SILAC, or Stable Isotope Labeling with Amino acids in Cell culture, is well-known method for investigating cellular protein expression. The principle involves growing two cell populations, one fed with media containing normal amino acids, and another fed with media containing amino acids labeled with stable, non-radioactive isotopes. Once these labeled amino acids are metabolically incorporated, changes in protein abundances can then be quantified by mass spectrometry. Commercially available SILAC growth media typically comes in a kit or in minimal essential media such as MEM, DMEM, or RPMI deficient in certain amino acids.