BD Biosciences
BD Horizon™ BUV737 Hamster Anti-Mouse CD80
612773
16-10A1
Info:Supplier Page
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The BUV737 CD80 antibody works poorly for identifying CD80 expression on myeloid immune cells from cell populations isolated from mouse lungs and bronchoalveolar lavage fluid. I found this antibody to fail to provide clear shifts even at 1:100 dilution.
Flow cytometry
Mouse BALF, Lungs
1:100, 30 mins at 4 degrees C in the dark
Fc Shield
BD cytofix/cytoperm; 100 ul for 20 mins at 4C
None
5 laser Aurora Flow cytometer
I used this product to identify shifts in CD80 expression on dendritic cells, alveolar macrophages and monocytes isolated from the lung of unimmunized versus intranasally immunized mice. Cells were isolated from BALF using 1 ml of 1% BSA in PBS and from lungs through collagenase digestion; RBC lysis was performed using ACK buffer. Cells were then stained with viability dye followed by extra-cellular antibodies such as CD45, CD90.2, CD19, Siglec-F, CD11b, CD11c, Ly6C, Gr1, MHCII, MHCI. I think there may be spillover issues with this antibody and fluorophore combination.
N/A
Not recommended.
Doesn't seem to work.
I would not recommend the BUV737 antibody for identifying shifts in co-stimulatory molecule expression in cells.