Novus Biologicals
Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [Alexa Fluor® 488]
NBP2-52709AF488
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We are trying to determine the 50% effective concentration value (EC50) of a certain compound against Dengue virus by immunofluorescence assay. We used this primary antibody from Novus, it is conjugated to Alexa Fluor-488 and it targets the envelope protein of flavivirus. During our assay optimization, we determine optimal viral dilution correspondent to 15% of infected cells. We use 1:1000 dilution and it works well and gives a reliable signal.
Immunofluorescence
A549 cells infected with Dengue virus 2 (New Guinea C strain)
1:1000, 1h, room temperature, constant shaking
PBS, 1% Fetal bovine serum, 0.05% Tween-20
Not necessary
Fluorescence signal.
This assay allowed us to determine 50% effective concentration (EC50) of an antiviral compound against Dengue virus. We added different compound concentrations to virus and correlated fluorescence signal to compound concentration, which generated a dose-response curve and corresponding EC50 value.
N/A
Good and stable signal.
None.
I recommend this product, it worked very well for our purpose.