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  • Pierce’s Restore Western Blot Stripping

    Pierce’s Restore Western Blot Stripping

    Thermo Scientific Pierce Protein Research Products
    Monday, December 19, 2005
    Standard published protocols routinely make note of the stripping and re-probing of Western Blot membranes, a technique that eluded me for many years. Even when I would use the standard beta-... read more
  • Pierce's Tri-Sil Reagent

    Pierce's Tri-Sil Reagent

    Thermo Scientific Pierce Protein Research Products
    Tuesday, December 06, 2005
    With an increasing interest in glycobiology, especially in the area of carbohydrate analysis, characterization and composition of these glycosyl residues has become important. One useful method for ... read more
  • Pierce's Melon Gel IgG Purification Kit

    Pierce's Melon Gel IgG Purification Kit

    Thermo Scientific Pierce Protein Research Products
    Tuesday, October 25, 2005
    The Melon Gel Purification Kit (Pierce) allows for a simpler approach to monoclonal and polyclonal antibody purification from serum. The kit eliminates the need for repeated washings and elutions ... read more
  • Pierce Biotechnologies' Zeba Desalt Spin Columns

    Pierce Biotechnologies' Zeba Desalt Spin Columns

    Thermo Scientific Pierce Protein Research Products
    Tuesday, July 19, 2005
    If you work with purified or partially purified proteins then at one time or another you will need to perform desalting or buffer exchange, processes that are somewhat similar. Ammonium sulfate, for... read more
  • Pierce's Supersignal West Dura HRP Detection Kit

    Pierce's Supersignal West Dura HRP Detection Kit

    Thermo Scientific Pierce Protein Research Products
    Tuesday, June 21, 2005
    Our lab has tested several kits and protocols for chemiluminescent horseradish peroxidase (HRP)-conjugated antibody detection. In terms of sensitivity, cost and overall ease of use, the Supersignal ... read more
  • Pierce's ImmunoPure Immuno-Precipitation Kit

    Pierce's ImmunoPure Immuno-Precipitation Kit

    Thermo Scientific Pierce Protein Research Products
    Wednesday, June 15, 2005
    The development of rugged, reliable and repeatable protein purification and enrichment is essential when performing immunoprecipitation studies. One classic approach is to couple an antibody to a ... read more
  • Pierce’s SuperSignal ELISA Femto Maximum Sensitivity Substrate

    Pierce’s SuperSignal ELISA Femto Maximum Sensitivity Substrate

    Thermo Scientific Pierce Protein Research Products
    Tuesday, May 17, 2005
    The SuperSignal ELISA Femto Maximum Sensitivity Substrate from Pierce Biotechnology is an enhanced chemiluminescent substrate developed for luminometer-based applications. This substrate is specific ... read more
  • Pierce Biotechnologies’ Gelcode Blue Stain

    Pierce Biotechnologies’ Gelcode Blue Stain

    Thermo Scientific Pierce Protein Research Products
    Wednesday, May 04, 2005
    The Gelcode Blue Stain Reagent by Pierce is an affordable, ready-to-use method that effectively cuts down on the time involved for fixing, staining and de-staining gels. It is a single reagent ... read more
  • Pierce Biotechnology's Mem-PER Eurkayotic Membrane Protein Extraction Reagent Kit

    Pierce Biotechnology's Mem-PER Eurkayotic Membrane Protein Extraction Reagent Kit

    Thermo Scientific Pierce Protein Research Products
    Tuesday, March 01, 2005
    The Mem-PER Eurkayotic Membrane Protein Extraction Reagent Kit from Pierce Biotechnologies provides a highly effective two-step method to obtain pure membrane proteins from eukaryotic cells... read more
  • PIERCE Micro BCA™ Protein Assay Kit

    PIERCE Micro BCA™ Protein Assay Kit

    Thermo Scientific Pierce Protein Research Products
    Thursday, January 20, 2005
    The Micro BCA™ Protein Assay Kit from Pierce Biotechnology is a detergent-compatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. An adaptation of ... read more
  • Pierce Biotechnology’s Slide-A-Lyzer

    Pierce Biotechnology’s Slide-A-Lyzer

    Thermo Scientific Pierce Protein Research Products
    Thursday, December 02, 2004
    Pierce’s Slide-A-Lyzer dialysis products can dialyze a wide range of volumes (100 ul – 30 mL) with your choice of several different molecular weight cut offs (MWCO). The dialysis kit comes with both ... read more
  • Pierce Biotechnology’s MemCode Stain For PVDF Membranes

    Pierce Biotechnology’s MemCode Stain For PVDF Membranes

    Thermo Scientific Pierce Protein Research Products
    Thursday, October 07, 2004
    Our lab performs western blotting on a daily basis and we routinely stain the membrane with ponceau S red solution following transfer to determine transfer efficiency. In a search for alternative ... read more
  • Pierce Biotechnology’s HALT Protease Inhibitor Cocktail

    Pierce Biotechnology’s HALT Protease Inhibitor Cocktail

    Thermo Scientific Pierce Protein Research Products
    Tuesday, June 22, 2004
    Degradation of target proteins by proteases is a constant danger when working with and analyzing proteins. Inhibition of intracellular proteases is necessary to maintain and preserve cellular protein ... read more
  • The EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit from Pierce Biotechnology

    The EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit from Pierce Biotechnology

    Thermo Scientific Pierce Protein Research Products
    Wednesday, September 17, 2003
    Enzyme-linked immunosorbent assays (ELISAs) that are intended to quantitate peptides or proteins in biological fluids must be very sensitive and have minimal cross-reactivity with non-target molecules... read more
  • Pierce's NAb Protein A Spin Chromatography Kit

    Pierce's NAb Protein A Spin Chromatography Kit

    Thermo Scientific Pierce Protein Research Products
    Wednesday, April 30, 2003
    I recently had the occasion to purify the IgG fraction from a small volume of rabbit serum in order to generate a more concentrated solution of antibody for a specific application... read more
  • Pierce’s Y-DER™ Yeast DNA Extraction Reagent

    Pierce’s Y-DER™ Yeast DNA Extraction Reagent

    Thermo Scientific Pierce Protein Research Products
    Wednesday, September 12, 2001
    The Y-DER Reagent is optimized for use on Saccharomyces cerevisiae, but can be used with other organisms. DNA can be obtained from single colonies, fresh or frozen cells or cell pellets from liquid ... read more
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