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  • Good Reagent: CD127 Antibody

    Good Reagent: CD127 Antibody

    BD Biosciences
    Thursday, July 03, 2014
    We selected CD127 Antibody from BD specifically because CD127 is expressed in common lymphoid progenitor cells and can be found in adult bone marrow. Using this product I was able to differentiate ... read more
  • Great Real-Time Machine from a Trusted Company

    Great Real-Time Machine from a Trusted Company

    Life Technologies
    Thursday, June 12, 2014
    I use real-time PCR machine for various purposes, mainly for checking for the knockdown/over expression of various genes after transfection and to quantify the signals for ChIP experiments. We chose ... read more
  • Anti-CTCF IF

    Anti-CTCF IF

    BD Biosciences
    Tuesday, June 10, 2014
    The purpose of this experiment was to identify an antibody that was suitable for detection of CTCF in human cells by indirect immunofluorescence. read more
  • Great SorLA Antibody

    Great SorLA Antibody

    BD Biosciences
    Friday, May 30, 2014
    I work with intracellular protein trafficking, and I used this antibody to stain for endogenous SorLA in rat hippocampal neurons. It gives a nice and specific staining. I tested a range of antibodies,... read more
  • b-Catenin Detection

    b-Catenin Detection

    BD Biosciences
    Friday, May 30, 2014
    b-Catenin is the effector of Wnt pathway. Intracellular localization changes upon cytosolic stabilization in vivo. read more
  • StepOnePlus is a Step Above the Rest

    StepOnePlus is a Step Above the Rest

    Life Technologies
    Friday, April 11, 2014
    Research in our lab focuses on how temperament or personality relates to physiology and health. The larger goal is to determine how and why individuals differ in their behavioral and physiological ... read more
  • Love This Machine!

    Love This Machine!

    Life Technologies
    Friday, April 04, 2014
    We typically perform 20-40 gene expression experiments each week. This machine is fantastic in that you can create templates, drop in your plate and go. You can analyze your data in a variety of ways... read more
  • The Standard for Comparing All Other PCR Instruments

    The Standard for Comparing All Other PCR Instruments

    Roche Applied Science
    Wednesday, April 02, 2014
    Easy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I use to compare all other PCR instruments to for gene ... read more
  • Cell Cycle Analysis. CDK1 Detection by WB

    Cell Cycle Analysis. CDK1 Detection by WB

    BD Biosciences
    Thursday, March 13, 2014
    We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell cycle progression of SNA1 factor. We used this antibody for the ... read more
  • E-Cadh as Epithelial Marker

    E-Cadh as Epithelial Marker

    BD Biosciences
    Tuesday, January 28, 2014
    The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. Cadherins are a group of proteins on the surface of cells that help neighboring cells stick to one ... read more
  • Cell Adhesion and E-Cadherin Antibody Review

    Cell Adhesion and E-Cadherin Antibody Review

    BD Biosciences
    Thursday, January 16, 2014
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of ... read more
  • BD BrdU Flow Kit Review

    BD BrdU Flow Kit Review

    BD Biosciences
    Thursday, August 08, 2013
    Our lab investigates proliferation and cell cycle in murine hematopoietic cells. To do this we perform flow cytometry on cells stained with antibodies against cell surface markers and labeled with ... read more
  • Purified Mouse Anti-human HLA-A2, clone BB7.2 from BD Biosciences

    Anti-human HLA-A2 Antibody

    BD Biosciences
    Wednesday, May 22, 2013
    BB7.2 is an example of a polymorphic antibody that recognizes epitopes carried by subsets of HLA class I variants. The epitope recognized by BB7.2 lies in the alpha-2 domain of the HLA class I ... read more
  • CD15s (CSLEX)-Anti-Sialyl Lewis x Antibody from BD Biosciences

    Anti-Human CD15s from BD Biosciences

    BD Biosciences
    Monday, April 08, 2013
    The antibody is a really great tool to study sialyl lewis x which is an important selectin ligand both in lymphocyte trafficking and hematogenous metastasis of cancer cells. read more
  • Human Th1/Th2 Cytokine Kit II from BD Biosciences

    Th1/Th2 Cytokine Kit II from BD Biosciences

    BD Biosciences
    Friday, March 15, 2013
    The Human Th1/Th2 Cytokine Kit II allows one to gain information about multiple cytokines related to both the Th1 and Th2 responses. The Cytokine Bead Array (CBA) kit used in the assay described ... read more
  • CFX Connect Real-Time PCR Detection System from Bio-Rad

    CFX Connect from Bio-Rad

    Bio-Rad
    Tuesday, March 05, 2013
    The Bio-Rad CFX Connect Real-Time PCR Detection System is a standard, 96 well quantitative PCR detection system that can detect up to two fluorophores at the same time. Detection thresholds for ... read more
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