BamHI from Promega

Product Specs
ItemBamHI
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberR6025
Quantity12,500u
Concentration10u/µl

Sequence

G▼GATC C
C CTAG▲G

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) LukS-PV targeting C5aR inhibits EMT in hepatocellular carcinoma via the BCL6/HDAC6/HSPD1 axisCommunications BiologyJanuary 30, 2026Pengsheng Ding, Lan Shi, Xuexue Xu, Bing Lu, Gan Liu, Yangyan Wang, Zhengchao Nie, Xiaofang Wang, Wenjiao Chang, Yuanyuan Dai, et al.protocol. To generate the recombinant LukS-PV-GFP, the LukS-PV sequence was digested with BamHI (Promega, #R6021) and XhoI (Promega, #R6161), then ligated into the pMD-18T vector (Takara, Tokyo, Japan) to produce

(2) An AI-driven workflow for the accelerated optimization of cell-free protein synthesisiScienceOctober 17, 2025Mostafa M. Khalil, Aisha Elsawah, An N. Hoang, Jean-Loup Faulon, Baptiste Panthu, Joan HérissonHis-HiBiT-GFP, His-HiBiT-ColM and His-HiBiT-ColE1 fragment previously digested by BamHI (Promega, #R6021) and EcoRV (Promega, #R6351) restriction enzymes from bacterial pET21 counterparts. Fragments were inserted

(3) Neurovascular pericytes are susceptible to infection by JC polyomavirusJournal of VirologySeptember 23, 2025Bethany A. O'Hara, Kaitlin Garabian, Wenqing Yuan, Walter J. Atwood, Sheila A. Haley(dsDNA) High Sensitivity Quantification Assay (Invitrogen, #Q32851) and digested with BamHI (Promega, #R6021) prior to transfection. Cells were transfected with 2 µg per well using polyethylenimine (PEI, #R6021)

(4) Adeno-Associated Virus-Engineered Umbilical Cord-Derived Mesenchymal Stromal Cells Overexpressing Human sFlt-1 for Anti-AngiogenesisLife (Basel, Switzerland)April 30, 2025Choy EY, Leong CO, Cheong SK, Then KL, Then KY.two restriction enzymes: Nru I (Cat #R7091; Promega Corporation, Madison, WI, USA) and BamH I (Cat #R6021; Promega Corporation, Madison, WI, USA) based on the manufacturer’s protocol. The reaction was incubated

(5) Discovery of a New Class of Thiazolidin-4-one-Based Inhibitors of Human Dihydroorotate Dehydrogenase: Biological Activity Evaluation, Molecular Docking, and Molecular DynamicsACS OmegaApril 1, 2025Xiaoyong Liu, Xiaoli Ren, Xiaoping Ren, Ji Zhang, Miao Hua, Chaoya Sui, Zhonghong Liu, Fen Luo, Sha Ran, Xiangbi Li, et al.purchased from Novagen company. T4 DNA ligase (M1801), Taq enzyme (M7122), restriction endonuclease BamHI (R6021), restriction endonuclease NdeI (R6801), protein marker (V5235S), and DNA marker (G1741) were purchased

(6) Toxin protein LukS-PV targeting complement receptor C5aR1 inhibits cell proliferation in hepatocellular carcinoma via the HDAC7–Wnt/β-catenin axisJournal of Biological ChemistryFebruary 1, 2025Lan Shi, Shanshan Zhang, Gan Liu, Zhengchao Nie, Pengsheng Ding, Wenjiao Chang, Yuanyuan Dai, Xiaoling Maby PCR. PCR products were digested with XhoI (catalog no.: R6161; Promega) and BamHI (catalog no.: R6021; Promega) and ligated into the pET28a vector. Recombinant LukS-PV was generated as described previously

(7) Elastin‐like polypeptide delivery of anti‐inflammatory peptides to the brain following ischemic strokeThe FASEB JournalAugust 1, 2023John Aaron Howell, Nicholas Gaouette, Mariper Lopez, Stephen P. Burke, Eddie Perkins, Gene L. Bidwellencoding the cell penetrating peptide SynB1 underwent restriction digest using BamHI (Promega, catalog #R6021) for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C. DNA cassettes

(8) Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cellsiScienceJuly 21, 2023Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. KilpatrickD3396-01 Wizard SV Gel and PCR Clean-Up System,Promega (USA),Cat# A9281 BamHI enzyme,Promega (USA),Cat# R6021 XhoI enzyme,Promega (USA),Cat# R6161 DH5 alpha Chemically Competent cells,invitrogen,Cat# 18265-017 T4 DNA ligase

(9) Disease parameters following ocular herpes simplex virus type 1 infection are similar in male and female BALB/C micePLOS ONEJune 15, 2023Aaron W. Kolb, Sarah A. Ferguson, Inna V. Larsen, Curtis R. Brandtethanol, followed by resuspension in sterile water. To determine if a strain was a recombinant, BamHI (R6021; Promega, Madison, WI, USA) digested DNA was electrophoresed on a 1% agarose-tris/borate/EDTA (TBE)

