Kit From Thermo Fisher Efficiently Labels Protein to Read-Out by Flow Cytometry

February 08, 2024

George Washington University
Immunology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

DyLight™ 650 Microscale Antibody Labeling Kits

Catalog Number:

84536

I had a recombinant protein that I wanted to use to test the binding affinity of my T cell to that protein. This kit allowed me to accomplish this all within a matter of hours and produced a clear and quality signal.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Recombinant protein

Tips

Follow directions as stated; I would not use more protein than suggested in protocol (100 µg)

Results Summary

This kit allowed me to conjugate my protein with DyLight 650, which allowed me to measure binding ability of my T cell by flow cytometry on the APC channel.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Quick and instructions are straightforward.

The Bad

None experienced.

The Bottom Line

Overall, I found this kit to be easy-to-follow and efficient in conjugating my protein to DyLight 650. I would recommend this kit to others and will likely try other fluorophores in this line.

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