Antibodies are used in a broad range of research and diagnostic applications for the enrichment, detection, and quantitation of proteins and their modifications. Unfortunately, many antibodies are poorly characterized both initially and between manufacturing lots due to several challenges. Thus, there is a great need to verify that antibodies recognize their intended targets. To address these issues and to assess the binding specificity of Invitrogen™ antibodies produced by Thermo Fisher Scientific, Thermo Fisher Scientific describes a new approach to antibody target verification. This approach uses optimized sample preparation reagents and methods, high- resolution MS instrumentation, and a novel data analysis pipeline to assess antibody specificity for its intended target using immunoprecipitation combined with mass spectrometry (IP-MS).
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