Pan-DC Enrichment Kit From Miltenyi Works for Whole Blood, but Does Not Work as Well With Other Tissues

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Pan-DC Enrichment Kit, human

Catalog Number:

130-100-777

Our goal was to isolate dendritic cells from tumor cells and evaluate dendritic cell transcriptomic response to different treatments.

Experimental Design and Results Summary

Application

Isolating dendritic cells using negative isolation

Starting Material

Cells

Tips

Do not use with very large cells/particulates

Results Summary

This product helped us isolate a small fraction of dendritic cells which would later be sequenced for transcriptomic analysis.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use.

The Bad

Low recovery.

The Bottom Line

Good product if using whole blood or small cell sizes, performs poorly when using co-cultures or large particles since the filter gets clogged.

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