Efficient and Specific cDNA Synthesis Can be Achieved With the First-Strand Synthesis System From Invitrogen

Stanford University
Biochemistry
Postdoctoral Researcher

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Company:

Invitrogen

Product Name:

SuperScript III First-Strand Synthesis System

Catalog Number:

18080051

I used the SuperScript III First-Strand Synthesis System for RT-PCR because it provides efficient cDNA synthesis from both poly(A)+ and total RNA. The system is very flexible in terms of the choice of primers and compatibility with various DNA polymerases for subsequent PCR amplification.

Experimental Design and Results Summary

Application

Reverse Transcription

Starting Material

poly(A)+ or total RNA

Tips

None

Results Summary

I utilized this system for specific cDNA synthesis from diverse RNA sources, which enabled sensitive detection of RNA targets across a wide size range.

DOI or PMID #

N/A

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Summary

The Good

Efficient and specific cDNA synthesis with enhanced thermal stability for diverse RNA targets.

The Bad

None.

The Bottom Line

Versatile and reliable choice for my experiments with reverse transcription.

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