Anti-nucleoporin 54 antibodies are used for the immunodetection of the protein encoded by the NUP54 gene. In humans, the canonical protein has a reported length of 507 amino acid residues and a mass of 55.4 kDa. Its subcellular localization is in the nucleus and membrane. Alternative splicing is reported to yield 3 different isoforms for this protein. A member of the NUP54 protein family, it is a known component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane. Post-translational modifications have been described, including O-glycosylation. Other names for this target antigen include 54 kDa nucleoporin, nucleoporin 54kD, nucleoporin 54kDa, and nucleoporin p54. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of nucleoporin 54 antibodies have been mentioned in research publications and have associated citations. These antibodies are most commonly used in Western Blot experiments, among other applications.