Anti-nucleoporin 35 antibodies are used for the immunodetection of the protein encoded by the NUP35 gene. In humans, the canonical protein has a reported length of 326 amino acid residues and a mass of 34.8 kDa. Its subcellular localization is in the nucleus and membrane. Alternative splicing is reported to yield 3 different isoforms for this protein. A member of the Nup35 protein family, it is known to function as a component of the nuclear pore complex (NPC). Other names for this target antigen include mitotic phosphoprotein 44, nuclear pore complex protein Nup53, nucleoporin 35kDa, nucleoporin NUP53, and nucleoporin NUP35. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of nucleoporin 35 antibodies have been mentioned in research publications and have associated citations. These antibodies are most commonly used in Western Blot experiments, among other applications.