Anti-glycyl-tRNA synthetase 1 antibodies are used for the immunodetection of the protein encoded by the GARS1 gene. In humans, the canonical protein has a reported length of 739 amino acid residues and a mass of 83.2 kDa. Its subcellular localization is in the cytoplasm and is secreted. It is reported to be widely expressed including in the brain and spinal cord. A member of the Class-II aminoacyl-tRNA synthetase protein family, it is known to catalyze the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). The gene encoding this protein is implicated in Charcot-Marie-Tooth disease. Other names for this target antigen include DSMAV, GARS, GlyRS, HMN5, HMN5A, SMAD1, SMAJI, and CMT2D. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.