Blocking Buffer for Fluorescent Western Blotting from Rockland Immunochemicals, Inc.

Product Specs
ItemBlocking Buffer for Fluorescent Western Blotting
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMB-070
Quantity500 mL
ApplicationsWestern Blot
Conjugate/Tag/LabelUnconjugated
PurityBlocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an antimicrobial agent
FormatLiquid (sterile filtered)
Concentration1X
StorageStore Blocking Buffer at 4° C prior to opening. DO NOT FREEZE

Specifications/Features

Application Note: Fluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in Western blotting and other applications. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX, Cy2™, Cy3™, Cy3.5™, Cy5™ and Cy5.5™, DyLight™405, DyLight™ 549, DyLight™ 649, DyLight™ 680, and DyLight™ 800 and Alexa Fluor® 488, Alexa Fluor® 532, Alexa Fluor® 546, Alexa Fluor® 647 and Alexa Fluor® 680 have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for Western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in Western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with this product can be dried and are very stable. Membranes that are stored protected from light can be re-washed and/or rescanned.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) ASFV epitope mapping by high density peptides microarraysVirus ResJanuary 2, 2024Cloé Desmet, Bruna Coelho-Cruz, Dora Mehn, Pascal Colpo, Ana Ruiz-MorenoPhosphate-buffered saline (PBS) with 0.05% Tween20, pH 7.4 (washing buffer), Rockland Blocking Buffer MB-070 (blocking buffer), PBS with 0.05% Tween20 and 10% blocking buffer, pH 7.4 (staining buffer), 1 mM Tris

(2) Compromised Blood-Brain Barrier Junctions Enhance Melanoma Cell Intercalation and ExtravasationCancers (Basel)October 20, 2023Federico Saltarin, Adrian Wegmüller, Leire Bejarano, Ece Su Ildiz, Pascale Zwicky, Andréj Vianin, Florentin Spadin, Klara Soukup, Vladimir Wischnewski, Britta Engelhardt, et al.(semi-dry blotting at 50 mA for 45 min), which was then blocked for 1 h in Rockland Blocking Buffer MB-070 from Rockland Immunochemicals Inc. (LubioScience GmbH, Zürich, Switzerland). Antibody incubation was

(3) A Candidate DNA Vaccine Encoding the Native SARS-CoV-2 Spike Protein Induces Anti-Subdomain 1 AntibodiesVaccines (Basel)September 3, 2023Anders Frische, Vithiagaran Gunalan, Karen Angeliki Krogfelt, Anders Fomsgaard, Ria Lassaunièrefor 15 min. The subarrays were subsequently blocked with a blocking buffer (Rockland Blocking Buffer MB-070, Rockland Immunochemicals, Pottstown, PA, USA) for 30 min. To assess unspecific reactions, the peptide array

(4) Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysisiScienceAugust 18, 2023Arnaud N’Guessan, Senthilkumar Kailasam, Fatima Mostefai, Raphael Poujol, Jean-Christophe Grenier, Nailya Ismailova, Paola Contini, Raffaele De Palma, Carsten Haber, Volker Stadler, et al.TWEEN®20, pH 7.4",Millipore Sigma,Cat#PPB005-20PAK Rockland Blocking Buffer,Rockland Immunochemicals,Cat#MB-070 TRIS,ThermoFisher Scientific,Cat#17926 ,, Deposited data,Deposited data,Deposited data ,, Single

(5) Novel anti-CD30/CD3 bispecific antibodies activate human T cells and mediate potent anti-tumor activityFrontiers in ImmunologyAugust 14, 2023Mary L. Faber, Robyn A. A. Oldham, Archana Thakur, Mary Jo Rademacher, Ewa Kubicka, Theresa A. Dlugi, Steven A. Gifford, William M. McKillop, Nathan J. Schloemer, Lawrence G. Lum, et al.and 100 ug/mL in incubation buffer (PBS, pH 7.4 with 0.05% Tween 20 and 10% Rockland blocking buffer MB-070). Microarray read-outs were obtained with an Innopsys InnoScan 710-IR Microarray scanner. Microarray

