BamHI-HF from New England Biolabs

Product Specs
ItemBamHI-HF
CompanyNew England Biolabs
Price $64.00 Inquire
Catalog NumberR3136S
Concentration20,000 units/ml
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G⇓GATC C
C CTAG⇑G

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Assembly of exon junction complexes on spliceosomes begins before spliceosome activationNucleic Acids ResAugust 24, 2026Elizabeth T Abshire, Lynne E Maquatoverhang at the 3′ end. The targeting vector, which derived from pcDNA3 [23], was digested with BamHI-HF (R3136S, New England Biolabs) and HindIII-HF (R3104S, New England Biolabs) according to manufacturer instructions,

(2) Targeting host lipogenesis with a diarylamide inhibitor disrupts SARS-CoV-2 replicationiScienceAugust 21, 2026Xintian Zhang, Tingfu Du, Yongjian Wang, Wenhai Yu, Tanxiu Chen, Ruixue Liu, Yunpeng Liu, Ruimin Zhu, Doudou Xu, Li Li, et al.and the PCR-amplified UTR fragment were double-digested with HindIII (NEB, R3104S) and BamHI (NEB, R3136S) at 37°C for 2 h. The digested products were then ligated using T4 DNA Ligase (NEB, M0202M) at 16°C

(3) The dual specificity phosphatase 2 act as distal regulatory node in T cell signalingiScienceAugust 21, 2026Henrike Bruckmueller, Anangi Balasiddaiah, Sofia Malek, Victoria Tenhaken, Julien Bruckmueller, Jakob Mejlvang, Helene Spangenberg, Farah Syed, Bjarne Johansen, Hanne Kildalsen, et al.Aldrich,K1876 Puromycin,Sigma Aldrich,P8833 SCH772984,"MedChemExpress,",HY-50846 BamH1-HF,New England Biolabs,R3136S EcoRI,New England Biolabs,R0101S Kpn1-HF,New England Biolabs,R3142S XhoI,New England Biolabs,R0146S XbaI,New England Biolabs

(4) Reassessment of the roles of coronin proteins as actin effectors and in signalingPLoS BiolAugust 18, 2026Roko Gvozdenica Šipić, Haiyan Zhang, Andrii Kharin, Jerneja Koren, Viviana D'Otolo, Yuko Nariai, Takeshi Urano, Pawel Pelczar, Klemens Fröhlich, Alexander Schmidt, et al.mEmerald-Coronin1B-N-10 were digested using NheI-HF and BamHI-HF (New England Biolabs, CAT: R3131S; CAT: R3136S). Ligation was performed using T4 DNA ligase (Promega, CAT: M180B). All the DNA separation and isolation

(5) Nanopore-Based Profiling of PEGylation in Nucleic Acid TherapeuticsJ Am Chem SocAugust 12, 2026Gerardo Patiño Guillén, Thieme T. Schmidt, Jeremy J. Baumberg, Jack W. Szostak, Ulrich F. Keyser, Filip Boškovićwith a linear cooling ramp to 25 °C for 40 min. Then, we added to the mixture 1 μL of BamHI-HF (NEB, R3136S) and 1 μL of EcoRI-HF (NEB, R3101S). The reaction mixture was incubated at 37 °C for 1 h and purified

(6) Decoding strain level interactions between Vibrio cholerae and gut microbiotaVirulenceJuly 30, 2026Guozhong Chen, Shucheng Li, Qinghua Zhao, Yanfei Han, Zhiyuan Zhang, Zhi Liu(New England Biolabs (Beijing) Ltd., Cat#R0111S) and BamHI (New England Biolabs (Beijing) Ltd., Cat# R3136S). The katE-like gene sequence was amplified by PCR using FastPfu DNA Polymerase (TransGen Biotech Co.,

(7) ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy developmentCell Reports MedicineJuly 21, 2026Merijn Braams, Martijn Cordes, Sandra A. Vloemans, Bas de Mooij, Sandra de Bruin-Versteeg, Ashley Wachtmeester, Anton W. Langerak, Karin Pike-Overzet, Frank J.T. Staal, Kirsten Canté-Barrett, et al."Fluo-4, AM, cell permeant",Invitrogen,Cat#F14201 Q5® High-Fidelity DNA Polymerase,New England Biolabs,Cat#M0491S BamHI-HF,New England Biolabs,Cat#R3136S NotI-HF,New England Biolabs,Cat#R3189S Lentiboost -P Pharma grade,Sirion,Cat#SB-A-LF-901-01 AmpliTaq

