Anti-H3-4 antibodies are used in the immunodetection of the protein H3.4 histone, cluster member. In humans, the canonical protein has a reported length of 136 amino acid residues and a mass of 15.5 kDa. Its subcellular localization is in the nucleus and chromosomes. It is reported to be expressed in testicular cells. A member of the Histone H3 protein family, H3-4 is an important component of nucleosome. Post-translational modifications have been described, including ubiquitination, acetylation and phosphorylation. Synonyms for this target antigen include H3/g, H3C16, H3FT, H3t, HIST3H3, histone H3.1t, H3 clustered histone 16, and H3.4. H3-4 gene orthologs have been reported in the mouse, rat, bovine and chimpanzee species.