Anti-notchless homolog 1 antibodies are used for the immunodetection of the protein encoded by the NLE1 gene. In humans, the canonical protein has a reported length of 485 amino acid residues and a mass of 53.3 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the NLE1/RSA4 protein family, it is reported to play a role in regulating Notch activity. Other names for this target antigen include notchless protein homolog 1, Notchless gene homolog, and NLE. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. Some notchless homolog 1 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.