BamHI from New England Biolabs

Product Specs
ItemBamHI
CompanyNew England Biolabs
Price $66.00 Inquire
Catalog NumberR0136S
Concentration20-100 u/µL
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G/GATCC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multiomics Analysis across the Life Cycle Identifies Zn 2 Cys 6 _61 as a Target for Enhancing Triterpenoid Production in Ganoderma lucidumJournal of Agricultural and Food ChemistryApril 1, 2026Yihong Li, Liwei Liu, Miaoqing Li, Jing Xu, Jihong Yang, Xinyu He, Wan Yang, Wei Li, Rui Zhang, Lisa Mao, et al.and cloning it into the pGADT7 vector digested with Sfi I (R0123S, New England Biolabs) and BamH I (R0136S, New England Biolabs). The recombinant prey plasmid was transformed into the bait yeast strain along

(2) Optimized ND4 allotopic expression for gene therapy of Leber’s hereditary optic neuropathyFrontiers in Bioengineering and BiotechnologyMarch 20, 2026Evgeniy V. Lapshin, Alexander D. Egorov, Alexander S. Malogolovkin, Nizami B. Gasanov, Svetlana A. Smirnikhina, Vyacheslav Yu Tabakov, Alexander V. Karabelskyrecommended by the manufacturer of pAAV-CMV-MCS using BamHI and HindIII restriction endonucleases (NEB, cat. R0136S and R0104S). The ligation reaction of the prepared inserts and vectors was performed according to the

(3) Poly(A) variants supporting robust transmission stability in bacteria and high protein expression in animals for mRNA in vitro transcriptionMolecular Therapy Nucleic AcidsMarch 12, 2026Hua Chen, Wei Qin, Hui Bao, Xuwei Chen, Xu Ye, Yi Wang, Liang Liu, Yanguang Zhang, Ying Sun, Tingting Zhang, et al.clones were screened and confirmed by Sanger sequencing at Genewiz. For ORF cloning, BamHI (NEB, Cat# R0136S) and AscI (NEB, Cat# R0558S) were used to digest the vector and insert fragments, respectively. For

(4) Impact of Tau overexpression on DNA replication dynamics in centromeres of human neural progenitor cellsiScienceFebruary 20, 2026Elisa Balzano, Shyam Twayana, Settapong T. Kosiyatrakul, Glennis A. Logsdon, Bhushan L. Thakur, Evan E. Eichler, Bettina Bohl, Philipp Koch, Simone Sidoli, Anupama Kumari, et al.probe-orange labeled Chromosome 21,MetaSystems,Cat#D-0321-100-OR KpnI-HF,NEB,Cat#R3142 BamHI,NEB,Cat#R0136S EcoRV,NEB,Cat#R0195S ,, Deposited data,Deposited data,Deposited data ,, "PacBio HiFi and ONT data

(5) Molecular basis for anti-jumbo phage immunity by AVAST type 5Molecular CellFebruary 19, 2026Aswin Muralidharan, Ana Rita Costa, Desi Fierlier, Daan Frits van den Berg, Halewijn van den Bossche, Adja Damba Zoumaro-Djayoon, Alicia Rodríguez-Molina, Martin Pabst, Martin Pacesa, Bruno E. Correia, et al.UFC903008 Ampicillin,Carl Roth,Cat# K029.4 Biotin,Sigma-Aldrich,Cat# N8878-25G BamHI,New England Biolabs,Cat# R0136S β-Nicotinamide Adenine Dinucleotide Sodium Salt,Sigma-Aldrich,Cat# N0632 Carbenicillin,Fisher Scientific,Cat#

(6) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al.,ThermoFisher Scientific,Cat#FD0304 KpnI,New England Biolabs,Cat#R3142L NdeI,New England Biolabs,Cat#R0111L BamHI,New England Biolabs,Cat#R0136L NcoI,New England Biolabs,Cat#R0193L FastAP,ThermoFisher Scientific,Cat#EF0651 "RNase Inhibitor, Murine",New England Biolabs