(6) A zebrafish model of combined saposin deficiency identifies acid sphingomyelinase as a potential therapeutic targetDisease Models & MechanismsJuly 1, 2023Tejia Zhang, Ivy Alonzo, Chris Stubben, Yijie Geng, Chelsea Herdman, Nancy Chandler, Kim P. Doane, Brock R. Pluimer, Sunia A. Trauger, Randall T. Petersonremove the phosphoprotein prior to reblotting. All antibodies were diluted in blocking buffer (Rockland, MB-070). The dilutions used were 1:500-1:1000 for p-p70S6K, 1:3000 for β-actin and 1:1000 for all additional

(7) Seeding the aggregation of TDP-43 requires post-fibrillization proteolytic cleavageNature neuroscienceJune 1, 2023Kumar ST, Nazarov S, Porta S, Maharjan N, Cendrowska U, Kabani M, Finamore F, Xu Y, Lee VM, Lashuel HA.Proteins were transferred onto 0.45-μm nitrocellulose membranes and blocked with Rockland blocking buffer (MB-070). Membranes were immunoblotted overnight at 4 °C with the rabbit polyclonal antibody TMEM239 at a 1:2,000

(8) Differential recognition of influenza A virus H1N1 neuraminidase by DNA vaccine-induced antibodies in pigs and ferretsFrontiers in ImmunologyMay 29, 2023Jeanette Linnea Tingstedt, Christine Stephen, Christian Risinger, Ola Blixt, Vithiagaran Gunalan, Isik Somuncu Johansen, Anders Fomsgaard, Charlotta Polacek, Ria Lassaunière(PBS with 0.005% Tween20, pH 7.4) followed by a 30 minutes incubation with Rockland Blocking Buffer MB-070 (Rockland, USA). All wash and incubation steps were performed on an orbital shaker at 140 rpm. The peptide

(9) Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenatePLOS ONEMay 3, 2023Montana Renae Barrett, Michael Scott Davisbuffer at 30 V and 0.09 mA for 16 h. Membranes were blocked for 1 h using Rockland Blocking Buffer (MB-070), incubated in the target primary antibody for 1 h, and incubated in a secondary antibody (LI-COR IRDye

(10) Machine learning-driven multifunctional peptide engineering for sustained ocular drug deliveryNature CommunicationsMay 2, 2023Henry T. Hsueh, Renee Ti Chou, Usha Rai, Wathsala Liyanage, Yoo Chun Kim, Matthew B. Appell, Jahnavi Pejavar, Kirby T. Leo, Charlotte Davison, Patricia Kolodziejski, et al.or 0.5 µg/ml) in incubation buffer (pH 6.5 PBS with 0.005% Tween 20 and 10% Rockland blocking buffer MB-070) for 45 min at room temperature to examine background interactions and internal quality control. No

(11) MICU1 regulates mitochondrial cristae structure and function independently of the mitochondrial Ca 2+ uniporter channelScience SignalingApril 25, 2023Dhanendra Tomar, Manfred Thomas, Joanne F. Garbincius, Devin W. Kolmetzky, Oniel Salik, Pooja Jadiya, Suresh K. Joseph, April C. Carpenter, György Hajnóczky, John W. Elrodmembranes (EMD Millipore, Cat#IPFL00010). Membranes were incubated in Blocking Buffer (Rockland, Cat#MB-070) for 1h at room temperature and incubated overnight with specific primary antibodies at 4°C. Membranes

(12) MCU gain- and loss-of-function models define the duality of mitochondrial calcium uptake in heart failurebioRxivApril 22, 2023Joanne F. Garbincius, Timothy S. Luongo, Jonathan P. Lambert, Adam S. Mangold, Emma K. Murray, Alycia N. Hildebrand, Pooja Jadiya, John W. Elrodpolyvinylidene fluoride membranes (Millipore #IPFL00010). Membranes were blocked in blocking buffer (Rockland #MB-070) for 1 hour at room temperature, and then incubated overnight at 4°C in primary antibodies diluted in

(13) Neuronal loss of NCLX-dependent mitochondrial calcium efflux mediates age-associated cognitive declineiScienceMarch 17, 2023Jadiya P, Cohen HM, Kolmetzky DW, Kadam AA, Tomar D, Elrod JW.Reagent,Bio-Rad,Cat # 22660 PVDF Immobilon-FL membrane,EMD Millipore,Cat # IPFL00010 Blocking buffer,Rockland,Cat # MB-070 Dihydroethidium (DHE),Thermo Fisher Scientific,Cat #D11347 KCl,Sigma-Aldrich,Cat #P9333 Cresyl Violet,Sigma-Aldrich,Cat#

