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  • Not useful for looking at brain homogenate

    Not useful for looking at brain homogenate

    Cell Signaling Technology
    Thursday, March 26, 2015
    The phosphorylation of TH at the different serine residues was evaluated in MPTP-intoxicated mice. Following MPTP, brain regions were isolated, homogenized, purified, and run on Western blots. ... read more
  • Works well for brain homogenate (mouse)

    Works well for brain homogenate (mouse)

    Cell Signaling Technology
    Wednesday, March 25, 2015
    This antibody was used to look at changes in tyrosine hydroxylase phosphorylation at serine 31 in mice following MPTP. 7 days post-MPTP brain regions of interest were isolated, homogenized, and ... read more
  • Good NICD1 Antibody

    Good NICD1 Antibody

    Cell Signaling Technology
    Thursday, March 12, 2015
    For this experiment, I was trying to determine if the gamma-secretase inhibitor DAPT, at a concentration of 10 micromolar, was inhibiting the formation of NICD1 in primary human bronchial epithelial ... read more
  • Autophagy Western Blot Antibodies

    Autophagy Western Blot Antibodies

    Cell Signaling Technology
    Wednesday, March 11, 2015
    To elucidate the effect of mechanical ventilation on oxidative stress and autophagy proteins on diaphragm of patients. read more
  • Anti-PIAS1 (D33A7) XP

    Anti-PIAS1 (D33A7) XP

    Cell Signaling Technology
    Friday, March 06, 2015
    I have tried several antibodies to detect PIAS1 (endogenous or in condition of over-expression) in cells. So far, this antibody is the best one for both western blot and immunofluorescence analysis. read more
  • Good antibody for ChIP assay of MeCP2 binding DNA sequence

    Good antibody for ChIP assay of MeCP2 binding DNA sequence

    Cell Signaling Technology
    Wednesday, February 25, 2015
    My focus of work is studying the critical role of MeCP2 (methyl-CpG binding protein 2), whose mutation causes Rett Syndrome, in the development and function of the nervous system. I discovered a novel... read more
  • Great MeCP2 antibody for immunohistochemistry

    Great MeCP2 antibody for immunohistochemistry

    Cell Signaling Technology
    Monday, February 23, 2015
    My focus of work is studying the critical role of MeCP2 (methyl-CpG binding protein 2), which causes Rett Syndrome if mutated, in the development and function of the nervous system. I discovered a ... read more
  • Phospho vimentin S83

    Phospho vimentin S83

    Cell Signaling Technology
    Friday, February 20, 2015
    Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin that will provide us novel... read more
  • Phospho vimentin S56

    Phospho vimentin S56

    Cell Signaling Technology
    Friday, February 20, 2015
    Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin that will provide us novel... read more
  • MeCP2 antibody for immunostaining of culture cells

    MeCP2 antibody for immunostaining of culture cells

    Cell Signaling Technology
    Thursday, February 19, 2015
    The long-term goal of our lab is to understand the molecular and genetic mechanisms underlying brain development and diseases. My focus is studying the critical role of MeCP2 (methyl-CpG binding ... read more
  • Great immunoblotting antibody

    Great immunoblotting antibody

    Cell Signaling Technology
    Thursday, February 12, 2015
    I wanted to quantify GluR4 protein expression in brain tissue homogenates. I initially tried the rabbit polyclonal anti-GluR4 from the same company. When I showed that it did not work, I was sent a ... read more
  • A good antibody for detecting Ubc9

    A good antibody for detecting Ubc9

    Cell Signaling Technology
    Thursday, February 05, 2015
    Protein SUMOylation is an important post-translational modification required for normal physical functions of a cell. Ubc9 is an evolutionarily conserved SUMO E2 enzyme involving in the SUMO ... read more
  • Great loading control antibody

    Great loading control antibody

    Cell Signaling Technology
    Tuesday, February 03, 2015
    I wanted to investigate whether the target proteins had any diurnal variations in olfactory bulb tissue and investigated whether these proteins varied between biological replicates and over the course... read more
  • Nice LC3B antibody for tougher protein

    Nice LC3B antibody for tougher protein

    Cell Signaling Technology
    Tuesday, January 20, 2015
    This antibody was used in a study that investigated the role of LC3-B in autophagy. Several LC3-B antibodies were tested in this study for various applications; the antibody reviewed here (2775) gave ... read more
  • Good Antibody, Needs a lot to detect Western bands

    Good Antibody, Needs a lot to detect western bands

    Cell Signaling Technology
    Tuesday, January 20, 2015
    This product is one of few phospho antibodies on the market for Pin1. It comes as 50ul but about a 1:100 to 1:500 dilution is necessary to detect phosphorylation reliably. The blot is often dirty, ... read more
  • Works for chemiluminescence but not well for fluorescence - Unattributed Review*

    Works for chemiluminescence but not well for fluorescence - Unattributed Review*

    Cell Signaling Technology
    Monday, January 19, 2015
    I wanted to measure GluR2 protein levels in brain tissue homogenates via immunoblot. The bands visible in the blot were not crisp and clear, but still at the appropriate weight. No side bands were ... read more
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