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  • Simple & Accurate Low Concentration of RNA Measurement

    Simple & Accurate Low Concentration of RNA Measurement

    Life Technologies
    Wednesday, June 18, 2014
    In order to measure the RNA quality by Bioanalyzer, I needed to know the concentration of RNA to choose right chip for Bioanalyzer. Nanodrop is inaccurate for low concentration measurement of RNA. ... read more
  • Great Equipment to Measure Low Quantities of DNA/RNA

    Great Equipment to Measure Low Quantities of DNA/RNA

    Life Technologies
    Wednesday, June 11, 2014
    Our lab does a lot of ChIP-seq and other high throughput experiments (4C, RNA-seq etc). Precise quantification of the amount of nucleic acids in the samples in question is one of the pre-requisites of... read more
  • Akt 2 Detection

    Akt 2 Detection

    Cell Signaling Technology
    Tuesday, June 03, 2014
    Epithelial-mesenchymal transition is a cellular process in which the repressor SNA1 transcription factor regulates negatively E-Cadh promoter. Findings of our lab demonstrate the mechanism is which ... read more
  • Acetylated-Lysine Antibody

    Acetylated-Lysine Antibody

    Cell Signaling Technology
    Friday, May 09, 2014
    Here, I showed the simple and easy way to detect modification of protein by acetylation. read more
  • Solid Antibody for Detecting Endogenous mTOR

    Solid Antibody for Detecting Endogenous mTOR

    Cell Signaling Technology
    Friday, May 09, 2014
    We use Cell Signaling's Rabbit mTOR antibody repeatedly in our lab's work studying the mTOR signaling pathway and its role as a target in biologically directed therapy. read more
  • DDB-2: Excellent Antibody

    DDB-2: Excellent Antibody

    Cell Signaling Technology
    Thursday, May 08, 2014
    DDB2 is DNA Damage Binding protein. Its expression is downregulated in several cancers. We have been able to detect this protein very easily with this antibody by western blot in many mammalian cell ... read more
  • Specific P62 Antibody

    Specific P62 Antibody

    Cell Signaling Technology
    Monday, April 14, 2014
    Highly specific p62 antibody read more
  • Clathrin Heavy Chain Antibody

    Clathrin Heavy Chain Antibody

    Cell Signaling Technology
    Monday, March 10, 2014
    A clean antibody to detect Clathrin heavy chain. High specificity but sensitivity is okay, load 30-40 ug total protein to get a strong signal. read more
  • LC3B Antibody for Tissue Staining

    LC3B Antibody for Tissue Staining

    Cell Signaling Technology
    Wednesday, February 26, 2014
    I have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagososmes. The cell signaling LC3B D11 antibody works great with almost no background, very specific staining. It ... read more
  • Great Cleaved Caspase 3 Antibody

    Great Cleaved Caspase 3 Antibody

    Cell Signaling Technology
    Friday, February 07, 2014
    Experiments were aimed at detecting apoptotic cells in postnatal mouse brain tissue. Cleaved Caspase 3 is a well-known marker for cells undergoing apoptosis in the caspase-dependent pathway. read more
  • PTEN Detection by WB

    PTEN detection by WB

    Cell Signaling Technology
    Thursday, January 23, 2014
    PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of ... read more
  • Akt Pathway Activation Analysis

    Akt Pathway Activation Analysis

    Cell Signaling Technology
    Wednesday, January 22, 2014
    Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major focus of attention because of its critical regulatory role ... read more
  • Akt Detection in MDCK Cells

    Akt Detection in MDCK Cells

    Cell Signaling Technology
    Tuesday, January 21, 2014
    Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function ... read more
  • Excellent Anti Phospho ERK1/2 Antibody for WB

    Excellent Anti Phospho ERK1/2 Antibody for WB

    Cell Signaling Technology
    Wednesday, January 08, 2014
    The goal was to test ERK1/2 activation in primary mouse cardiac cells after growth factors (FGF, PDGF and EGF) stimulation. As shown on the Western blot, 5 minutes of growth factor stimulation led to ... read more
  • IRF3 Activation Measurement During Viral Infections

    IRF3 Activation Measurement During Viral Infections

    Cell Signaling Technology
    Thursday, October 31, 2013
    The goal of this experiment was to compare antiviral signalling between two sets of MEFS (WT vs KO). MEFs were infected with Sendai virus and antiviral signalling was monitored by measuring the ... read more
  • Calcium activity by measuring Phospho-CaMKII

    Calcium activity by measuring Phospho-CaMKII

    Cell Signaling Technology
    Tuesday, September 24, 2013
    Purpose of experiment was to look at intracellular calcium activity by measuring the activation of p- CaMKII. between two cell populations ( WT and KO MEFS). Lane 1, 4: Control Lane 2, 5: ... read more
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