PCR Cloning Kit From Invitrogen for Sequencing of PCR Products

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

TOPO TA Cloning Kit for Sequencing

Catalog Number:

450030

I effectively employed the TOPO TA Cloning Kits to insert Taq polymerase directly–amplified PCR products into a sequencing-ready plasmid vector.

Experimental Design and Results Summary

Application

Cloning Kit

Starting Material

PCR product

Tips

None

Results Summary

I achieved successful outcomes with this kit using a straightforward strategy for the direct integration of PCR products into a plasmid vector, that helped me to do Sanger sequencing.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Straightforward and easy to use kit that works with any PCR products.

The Bad

None.

The Bottom Line

Enables seamless integration of Taq polymerase–amplified PCR products into a sequencing-ready plasmid, featuring an efficient 5-10 minute protocol.

Join the discussion