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  • Review of Mouse IFN gamma ELISPOT kit

    Review of Mouse IFN gamma ELISPOT kit

    Friday, December 12, 2014
    The aim of the research is to monitor IFN gamma production in mouse splenocytes in response to vaccination with Newcastle disease virus recombinants expressing HIV gp160 and Gag proteins. I selected ... read more
  • Good Reagent: CD127 Antibody

    Good Reagent: CD127 Antibody

    Thursday, July 03, 2014
    We selected CD127 Antibody from BD specifically because CD127 is expressed in common lymphoid progenitor cells and can be found in adult bone marrow. Using this product I was able to differentiate ... read more
  • Anti-CTCF IF

    Anti-CTCF IF

    Tuesday, June 10, 2014
    The purpose of this experiment was to identify an antibody that was suitable for detection of CTCF in human cells by indirect immunofluorescence. read more
  • Great SorLA Antibody

    Great SorLA Antibody

    Friday, May 30, 2014
    I work with intracellular protein trafficking, and I used this antibody to stain for endogenous SorLA in rat hippocampal neurons. It gives a nice and specific staining. I tested a range of antibodies,... read more
  • b-Catenin Detection

    b-Catenin Detection

    Friday, May 30, 2014
    b-Catenin is the effector of Wnt pathway. Intracellular localization changes upon cytosolic stabilization in vivo. read more
  • Cell Cycle Analysis. CDK1 Detection by WB

    Cell Cycle Analysis. CDK1 Detection by WB

    Thursday, March 13, 2014
    We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell cycle progression of SNA1 factor. We used this antibody for the ... read more
  • E-Cadh as Epithelial Marker

    E-Cadh as Epithelial Marker

    Tuesday, January 28, 2014
    The CDH1 gene provides instructions for making a protein called epithelial cadherin or E-cadherin. Cadherins are a group of proteins on the surface of cells that help neighboring cells stick to one ... read more
  • Cell Adhesion and E-Cadherin Antibody Review

    Cell Adhesion and E-Cadherin Antibody Review

    Thursday, January 16, 2014
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of ... read more
  • Purified Mouse Anti-human HLA-A2, clone BB7.2 from BD Biosciences

    Anti-human HLA-A2 Antibody

    Wednesday, May 22, 2013
    BB7.2 is an example of a polymorphic antibody that recognizes epitopes carried by subsets of HLA class I variants. The epitope recognized by BB7.2 lies in the alpha-2 domain of the HLA class I ... read more
  • CD15s (CSLEX)-Anti-Sialyl Lewis x Antibody from BD Biosciences

    Anti-Human CD15s from BD Biosciences

    Monday, April 08, 2013
    The antibody is a really great tool to study sialyl lewis x which is an important selectin ligand both in lymphocyte trafficking and hematogenous metastasis of cancer cells. read more
  • Human Th1/Th2 Cytokine Kit II from BD Biosciences

    Th1/Th2 Cytokine Kit II from BD Biosciences

    Friday, March 15, 2013
    The Human Th1/Th2 Cytokine Kit II allows one to gain information about multiple cytokines related to both the Th1 and Th2 responses. The Cytokine Bead Array (CBA) kit used in the assay described ... read more
  • SureBlue TMB 1-Component Microwell Peroxidase Substrate for ELISAs From KPL

    SureBlue TMB 1-Component Microwell Peroxidase Substrate for ELISAs From KPL

    KPL
    Thursday, February 21, 2008
    TMB (3,5,3’5’-tetramethylbenzidine) is a popular chromogenic substrate for the detection of the enzyme HRP (horseradish peroxidase), a commonly used antibody label for ELISAs. An ELISA is a very ... read more
  • DAB Reagent Set from KPL

    DAB Reagent Set from KPL

    KPL
    Thursday, August 23, 2007
    DAB (3,3’-diaminobenzidine) is the most commonly used amine substrate for histochemical detection of cell surface and intracellular antigens on frozen or embedded tissue sections, cytospins and ... read more
  • SuperSignal West Pico Chemiluminescent Substrate From Pierce

    SuperSignal West Pico Chemiluminescent Substrate From Pierce

    Thermo Scientific Pierce Protein Research Products
    Friday, February 02, 2007
    Western blot is a technique that was introduced by Towbin, et al. in 1979; today it is routinely used by many laboratories for protein analysis. The whole procedure involves many steps that must be ... read more
  • ChemiLucent™ ECL Detection From EMD Millipore

    ChemiLucent™ ECL Detection From EMD Millipore

    EMD Millipore
    Tuesday, January 02, 2007
    Western blotting is a basic technique, used routinely for the detection of specific proteins after separation with denaturing polyacrylamide gel electrophoresis (PAGE). Many developments during the ... read more
  • BD Biosciences' BD Cytometric Bead Array Kit

    BD Biosciences' BD Cytometric Bead Array Kit

    Thursday, May 20, 2004
    BD Biosciences Pharmingen Cytometric Bead Array is a multiplexed flow cytometric bead assay that is used to quanitate soluble analytes. The system is comprised of 6 spectrally unique beads that are ... read more
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