Excellent B Cell Purification With the Kit From Miltenyi Biotec

December 24, 2023

Deutsches Rheuma-Forschungszentrum
Charite Strasse 1
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Mouse B cell purification kit

Catalog Number:

130-090-862

We are studying the pathways of B cell activation leading to different antibody-producing cell differentiation. For this, we test different adjuvants in vitro, on purified mouse B cells.

Experimental Design and Results Summary

Application

B cell purification for in vitro assay

Starting Material

Mouse splenocytes

Tips

It is important to organize all reagents and materials to work fast , and in the hood to preserve sterile conditions.

Results Summary

The purification kits yields good quality (unactivated) B cells with high degree of purity (>96%), almost as pure as fow cytometric sort with much faster procedure.

DOI or PMID #

N/A

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Summary

The Good

It is fast and yield good purity population.

The Bad

There is considerable loss of B cells in the flow through.

The Bottom Line

The purity of the cells is very satisfactory for in vitro applications. It is also good to perform enrichment before cytometric sort, in case the cells are needed for deep sequencing. The protocol has been improved along the years and is much faster now. It is good value for money, in particular in institute were the sort facility is expensive.

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