Anti-DPH1 antibodies are used in the immunodetection of the protein diphthamide biosynthesis 1. In humans, the canonical protein has a reported length of 438 amino acid residues and a mass of 48.1 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is found to be expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. A member of the DPH1/DPH2 protein family, DPH1 is known to catalyze the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. Synonyms for this target antigen include DPH2L, DPH2L1, OVCA1, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1, DPH-like 1, and DEDSSH. DPH1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of DPH1 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.