(10) Elastin-like polypeptide delivery of anti-inflammatory peptides to the brain following ischemic strokebioRxiv : the preprint server for biologyMarch 15, 2023Howell JA, Gaouette N, Lopez M, Burke SP, Perkins E, Bidwell GL 3rd.encoding the cell penetrating peptide SynB1 underwent restriction digest using BamHI (Promega, catalog #R6021) for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C. DNA cassettes

(11) The influence of 4-thiouridine labeling on pre-mRNA splicing outcomesPLOS ONEDecember 13, 2021Jessie A. C. Altieri, Klemens J. Hertelof the constructs were determined using MaxEntScanner [11]. After linearizing with BamHI (Promega, R6021) and cleaning/concentrating (Zymo, D4013) AdML and β-Globin, a 10μL radioactive in vitro transcription

(12) A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patientsSTAR protocolsSeptember 17, 2021Goh YS, Ng LFP, Renia L.glycerol content.Alternatives: XbaI and BamHI enzymes from other suppliers, such as Promega, (#R6181 and #R6021 respectively) can be used.b.Run the digest on 0.8% agarose TAE gel at 100 V for 90 min.Note: Run 1 kb

(13) Genetic Diversity of Stellate Sturgeon in the Lower Danube River: The Impact of Habitat Contraction upon a Critically Endangered PopulationWaterApril 18, 2021Daniela Nicoleta Holostenco, Mitică Ciorpac, Elena Taflan, Katarina Tošić, Marian Paraschiv, Marian Iani, Ștefan Honț, Radu Suciu, Geta RîșnoveanuAluI (Promega R6281), Tru9I (Promega R7011), BsrI (Promega R7241), BslI (NEB R0555), BamHI (Promega R6021). The restriction fragments were obtained individually for each candidate enzyme. The RFLP reaction

(14) Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated NanobodiesCell chemical biologyOctober 15, 2020Soave M, Heukers R, Kellam B, Woolard J, Smit MJ, Briddon SJ, Hill SJ.R6341 dNTP mix,"Promega, Wisconsin, USA",Cat# U1511 KpnI Restriction Enzyme,"Promega, Wisconsin, USA",Cat# R6021 Pfu DNA Polymerase,"Promega, Wisconsin, USA",Cat# M7741 T4 DNA Ligase,New England Biolabs (UK),Cat#

(15) A two-lane mechanism for selective biological ammonium transporteLifeJuly 14, 2020Gordon Williamson et al.recombinant protein",XhoI,Promega,Cat# - R6161, "Peptide, recombinant protein",BamHI,Promega,Cat# - R6021, Commercial assay or kit,Quikchange XL site-directed mutagensis kit,Agilent Technologies,Cat# 200516, "Chemical

(16) MicroRNA-1275 inhibits cell migration and invasion in gastric cancer by regulating vimentin and E-cadherin via JAZF1BMC CancerDecember 1, 2019Jia-Wei Mei, Zi-Yi Yang, Hong-Gang Xiang, Runfa Bao, Yuan-Yuan Ye, Tai Ren, Xue-Feng Wang, Yi-Jun Shu

(17) Complex Formation between VEGFR2 and the β₂-AdrenoceptorCell chemical biologyJune 20, 2019Kilpatrick LE, Alcobia DC, White CW, Peach CJ, Glenn JR, Zimmerman K, Kondrashov A, Pfleger KDG, Ohana RF, Robers MB, et al.,New England Biolabs (UK),Cat# R0539S BamH1 restriction enzyme,"Promega Corporation (Wisconsin, USA)",Cat# R6021 BglII restriction enzyme,"Promega Corporation (Wisconsin, USA)",Cat# R6081 XhoI restriction enzyme,"Promega Corporation

(18) INT6 interacts with MIF4GD/SLIP1 and is necessary for efficient histone mRNA translationRNA (New York, N.Y.)June 1, 2012Neusiedler J, Mocquet V, Limousin T, Ohlmann T, Morris C, Jalinot P.

(19) RDH10 is the primary enzyme responsible for the first step of embryonic Vitamin A metabolism and retinoic acid synthesisDevelopmental BiologySeptember 15, 2011Krysten M. Farjo, Gennadiy Moiseyev, Olga Nikolaeva, Lisa L. Sandell, Paul A. Trainor, Jian-xing Ma

(20) Mycoplasma Bovis Outbreak in a Herd of North American Bison (Bison Bison)Journal of Veterinary Diagnostic InvestigationSeptember 3, 2010Kyathanahalli S. Janardhan, Mike Hays, Neil Dyer, Richard D. Oberst, Brad M. DeBeyR6011, Promega Corp., Madison, WI. k. Catalog no. R6381, Promega Corp., Madison, WI. l. Catalog no. R6021, Promega Corp., Madison, WI. m. Catalog no. K2500-20, Invitrogen Corp., Carlsbad, CA. References 1.

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BamHI

Description

Sequence

Promega

Citations (28)

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BamHI from Promega

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