Anti-BRF1 RNA polymerase III transcription initiation factor subunit antibodies are used for the immunodetection of the protein encoded by the BRF1 gene. In humans, the canonical protein has a reported length of 677 amino acid residues and a mass of 73.8 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 9 different isoforms for this protein. A member of the TFIIB protein family, it is reported to be a general activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The gene encoding this protein is implicated in Cerebellofaciodental syndrome. Other names for this target antigen include CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, and BRF. Gene orthologs have been identified in the mouse, rat, bovine, frog, chimpanzee and chicken species.