(8) Salmonella Typhi asparaginase-dependent activation of GCN2 promotes bacterial killing in murine macrophagesInfection and ImmunityJuly 14, 2026Zachary M. Powers, Michael J. McFadden, Gi Young Lee, Tracey Schultz, Luiza A. Castro Jorge, Daniel F. Edwards, Simon Sanchez-Paiva, Jonathan Z. Sexton, Katherine R. Spindler, Jeongmin Song, et al.Cap,HS10060,Millipore Sigma Minimum Essential Medium (MEM),11095080,Invitrogen BamHI-HF restriction enzyme,R3136S,New England Biolabs NotI-HF restriction enzyme,R3189S,New England Biolabs Herculase II Fusion DNA

(9) A peptide-based screen for cell death inhibitors identifies the cytoprotective compound CDL36bioRxivJuly 10, 2026Zintis Inde, Sebastian Keppler, Jesse Gelles, Cameron S. Fraser, Adam Presser, Jarvier N. Mohammed, May Jung, David S. Garvey, Tudor Moldoveanu, Jerry E. Chipuk, et al.pENTR_Cit-tevs-DHFR backbone and BimEL PCR product were digested with BamHI and XhoI (New England Biolabs, cat #R3136S, R0146S), gel purified, and ligated in a Quick Ligation reaction. The resulting plasmid (pENTR_BimEL-tevs-DHFR),

(10) Activation of the G-protein coupled estrogen receptor 1 (GPER1) reduces transient receptor potential vanilloid 1 (TRPV1) activity and human iPSC-derived nociceptive neuron firingStem Cell Res TherJuly 7, 2026Oliver Dräger, Angelique Grell, Asaria E. Vogel, Erhard Wischmeyer, Beatrice A. Nossekusing Monarch® PCR & DNA Cleanup Kit (#T1030S, NEB, Ipswich, MA, USA) and digested with BamHI-HF (#R3136S, NEB, Ipswich, MA, USA) and EcoRI-HF (#R3101S, NEB, Ipswich, MA, USA) restriction enzymes. Fragments

(11) Loss of heterozygosity exposes germline mutations in complex I and drives Warburg metabolism in oncocytic carcinoma of the thyroidSci AdvJuly 3, 2026Celia de la Calle Arregui, Anderson R. Frank, Kelvin Mun, Jiwoong Kim, Kuntal Majmudar, Justin A. Bishop, Cheryl Lewis, Yang Xie, David G. McFaddeninto a PGK-IRES-Blast lentiviral vector previously digested with BamHI and XBaI (New England Biolabs, #R3136S and #R0145S) using Gibson Assembly (New England Biolabs, #E2621S), generating the final expression constructs.

(12) Bacterial lipid synthesizing enzymes PlsY and PlsC utilize both stereo-forms of glycerol-phosphateEMBO ReportsJune 12, 2026Philipp Rieche, Mirthe Hoekzema, Sergiy Gan, Greetje A Berrelkamp-Lahpor, Adanna W Ezissi, Marten Exterkate, Arnold J M DriessenO1008, P9417" "Restriction enzymes: NcoI, SacI, BamHI, EcoRI",New England Biolabs,"Cat: R3193S, R3156S, R3136S, R3101S" KLD Enzyme Mix,New England Biolabs,Cat: M0554S Gibson Assembly Mix,New England Biolabs,Cat:

(13) Nociceptor-enriched CAPS1 isoform couples to Ca V 2.2 channels and underlies inflammatory pain hypersensitivitybioRxivMay 30, 2026Melissa Daeschner, Emilio Román Mustafa, Andi Morgan, Quiana Tyss Mosley, Eduardo Javier López Soto127808; a gift from Salvatore Chiantia) were digested with EcoRI-HF (NEB R3101S) and BamHI-HF (NEB R3136S). Digested products were gel-purified using a Qiagen gel extraction kit (Qiagen 28704) and ligated using

