Anti-SPAG16 antibodies are used in the immunodetection of the protein sperm associated antigen 16. In humans, the canonical protein has a reported length of 631 amino acid residues and a mass of 70.8 kDa. Its subcellular localization is in the cytoplasm. Up to 5 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. SPAG16 is known to be involved with cilium assembly. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include WD repeat domain 29, pf20 protein homolog, sperm-associated WD repeat protein, and sperm-associated antigen 16 protein. SPAG16 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some SPAG16 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.