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  • Cell screening kit: Easy to use, great data

    Cell screening kit: Easy to use, great data

    BioLegend
    Thursday, November 20, 2014
    I found the Biolegend LEGENDScreen incredibly easy to use and following the manufacturer's instructions produced great data to help me characterize new cell populations. Couldn't be happier with the ... read more
  • Excellent Ab for intracelluar staining of mouse IL-10

    Excellent Ab for intracelluar staining of mouse IL-10

    BioLegend
    Friday, November 14, 2014
    We are looking at the intracellular staining of mouse IL-10 in mouse CD4 T cells from the spleen when stimulated by PMA, Inomycin and BFA. It works great. read more
  • CD3 Antibody works at high concentration

    CD3 Antibody works at high concentration

    BioLegend
    Thursday, November 06, 2014
    We are trying to sort CD3 population in mouse spleen cells by Brilliant Violet 650™ anti-mouse CD3 Antibody. read more
  • Excellent Ab for intracellular staining of mouse IFN-g

    Excellent Ab for intracellular staining of mouse IFN-g

    BioLegend
    Thursday, November 06, 2014
    We are looking at the intracellular staining of mouse IFN-gamma in CD4 memory T cells when stimulated by PMA, Ionomycin and BFA. read more
  • Very good CD304 antibody

    Very good CD304 antibody

    BioLegend
    Monday, November 03, 2014
    We use this antibody to discriminate between thymus derived versus peripheral induced regulatory T cells in mouse spleen on a regular basis. It works like a charm. It has very good quality and can be... read more
  • Mouse TNF-alpha ELISA MAX™ Standard

    Mouse TNF-alpha ELISA MAX™ Standard

    BioLegend
    Friday, October 31, 2014
    Detect TNF-a production in LPS activated macrophage culture. It is an easy way to set up this traditional measure system. read more
  • Mouse IFN-gamma ELISA MAX™ Standard

    Mouse IFN-gamma ELISA MAX™ Standard

    BioLegend
    Tuesday, October 28, 2014
    To measure the IFN-gamma production in conA activated T cell culture. To set up a measurement system for T cell activation. read more
  • Excellent mouse IL-7 cytokine

    Excellent mouse IL-7 cytokine

    BioLegend
    Thursday, October 23, 2014
    Basically, we are culturing one mouse B cell line which needs IL-7 for survival and proliferation. At the beginning, we used 10ng/ml, and then we decreased to 5ng/ml and the cells still grew "crazily"... read more
  • PE/Dazzle™ 594 anti-human CD19 Antibody

    PE/Dazzle™ 594 anti-human CD19 Antibody

    BioLegend
    Friday, September 26, 2014
    Cells Depot™ preserved human PBMCs was stained with PE/Dazzle™ 594 anti-human CD19 Antibody diluted in flow cytometry buffer (PBS + 2% CS). A clear separation between positive and negative population ... read more
  • Use of Tendem Dye for Multicolor Flow Cytometry

    Use of Tendem Dye for Multicolor Flow Cytometry

    BioLegend
    Friday, August 15, 2014
    The aim of my study was to investigate CD4+ T cell proliferation. I harvest mouse splenocytes and use following antibodies from Biolegend to isolate CD4+ T cells and B cells: APC conjugated anti ... read more
  • Intracellular IFN-g Staining for Mouse Cells by Flow Cytometry

    Intracellular IFN-g Staining for Mouse Cells by Flow Cytometry

    BioLegend
    Wednesday, August 06, 2014
    Mouse splenocytes were activated by anti-CD3/anti-CD28 for 96 hours, in polarizing conditions towards Tc1/Th1 or Tc17/Th17, followed by restimulation with PMA/Ionomycin in presence of brefeldin-A (... read more
  • CD9: Just Great

    CD9: Just Great

    BioLegend
    Wednesday, July 23, 2014
    We selected CD9 Antibody from Biolegend specifically because CD9 is expressed in some myeloid progenitor cells and can be found in bone marrow. read more
  • CD16/32: Good Reagent

    CD16/32: Good Reagent

    BioLegend
    Tuesday, July 22, 2014
    We selected CD16/32 Antibody from Biolegend specifically because CD16/32 is expressed in myeloid progenitor cells and can be found in bone marrow. read more
  • CD41: Very Useful Reagent

    CD41: Very Useful Reagent

    BioLegend
    Monday, July 21, 2014
    We selected CD41 Antibody from Biolegend specifically because CD41 is expressed in some progenitor cells and can be found in bone marrow. read more
  • CD48: Dim Fluorochrome

    CD48: Dim Fluorochrome

    BioLegend
    Friday, July 18, 2014
    We selected CD48 Antibody from Biolegend specifically because CD48 is expressed in myeloid cells and can be found in bone marrow. read more
  • CD115: Good and Useful Reagent

    CD115: Good and Useful Reagent

    BioLegend
    Thursday, July 17, 2014
    We selected CD115 Antibody from Biolegend specifically because CD115 is expressed in bone marrow progenitor cells and can be found in adult bone marrow. Using this product I was able to differentiate ... read more
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