(7) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce DempleR0111S) served as a marker for plasmids that failed in the oligo-swapping process, while the BamHI (NEB, R0136S) digestion product served as a marker for plasmids that successfully underwent the oligo-swapping process. Preparation

(8) Metabolic engineering and late-stage functionalization expand the chemical space of the antimalarial premarineosin ACommun ChemDecember 5, 2025Christina M. McBride, Morgan McCauley, Natalia R. Harris, Sahar Amin, Brian J. Curtis, Linnea Verhey-Henke, Awet A. Teklemichael, Erin N. Oliphant, Patricia Dranchak, Katherine L. Lev, et al.in the plasmid pSET152k-kasOp* between the BamHI and EcoRI restriction sites (New England Biolabs, R0136S and R0101S), as previously described30. Synthetic ribosomal binding sites61 were used as overlapping

(9) Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signalingDevelopmental BiologyDecember 1, 2025Agnese Kocere, Elena Chiavacci, Charlotte Soneson, Seth T. Jacobson, Emma N. Harrison, Kevin Manuel Méndez-Acevedo, Jacalyn S. MacGowan, Harrison H. Wells, Max S. Hiltabidle, Azhwar Raghunath, et al.5′-TTGCTAGCTCAGCGACGTCTCCGGTCT-3′ containing a NheI site (underlined). The PCR product was cloned with BamHI (R0136S, NEB), NheI (R3131S, NEB) restriction digest into Tol2kit vector 396 (pCS2FA:Tol2) (Kwan et al., 2007)

(10) Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometrySTAR ProtocolsDecember 1, 2025Matias Cardenas, Sasha Compton, Ashkon Hunt, Pradeep Chopra, Daniel R. Perez, Geert-Jan Boons, Daniela S. Rajaocommercial assays,Critical commercial assays,Critical commercial assays ,, BamHI,New England Biolabs,R0136S EcoRI,New England Biolabs,R0101S KpnI- HF,New England Biolabs,R3142S XhoI,New England Biolabs,R0146S QIAprep

(11) A high-throughput screening approach to discover potential colorectal cancer chemotherapeutics: repurposing drugs to identify novel disruptors of 14-3-3 proteinsCell Death & DiseaseNovember 10, 2025Siyi He, Daniel Meister, Samra Khan, Azam Mohammadzadeh, Luis Delgadillo Silva, Guy A. Rutter, John F. Trant, Gareth E. LimpmCitrine-C1 (Addgene; plasmid #54587) and pmCitrine-N1 (Addgene; plasmid #54594) using EcoRI and BamHI (NEB; #R0136S). This process attached mCitrine, a yellow fluorescent protein (YFP), to the N- and C-termini of BAD,

(12) Enhancing cap-independent translation of linear mRNANat CommunOctober 16, 2025Sebastian Golojuch, Brendan Largey, Afaf H. El-Sagheer, Tom Brownobtained by digesting pRSET-B mCherry plasmid with EcoRI (#R0101S, New England Biolabs) and BamHI (#R0136S, New England Biolabs) restriction enzymes following the manufacturer’s protocol. Gibson-assembly-compatible

(13) DDHD2 provides a flux of saturated fatty acids for neuronal energy and functionNature MetabolismSeptember 30, 2025Saber H. Saber, Nyakuoy Yak, Xuan Ling Hilary Yong, Yih Tyng Bong, Hannah Leeson, Chuan-Yang Dai, Tobias Binder, Siyuan Lu, Reshinthine Purushothaman, An-Sofie Lenaerts, et al.amplifying a PCR fragment of ERGIC53 of the pMXs-IP spGFP-ERGIC53 using restriction enzymes BamHI (NEB, R0136S) and NheI (NEB, R3131S) on the pMXs-IP spGFP-ERGIC53 plasmid. pMXs-IP spGFP-ERGIC53 was a gift from

(14) Role of listeriolysin O and phospholipases C in L. monocytogenes intercellular protrusion dynamics, resolution, and autophagy avoidancemBioSeptember 10, 2025Madalyn Fields, Abigail Spencer, Sara J. Mason, Christopher C. Phelps, Xiaoli Zhang, Amal Amer, Stephanie Seveaustudy,5′-taagcacggccggctaattaagaagataattaactgctaatcc-3′ Restriction enzymes,Restriction enzymes,Restriction enzymes BamHI,New England Biolabs,R0136S EcoRI-HF,New England Biolabs,R3101S ApaLI,New England Biolabs,R0507S KpnI-HF,New England Biolabs,R3142S EagI-HF,New England Biolabs

