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  • Very Good Th1/Th2/Th17 Kit

    Very Good Th1/Th2/Th17 Kit

    Wednesday, January 21, 2015
    This kit is very good for detecting human Th1/Th2/Th17 cytokines from the serum as well as cell culture supernatant. read more
  • PE-CF594 anti mouse RORgamma-t for Th17 cells

    PE-CF594 anti mouse RORgamma-t for Th17 cells

    Tuesday, January 13, 2015
    We transfer wild type CD4 T cells to Rag-/- mice to establish a Colitis model and then look at the phenotype of these transferred CD4 T cells. RORgamma-t is required for the differentiation of Th17 ... read more
  • Review of Mouse IFN gamma ELISPOT kit

    Review of Mouse IFN gamma ELISPOT kit

    Friday, December 12, 2014
    The aim of the research is to monitor IFN gamma production in mouse splenocytes in response to vaccination with Newcastle disease virus recombinants expressing HIV gp160 and Gag proteins. I selected ... read more
  • BUV395 amazing addition in the multicolor panel

    BUV395 amazing addition in the multicolor panel

    Thursday, December 11, 2014
    563793 and 563557 BUV provides a new addition in the multi-color flowcytometry panel. I have tried both Anti-mouse CD19 and CD45R (B220) with this channel and both work extremely well . As ... read more
  • Ab used for staining of mouse naive T cells from spleen

    Ab used for staining of mouse naive T cells from spleen

    Friday, December 05, 2014
    Here I am looking at two parameters at the same time (CD25 PE X-axis and Foxp3e450 Y-Axis). This ab was used to stain mouse naive CD4 T cells from the spleen CD3+CD4+CD25-CD44 low CD62L high. ... read more
  • Solid workhorse machine

    Solid workhorse machine

    Life Technologies
    Tuesday, October 07, 2014
    We wanted to perform qPCR testing to determine if treatment of a G-protein-coupled receptor with an agonist would affect the transcription of different genes. This machine worked well for us over ... read more
  • Good Reagent: CD127 Antibody

    Good Reagent: CD127 Antibody

    Thursday, July 03, 2014
    We selected CD127 Antibody from BD specifically because CD127 is expressed in common lymphoid progenitor cells and can be found in adult bone marrow. Using this product I was able to differentiate ... read more
  • Great Real-Time Machine from a Trusted Company

    Great Real-Time Machine from a Trusted Company

    Life Technologies
    Thursday, June 12, 2014
    I use real-time PCR machine for various purposes, mainly for checking for the knockdown/over expression of various genes after transfection and to quantify the signals for ChIP experiments. We chose ... read more
  • Anti-CTCF IF

    Anti-CTCF IF

    Tuesday, June 10, 2014
    The purpose of this experiment was to identify an antibody that was suitable for detection of CTCF in human cells by indirect immunofluorescence. read more
  • Great SorLA Antibody

    Great SorLA Antibody

    Friday, May 30, 2014
    I work with intracellular protein trafficking, and I used this antibody to stain for endogenous SorLA in rat hippocampal neurons. It gives a nice and specific staining. I tested a range of antibodies,... read more
  • b-Catenin Detection

    b-Catenin Detection

    Friday, May 30, 2014
    b-Catenin is the effector of Wnt pathway. Intracellular localization changes upon cytosolic stabilization in vivo. read more
  • StepOnePlus is a Step Above the Rest

    StepOnePlus is a Step Above the Rest

    Life Technologies
    Friday, April 11, 2014
    Research in our lab focuses on how temperament or personality relates to physiology and health. The larger goal is to determine how and why individuals differ in their behavioral and physiological ... read more
  • Love This Machine!

    Love This Machine!

    Life Technologies
    Friday, April 04, 2014
    We typically perform 20-40 gene expression experiments each week. This machine is fantastic in that you can create templates, drop in your plate and go. You can analyze your data in a variety of ways... read more
  • The Standard for Comparing All Other PCR Instruments

    The Standard for Comparing All Other PCR Instruments

    Roche Life Science
    Wednesday, April 02, 2014
    Easy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I use to compare all other PCR instruments to for gene ... read more
  • Cell Cycle Analysis. CDK1 Detection by WB

    Cell Cycle Analysis. CDK1 Detection by WB

    Thursday, March 13, 2014
    We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell cycle progression of SNA1 factor. We used this antibody for the ... read more
  • E-Cadh as Epithelial Marker

    E-Cadh as Epithelial Marker

    Tuesday, January 28, 2014
    The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. Cadherins are a group of proteins on the surface of cells that help neighboring cells stick to one ... read more
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