(14) HK2 promotes angiogenesis through H3K18 lactylation in peripheral arterial diseaseCommunications BiologyMay 28, 2026Jing Chen, Yue Wang, Xuan Wang, Jin Li, Yu Liu, Jipeng Zhou, Chuanchang Li, Wanzhou Wu, Yongping BaiThe PCR product and plvx-Tet3G vector (Clontech) were digested with EcoRI (#R3101S; NEB) and BamHI (#R3136S; NEB) at 37 °C for 0.5 h, purified using a Gel Extraction Kit (#R3136S; Sangon Biotech), and recombined

(15) Recombinant LGR4 extracellular domain attenuates osteoclastogenesis and prevents bone metastasis via RANKL inhibitionJournal of Bone OncologyMay 19, 2026Meng Fu, Guowei Chang, Shuilin Shao, Liying Zhao(Tiangen, DP209), digested with Hind III (New England Biolabs, R3104S) and BamH I (New England Biolabs, R3136S) for 2 h at 37°C, and ligated into the similarly digested pcDNA4/TO vector (Thermo Fisher, V102020)

(16) Seipin-defined ER sites undergo distinct enrichment of factors for the biogenesis of sterol ester rich lipid dropletsiScienceMay 15, 2026Amit Khatri, Surabhi Yadav, Umair Hashmi, Atul Kumar Dandriyal, Vineet Choudhary,New England Biolabs,Cat# R3104S SpeI-HF,New England Biolabs,Cat# R3133S BamHI-HF,New England Biolabs,Cat# R3136S XhoI,New England Biolabs,Cat# R0146S TRIzol,Thermo Fisher Scientific,Cat# 15596018 Phusion™ High-Fidelity

(17) Formation and function of a novel Atg21-retromer complex in S. cerevisiaeAutophagyMay 15, 2026Noreen Strubel, Jan Förster, Florian Kramer, Michael ThummpGPD1-GFP-Atg21D28R, and pGPD1-GFP-Atg21T106E. The p425GPD backbone was digested with BamHI-HF (NEB, R3136S) and EagI-HF (NEB, R350S), while the inserts were prepared by digesting the respective pUG36 plasmids

(18) The histone gene family in Drosophila simulans carries consistent species-specific nucleotide polymorphisms distinct from that of Drosophila melanogasterMicroPubl BiolMay 15, 2026Sierra Falcone, Annalise Weber, Salaam Awad, Leila Riederprimer/probe master mix and 0.02u/L per sample of restriction enzyme BamH1-HF (New England Biolabs R3136S) into the ddPCR master mix (BioRad 1863023). We selected Bam-H1 because it is predicted to cut once

(19) MICU proteins facilitate calcium-dependent mitochondrial metabolon formation to regulate cellular energetics independently of MCUNature MetabolismMay 13, 2026Henry M. Cohen, Benjamin Gottschalk, Carmen Choya-Foces, Adam Chatoff, Anya Wilkinson, Elena Berezhnaya, Joanne F. Garbincius, Adyson Johnson, Tyler L. Stevens, Jordan E. Howe, et al.by cutting MICU2 and MICU2EFmut out of their respective plasmids using BamHI (New England Biolabs, R3136S) and NsiI (New England Biolabs, R0127S) and ligating the mutant fragment into the WT vector. MICU1/MICU2/MICU3EFmut

(20) STREMI: a dual-function upstream ORF-encoded regulator of mitochondrial cristae architectureEMBO ReportsMay 2, 2026Ruiyang Guo, Yabo Guo, Runguo Shu, Jiajia Qian, Jiawei Wang, Ruobing Li, Ti Qin, Ziyi Wang, Hongtao Tian, Mengchen Wu, et al.restriction enzyme,NEB,Cat#R0146S Quick CIP phosphatase,NEB,Cat#M0525S BamHI-HF restriction enzyme,NEB,Cat#R3136S EcoRI-HF restriction enzyme,NEB,Cat#R3101S SalI-HF restriction enzyme,NEB,Cat#R3138S NheI-HF restriction

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(1)

4/16/2018Diagnostic Restriction Mapping Of Plasmid Constructs Triveni MahendrappaI have constructed a plasmid using pAYC184 as backbone with 16 kb operon making it to 20 kb size. I wanted to quickly check the constructed plasmid with restriction with BamHI and SalI double digest. ... Read Review

BamHI-HF

BamHI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (290)

Reviews

BamHI-HF from New England Biolabs

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