate (10% w/v) (cat# 723016)

(4) The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fatebioRxiv : the preprint server for biologyJuly 11, 2024Marin H, Simental E, Allen C, Martin E, Panning B, Al-Sady B, Buchwalter A.was coated with 1 mL of a 1:200 solution of either Geltrex (Gibco™ A1413202) or Cultrex (Bio-Techne 3445-005-01) in N2B27 at 37°C. After one hour, that solution was aspirated and 2 × 105 mESCs were seeded (approximately

(5) Prostate Cancer-Specific Lysine 53 Acetylation of Cytochrome c Drives Metabolic Reprogramming and Protects from Apoptosis in Intact CellsBiomoleculesJune 14, 2024Paul T. Morse, Junmei Wan, Tasnim Arroum, Mackenzie K. Herroon, Hasini A. Kalpage, Viktoriia Bazylianska, Icksoo Lee, Elisabeth I. Heath, Izabela Podgorski, Maik Hüttemann(#430165, Thermo Fisher Scientific; Waltham, MA, USA), and 45 µL of non-diluted (~15 mg/mL) Cultrex (#3445-005-01, R&D Systems; Minneapolis, MN, USA) was gently added on top of each coverslip. The dishes were incubated

(6) HIF2α-dependent Dock4/Rac1-signaling regulates formation of adherens junctions and cell polarity in normoxiaScientific reportsMay 27, 2024Raykhel I, Ronkainen VP, Myllyharju J, Manninen A.

(7) Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture systemSTAR ProtocolsMarch 15, 2024Jason A. Rizo, Thomas E. Spencer, Andrew M. Kelleher17075029 DNase-I,Roche,Cat# 10104159001 MgCl2 (1 M),Invitrogen,Cat# AM9530G Cultrex BME,R&D Systems,Cat# 3445-005-01 Dimethyl sulfoxide (DMSO),Fisher,Cat# D2650 Fetal bovine serum,Sigma,Cat# D1391 Collagenase V,Sigma,Cat#

(8) Therapeutic effect and transcriptome-methylome characteristics of METTL3 inhibition in liver hepatocellular carcinomaCancer cell internationalNovember 27, 2023Liu Q, Qi J, Li W, Tian X, Zhang J, Liu F, Lu X, Zang H, Liu C, Ma C, et al.HepG2 cells were seeded as 1 × 104 cells/well in DMEM medium containing 1 mg/ml Cultrex matrix (R&D systems, 3445–005-01) and cultured for 5 days to allow spheroid formation. A serial dilution of STM2457 (DMSO solvent control,

(9) Clonal cooperation through soluble metabolite exchange facilitates metastatic outgrowth by modulating Allee effectScience advancesSeptember 15, 2023Hershey BJ, Barozzi S, Orsenigo F, Pompei S, Iannelli F, Kamrad S, Matafora V, Pisati F, Calabrese L, Fragale G, et al.

(10) Estrogen receptor alpha regulates uterine epithelial lineage specification and homeostasisiScienceSeptember 15, 2023Rizo JA, Davenport KM, Winuthayanon W, Spencer TE, Kelleher AM.another round of centrifugation, the epithelium pellet was resuspended in 80% Cultrex (R&D Systems, 3445-005-01) and 20% organoid expansion media (Table S3). EEO were passaged (1:3 ratio) every 7-10 days. To passage

(11) Linear ubiquitination of PTEN impairs its function to promote prostate cancer progressionOncogeneOctober 1, 2022Guo Y, He J, Zhang H, Chen R, Li L, Liu X, Huang C, Qiang Z, Zhou Z, Wang Y, et al.

(12) Implanted synthetic cells trigger tissue angiogenesis through de novo production of recombinant growth factorsProceedings of the National Academy of Sciences of the United States of AmericaSeptember 20, 2022Chen G, Levin R, Landau S, Kaduri M, Adir O, Ianovici I, Krinsky N, Doppelt-Flikshtain O, Shklover J, Shainsky-Roitman J, et al.were seeded at 10,000 cells/well on top of a growth factor–reduced basement membrane extract (GFR BME; 3445–005-01, R&D Systems)–coated Angiogenesis μ-Slide (Ibidi). The cells were applied with the same treatments mentioned

(13) Organ-Specific, Fibroblast-Derived Matrix as a Tool for Studying Breast Cancer MetastasisCancersJuly 2, 2021Jensen ARD, Horton ER, Blicher LH, Pietras EJ, Steinhauer C, Reuten R, Schoof EM, Erler JT.in 35 mm plastic dishes. Dishes were also coated with Matrigel (Cultrex 3-D culture matrix cat. no. 3445-005-01, lot no. 1519214, R&D Systems, MN, USA), which was diluted in serum-free media (1:1000) to obtain 15

(14) Limited inhibition of multiple nodes in a driver network blocks metastasiseLifeMay 11, 2021Yesilkanal AE, Yang D, Valdespino A, Tiwari P, Sabino AU, Nguyen LC, Lee J, Xie XH, Sun S, Dann C, et al.dye other,"BME (3-D Culture Matrix Reduced Growth Factor Basement Membrane Extract, PathClear)",Trevigen,3445-005-01,"Lot No 37353J16, lot concentration: 15.51 mg/ml" "peptide, recombinant protein",EGF (human),Bio-Techne,236-EG-01M, "software,

(15) Sprouty4 negatively regulates ERK/MAPK signaling and the transition from in situ to invasive breast ductal carcinomaPloS oneJanuary 1, 2021Brock EJ, Jackson RM, Boerner JL, Li Q, Tennis MA, Sloane BF, Mattingly RR.

(16) A new level of in vivo singlet molecular oxygen luminescence measurementsPhotodiagnosis and photodynamic therapyMarch 1, 2020Pfitzner M, Preuß A, Röder B.

(17) Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid cultureNature communicationsDecember 11, 2019Giobbe GG, Crowley C, Luni C, Campinoti S, Khedr M, Kretzschmar K, De Santis MM, Zambaiti E, Michielin F, Meran L, et al.

(18) Natural Histogel-Based Bio-Scaffolds for Sustaining Angiogenesis in Beige Adipose TissueCellsNovember 18, 2019Di Somma M, Schaafsma W, Grillo E, Vliora M, Dakou E, Corsini M, Ravelli C, Ronca R, Sakellariou P, Vanparijs J, et al.

(19) Smac mimetics LCL161 and GDC-0152 inhibit osteosarcoma growth and metastasis in miceBMC cancerSeptember 14, 2019Shekhar TM, Burvenich IJG, Harris MA, Rigopoulos A, Zanker D, Spurling A, Parker BS, Walkley CR, Scott AM, Hawkins CJ.

(20) Human mesenchymal stem cells secrete hyaluronan-coated extracellular vesiclesMatrix biology : journal of the International Society for Matrix BiologyDecember 1, 2017Arasu UT, Kärnä R, Härkönen K, Oikari S, Koistinen A, Kröger H, Qu C, Lammi MJ, Rilla K.

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Cultrex 3-D Culture Matrix RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Cultrex 3-D Culture Matrix RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

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