DiO Labeling for Exosome With the Vybrant™ DiO Cell-Labeling Solution

Università degli Studi Milano-Bicocca
Industrial Biotechnologies
Graduate Student

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Company:

Invitrogen™

Product Name:

Vybrant™ DiO Cell-Labeling Solution

Catalog Number:

V22886

Development of a temperature-sensitive hydrogel (Pluronic) incorporating exosomes to enhance targeting precision and elevate the therapeutic efficacy of exosomes.

Experimental Design and Results Summary

Application

DiO was used to label cellular membrane of exosomes to visualize their dispersion in the hydrogel

Starting Material

Exosomes

Tips

We used a concentration of 8 ul/ml

Results Summary

DiO staining of exosomes allowed us to visualize their dispersion in the hydrogel under confocal microscopy.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy and versatile.

The Bad

None.

The Bottom Line

The dye efficiently targeted cell membranes, resulting in vivid and sharp fluorescence under the microscope. The bright and intense signals were consistent.

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