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Loading Control for WB: Tubulin
Monday, January 27, 2014
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth ...
Alpha Amylase Detection in Pancreas
Friday, January 24, 2014
a-Amylase is a protein enzyme EC 188.8.131.52 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Pancreatic a-amylase randomly ...
Insulin Detection in Pancreas
Wednesday, December 25, 2013
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and...
Pancreas Metabolism by IHC-Paraffin
Tuesday, December 24, 2013
The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for ...
GFP Antibody for IF
Monday, December 16, 2013
Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ...
ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich
Monday, March 11, 2013
We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ...
Deglycosylation Kit from Sigma-Aldrich
Tuesday, February 19, 2013
The Native Protein Deglycosylation Kit is used to remove N-linked oligosaccharides from glycoproteins under native conditions as opposed to more traditional methods. Glycosylation protein ...
Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting
Tuesday, February 14, 2012
, we analyzed regenerating skeletal muscle, otherwise desmin expression was very low;
desmin is normally expressed by myogenic cell lines / primary cultures. Our results were quite ...
Rabbit Anti-Pan Cadherin Antibody from Sigma-Aldrich, used in Immunofluorescence
Tuesday, January 17, 2012
I observed good staining in human colon epithelium cells and in smooth muscle cells (longitudinal and circular).
SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich
Wednesday, August 11, 2010
Quantitative real-time PCR (qPCR) is fast replacing conventional reverse transcriptase PCR in many molecular biology labs, increasingly reflected by high quality journals adjusting their requirements ...
Molecular Weight Market Kit for Gel Filtration from Sigma-Aldrich
Wednesday, March 24, 2010
Molecular weight markers for gel filtration are essential components for standardization of the column and for size prediction of an unknown sample. In gel filtration, the samples are run through a ...
Disk Spinning Unit (DSU) From Olympus
Olympus America Inc.
Monday, December 14, 2009
The Olympus Disk Spinning Unit (DSU) is a relatively recent technological advancement in the field of microscopy-based cellular research that employs fluorophores. The principle is based upon a small ...
GenElute Plasmid Prep Kit From Sigma-Aldrich
Tuesday, September 29, 2009
In the modern molecular world almost every biology lab today finds itself working with plasmid DNA in some capacity. One of the simplest tasks required is purifying plasmid DNA from small...
REDExtract-N-Amp™ Tissue PCR Kit From Sigma-Aldrich
Wednesday, July 15, 2009
REDExtract-N-Amp™ Tissue PCR Kit is a good kit in my opinion. I am a graduate student. My research involves characterizing the role of two proteins using an...
SIGMAFAST™ BCIP®/NBT B5655 Tablets From Sigma-Aldrich
Wednesday, May 13, 2009
The technique of Western blotting is a laboratory staple of protein expression analysis. After loading protein samples onto an appropriate gel and separation by electrophoresis, the gel is then ...
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Early Detection Helps Manage A Chronic Graft-Vs.-Host Disease Complication
Friday, March 07, 2014
A simple questionnaire that rates breathing difficulties on a scale of 0 to 3 predicts survival in ...
E-Cigarettes: Gateway To Nicotine Addiction For US Teens, Says UCSF Study
Friday, March 07, 2014
E-cigarettes, promoted as a way to quit regular cigarettes, may actually be a new route to ...
New UT Arlington Study Links BPA And Breast Cancer Tumor Growth
Friday, March 07, 2014
UT Arlington biochemists say their newly published study brings researchers a step closer to ...
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