Fluorescent probe for measuring membrane order

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

Di-4-ANEPPDHQ

Catalog Number:

D36802

I have used Di-4-ANEPPDHQ for measuring membrane order under various conditions. I use a fluorescent ratiometric approach to calculate membrane order using this probe.

Experimental Design and Results Summary

Application

Measuring membrane order

Starting Material

Cell

Tips

Stain at 4 degrees C to avoid endocytosis during staining

Results Summary

The images show plasma membrane stained with Di-4-ANEPPDHQ and imaged at two different emission wavelength.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Low endocytosis.

The Bad

None.

The Bottom Line

Good probe to measure plasma membrane order in cells and liposomes.

Join the discussion