(15) Aging-associated DNA methylation of LEF1 modulates inflammation and neurodegenerative pathwaysFrontiers in ImmunologyAugust 21, 2025Mengke Chen, Lujie Zhou, Yidan Gao, Yao Zhong, Cheng Chen, Zhicheng Wang, Xiaoning Wang, Rong Xia(BbsI)-PGKpuro2ABFP (50946, Addgene) was linearized using BbsI (R3059L, NEB, Ipswich, USA) and BamHI (R0136S, NEB), followed by gel purification. Two sets of shLEF1 oligonucleotides were synthesized by Tsingke

(16) Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissueCellAugust 21, 2025Reuben A. Saunders, William E. Allen, Xingjie Pan, Jaspreet Sandhu, Jiaqi Lu, Thomas K. Lau, Karina Smolyar, Zuri A. Sullivan, Catherine Dulac, Jonathan S. Weissman, et al.Ligase,NEB,Cat# M0202S T4 Ligase (High Concentration),NEB,Cat# M0202T BstXI,NEB,Cat# R0113S BamHI,NEB,Cat# R0136S Fugene HD,Promega,Cat# E2311 ViralBoost,Alstem,Cat# VB100 Lenti-X™ Concentrator,Takara,Cat# 631232 BSA,Ambion,Cat#

(17) Quantitative essentiality in a reduced genome: a functional, regulatory and structural fitness mapMolecular Systems BiologyAugust 13, 2025Samuel Miravet-Verde, Raul Burgos, Eva Garcia-Ramallo, Marc Weber, Luis Serranoreagents" Puromycin,Gibco,A1113803 Chloramphenicol,Sigma,C0378 Tetracycline,Sigma,T7660 Gentamicin,Sigma,G1397 Anhydrotetracycline,Takara,631310 BamHI,NEB,R0136S EcoRV,NEB,R0195S Phusion High-Fidelity DNA Polymerase,Thermo Scientific,F530S HEPES,Sigma,H4034 Sucrose,Sigma,84097 Glucose,Sigma,G-8270 PPLO

(18) Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assaySTAR ProtocJuly 15, 2025Thomas M. Groseclose, Zoe K. Taylor, Lexy A. Lujan, Taraka Dale, Hau B. Nguyen,New England Biolabs,Cat#R0146L Phosphoric acid for HPLC,Supelco,Cat#49685-100ML BamHI Enzyme,New England Biolabs,Cat# R0136L NdeI Enzyme,New England Biolabs,Cat# R0111L DNaseI Amp grade,Invitrogen,Cat#18068-015 DeepVent exo(−)

(19) PERRC: Protease Engineering with Reactant Residence Time ControlACS synthetic biologyJune 20, 2025Nelson S, Gaza J, Ajayebi S, Masse R, Pho R, Scutero C, Martinusen S, Long L, Menezes A, Perez A, et al.annotation. Subsequently, the new pRK793 with the ENLYFES cut site was digested with BamHI (NEB, cat# R0136S) and XbaI (NEB, cat# R0145S) to linearize it, then the respective protease sequences were inserted using

(20) High-Throughput Indirect Monitoring of TORC1 Activation Using the pTOMAN-G Plasmid in YeastBIO-PROTOCOLJune 20, 2025Melissa Gómez, Guilherme Rocha, Diego Ruiz, Claudio Martínez, Francisco Salinas, Eduardo I. Kessi-Pérez(USBiological, catalog number: A2260) 24. BamHI restriction enzyme (New England BioLabs, catalog number: R0136S) 25. XhoI restriction enzyme (New England BioLabs, catalog number: R0146S) 26. Zymoprep Yeast Plasmid Miniprep I

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BamHI

BamHI from New England Biolabs

Sequence

New England Biolabs

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BamHI from New England Biolabs

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