Excellent Cell Digestion Buffer from Promega

OMRF
Cardiovascular Biology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

Cell Lysis buffer

Catalog Number:

E153A

Our cell culture experiments use lysis buffers to disrupt the cell membrane. Lysis buffers break the cell membrane by changing the pH. Detergents can also be added to cell lysis buffers to solubilize the membrane proteins and to rupture the cell membrane to release its contents. This lysis buffer works best and gives reproducible results.

Experimental Design and Results Summary

Application

Protein Isolation

Starting Material

Cell culture samples

Tips

Reconstitute the reagent at the time of use.

Results Summary

This method works well for high protein concentrations. We get good results for protein isolation and reproducible results.

DOI or PMID #

N/A

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Summary

The Good

Works,and produces reproducible results.

The Bad

None.

The Bottom Line

This Cell lysis buffer is highly recommended to others.

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