Anti-histidine-rich glycoprotein antibodies are used for the immunodetection of the protein encoded by the HRG gene. In humans, the canonical protein has a reported length of 525 amino acid residues and a mass of 59.6 kDa. It is known to be a secreted protein. It is reported to be expressed in macrophages and in malignant cells. It is reported to be a plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Post-translational modifications have been described, including N-glycosylation and protein cleavage. Other names for this target antigen include HRGP, THPH11, histidine-proline-rich glycoprotein, and HPRG. Gene orthologs have been identified in the mouse, rat, bovine, chimpanzee and chicken species. Some histidine-rich glycoprotein antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. Immunofluorescence and Immunohistochemistry are also common applications.