(14) Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infectionCell reportsMarch 9, 2023Fossat N, Lundsgaard EA, Costa R, Rivera-Rangel LR, Nielsen L, Mikkelsen LS, Ramirez S, Bukh J, Scheel TKH.membrane (BioRad 162-0260) for 1.5 h at 30 V. Membrane was then incubated in blocking solution (Rockland MB-070) overnight at 4°C under constant shaking. The day after, the membrane was incubated with the primary

(15) Evaluation of tumor antigen-specific antibody responses in patients with metastatic triple negative breast cancer treated with cyclophosphamide and pembrolizumabJournal for ImmunoTherapy of CancerMarch 7, 2023Eric D Routh, Mark G Woodcock, Wolfgang Beckabir, Steven P Vensko, Jonathan S Serody, Benjamin G Vincent(12CA5)-DyLight800 (0.1 µg/mL)) in incubation buffer (PBS, pH 7.4 with 0.05% Tween-20+10% Rockland blocking buffer MB-070) to investigate background interactions with linear peptides. Subsequent incubation of peptide microarrays

(16) Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological TestsVirusesFebruary 28, 2023Carlos Roberto Prudencio, Vivaldo Gomes da Costa, Leticia Barboza Rocha, Hernan Hermes Monteiro da Costa, Diego José Belato Orts, Felipe Rocha da Silva Santos, Paula Rahal, Nikolas Alexander Borsato Lino, Pâmela Jóyce Previdelli da Conceição, Cintia Bittar, et al.peptides. The reaction was performed as follows: the slides were blocked with Rockland blocking buffer MB-070 (30 min before the first assay), followed by incubation with 1 μg/mL of mAb 2F2-ZIKV or 10 μg/mL of

(17) IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual ImmunotherapyInternational Journal of Molecular SciencesFebruary 20, 2023Daniele Danella Figo, Priscilla Rios Cordeiro Macedo, Gabriele Gadermaier, Cesar Remuzgo, Fábio Fernandes Morato Castro, Jorge Kalil, Clovis Eduardo Santos Galvão, Keity Souza Santoswith 0.05% Tween 20 (PBS-T), followed by an initial blocking for 30 min with Rockland Blocking Buffer MB-070 (RBB; Rockland Immunochemicals, Royersford, PA, USA). After short rinsing with PBS-T, the array was

(18) The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in miceReproductionFebruary 1, 2023Katharine Cecchini, Adriano Biasini, Tianxiong Yu, Martin Säflund, Haiwei Mou, Amena Arif, Atiyeh Eghbali, Cansu Colpan, Ildar Gainetdinov, Dirk G. de Rooij, et al.membrane (Millipore, IPVH00010). The membrane was blocked with Blocking Buffer (Rockland Immunochemicals, MB-070) at room temperature for 1.5 h and incubated with rabbit polyclonal anti-A-MYB (Sigma, HPA008791; 1:1,000

(19) Peptide microarray analysis of in-silico predicted B-cell epitopes in SARS-CoV-2 sero-positive healthcare workers in Bulawayo, ZimbabweActa TropicaFebruary 1, 2023Arthur Vengesai, Thajasvarie Naicker, Herald Midzi, Maritha Kasambala, Victor Muleya, Isaac Chipako, Emilia Choto, Praise Moyo, Takafira Mduluza0.05% Tween 20) for 10 min, followed by incubation with a blocking buffer (Rockland blocking buffer MB-070) for 30 min. Initially, the peptide microarrays were incubated with secondary antibodies [Goat anti-human

(20) Characterization of huntingtin interactomes and their dynamic responses in living cells by proximity proteomicsJournal of neurochemistryFebruary 1, 2023Xu H, Bensalel J, Raju S, Capobianco E, Lu ML, Wei J.membranes. The membrane was first blocked with blocking buffer for fluorescent western blotting (Cat. No. MB-070, Rockland Inc.) for 2 hours at room temperature, then incubated with primary antibodies diluted in Tris-buffered

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