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  • Human Granzyme B ELISA

    Human Granzyme B ELISA

    eBioscience
    Friday, May 01, 2015
    My research was to look at Granzyme B production in natural killer cells. Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B ... read more
  • Great agarose for DNA gels

    Great agarose for DNA gels

    Lonza
    Wednesday, April 29, 2015
    We routinely use this agarose to run DNA gels, it melts easily and I have made gels as low as 0.7% as well as very thin gels that hold up to the heat of running gels at up to 200V. The gels also show ... read more
  • Cotinine ELISA for mouse

    Cotinine ELISA for mouse

    Abnova Corporation
    Wednesday, April 15, 2015
    We measure cotinine levels in mouse plasma samples immediatley after exposure to E-cigarettes for 3 days (5Hr/day). Samples between control and E-cigarette exposure were used for comparison. read more
  • Pay attention to the dilution factor for this kit

    Pay attention to the dilution factor for this kit

    Hycult Biotech
    Monday, April 06, 2015
    I am interested in the change in EndoCab IgM after 11 weeks of yogurt consumption in humans. My hypothesis is that yogurt consumption can improve intestinal health, therefore reducing trans-location ... read more
  • Lonza ACK Lysis Buffer in the lysis of red blood cells

    Lonza ACK Lysis Buffer in the lysis of red blood cells

    Lonza
    Tuesday, March 24, 2015
    My work focuses on the effects of immunomodulation in Parkinson's disease. Here, Lonza's ACK Lysis Buffer is used to lyse red blood cells from splenocytes. read more
  • Great ELISA

    Great ELISA

    R&D Systems
    Thursday, December 18, 2014
    Useful myostatin ELISAs have been limited in the past. Our group is examining the endocrine responses of myostatin in healthy humans in response to atrophic stimuli. Plasma from healthy humans was run... read more
  • MIF quantitative ELISA

    MIF quantitative ELISA

    BioLegend
    Wednesday, December 03, 2014
    MIF levels have been shown to be high in patients with acute dengue. Therefore, we set out to validate if this can be used as a biomarker to validate presence of severe dengue infection. We ... read more
  • Purification of PCR products for cloning

    Purification of PCR products for cloning

    Promega
    Monday, December 01, 2014
    I routinely cloning PCR products to various plasmids for expression and conditional knock down experiments. I need high purity PCR product for reliable cloning into my target plasmids. I use Promega... read more
  • Cell screening kit: Easy to use, great data

    Cell screening kit: Easy to use, great data

    BioLegend
    Thursday, November 20, 2014
    I found the Biolegend LEGENDScreen incredibly easy to use and following the manufacturer's instructions produced great data to help me characterize new cell populations. Couldn't be happier with the ... read more
  • Quickly assess cell death- Caspase 3/7 activation

    Quickly assess cell death- Caspase 3/7 activation

    Promega
    Thursday, October 30, 2014
    An easy to way to measure cell death in a plate- based assay. This kit allows the scientist to rapidly measure the levels of caspase-3/7 activation in vitro. This assay complements other commonly ... read more
  • Mouse IFN-gamma ELISA MAX™ Standard

    Mouse IFN-gamma ELISA MAX™ Standard

    BioLegend
    Tuesday, October 28, 2014
    To measure the IFN-gamma production in conA activated T cell culture. To set up a measurement system for T cell activation. read more
  • Use of Extan PMP22 Kit by Centogene

    Use of Extan PMP22 Kit by Centogene

    Centogene AG
    Friday, September 19, 2014
    Extan Kit for PMP22 by Centogene was used to identify point mutations responsible for CMT1A / CMT1E. The instructions in the kit for the setting up of experiment were explicit making the kit very easy... read more
  • Quantifying the Terminal Complement Complex (C5b-9) in Plasma

    Quantifying the Terminal Complement Complex (C5b-9) in Plasma

    Monday, September 08, 2014
    Activation of any of the three complement pathways (classical, alternative, or lectin) results in the formation of the terminal complement complex, also called the membrane attack complex, or MAC. The... read more
  • PPi Release Assay Works Well

    PPi Release Assay Works Well

    Lonza
    Monday, September 08, 2014
    I used the Lonza PPi release assay to measure the activity of a pyrophosphorylase. I also compared the activity to a suspect pyrophosphorylase, however it did not have any activity and worked well as ... read more
  • Great Kit for RNA Extraction

    Great Kit for RNA Extraction

    QIAGEN - Sample & Assay Technologies
    Wednesday, August 06, 2014
    Extraction of total RNA from brain tissue for use in qPCR experiments. Selected this product in order to purify RNA extracted using phenol: chloroform extraction. read more
  • Efficient Silica-Based Genomic DNA Extraction from Blood and Tissues

    Efficient Silica-Based Genomic DNA Extraction from Blood and Tissues

    QIAGEN - Sample & Assay Technologies
    Thursday, July 24, 2014
    I use the kit routinely to extract gDNA from turkey liver or blood samples for SNP genotyping. read more
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