Affordable and Cheap SYBR Green Reagent for qPCR

February 21, 2024

NTHU
IMCB
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

Maxima SYBR Green/ROX qPCR Master Mix (2X)

Catalog Number:

K0222

Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only templates and primers need to be added.

Experimental Design and Results Summary

Application

qPCR analysis

Starting Material

DNA template and primers

Tips

Only the template and then primers are required to be added

Results Summary

The reagent is easy to use and hassle free. It provides fast and accurate results for our analysis. The product is offered at a very competitive price compared with other similar products available in the market.

DOI or PMID #

N/A

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Summary

The Good

Cheap and does not require the use of specific probes like Tasman.

The Bad

Can only be used for the detection of a single probe.

The Bottom Line

The reagent is easy to use and results are highly reproducible. It is also offered at a very competitive price. Therefore, this SYBR green is highly recommended to those who run qPCR analysis on a daily basis.

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